zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1004Research ArticleСтатьиГлубинная УФ-светодиодная динамическая оптическая визуализация и флуоресцентная спектроскопия короны белка в плазмонном растворе и эффект нагрева излучением лазера в ближней ИК-областиDeep UV Led Dynamic Optical Imaging and Fluorescence Spectroscopy of the Protein Corona in a Plasmonic Solution and the Effect of Near-Infrared Laser HeatingKhosroshahiМ. Е.KhosroshahiM. E.

Онтарио

Ontario

khosrom@mie.utoronto.caWoll-MorisonV.Woll-MorisonV.

Онтарио

Ontario

M.I.S. Electronics Inc.; Университет ТоронтоNanobiophotonics & Biomedical Research Laboratory, M.I.S. Electronics Inc., Richmond Hill; Department of Mechanical and Industrial Engineering, University of TorontoM.I.S. Electronics Inc.Nanobiophotonics & Biomedical Research Laboratory, M.I.S. Electronics Inc., Richmond Hill202223032022892210220Copyright © Khosroshahi М.Е., Woll-Morison V., 20222022Khosroshahi М.Е., Woll-Morison V.Khosroshahi M.E., Woll-Morison V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1004

Исследовано взаимодействие светодиодного глубинного УФ-излучения (275 нм) с бычьим сывороточным альбумином (БСА), содержащим наночастицы золота (НЧЗ), с использованием динамического профилирования луча и флуоресцентной спектроскопии. Тепловое воздействие излучения диодного лазера с l = 800 нм на биоплазмонный раствор исследовано с помощью мониторинга зондирующего луча и ИК-камеры. Результаты показали последовательное нелинейное и колебательное поведение комплекса НЧЗ-БСА из-за процесса адсорбции и десорбции белка. Наблюдаемые усиление и тушение флуоресценции тирозина (Tyr) могут дать информацию о кинетике связывания и конформационных изменениях белка. После лазерного нагрева в течение 10 мин флуоресценция Tyr полностью исчезает и зондирующий пучок He–Ne расширяется на ~4 нм из-за столкновений молекул и денатурации белка. Изменение температуры вследствие разворачивания и денатурации белка демонстрирует аналогичную нелинейную картину при разных объемах НЧЗ. Однако температура ниже при более высоких концентрациях НЧЗ, что указывает на более высокую скорость адсорбции белка, способствующую уменьшению локализованного поверхностного плазмонного резонанса посредством нагревания.

When plasmonic nanoparticles (PNPs) enter a biofluid, the adsorbed biomolecules on the surface form a protein corona, which has direct consequences in biomedical applications. The binding in passive adsorption is nonspecific and is governed by ionic, van der Waals, and hydrophobic forces. We describe the results of deep LED (275-nm) interaction with bovine serum albumin (BSA) containing gold nanourchin (GNU) using dynamic beam view profiling and fluorescence spectroscopy. 800-nm diode laser-induced thermal effects on the bioplasmonic solution were investigated using probe beam monitoring and an IR camera. The results indicated a consistent nonlinear and oscillatory behavior of the GNU-BSA complex due to the adsorption and desorption process of protein. Tyrosine (Tyr) fluorescence enhancement and quenching were observed, which can provide some information about the binding kinetics and protein conformational changes. After 10 min of laser heating, the Tyr fluorescence completely vanished and the He-Ne probe beam was broadened by about 4 nm owing to molecular collisions and protein denaturation. The temperature variation due to protein unfolding and denaturation exhibited a similar nonlinear pattern at different GNU volumes. However, the temperature was lower at higher GNU concentrations, indicating a higher rate of protein adsorption, which effectively mitigated the localized surface plasmon resonance heating.

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The authors declare that there are no conflicts of interest present.