zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1015Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESРазработка и валидация УФ-спектрофотометрического метода оценки синтезированного комплекса лентинан–конго красныйDevelopment and Validation of a UV Spectrophotometric Method for the Estimation of the Synthesized Lentinan–Congo Red ComplexTrivediS.TrivediS.

 Нагпур, Махараштра 

 Nagpur, Maharashtra 

sagartrivedi2000@gmail.comBelgamwarV.BelgamwarV.

 Нагпур, Махараштра 

 Nagpur, Maharashtra 

WadherK.WadherK.

Кампти Нагпур, Махараштра

 Kamptee Nagpur, Maharashtra 

UmekarM.UmekarM.

Кампти Нагпур, Махараштра

 Kamptee Nagpur, Maharashtra 

Университет Раштрасанта Тукадоджи Махараджа НагпураDepartment of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University CampusФармацевтический колледж Кишоритай БхоярDepartment of Pharmaceutics, Smt. Kishoritai Bhoyar College of Pharmacy202224032022892285285Copyright © Trivedi S., Belgamwar V., Wadher K., Umekar M., 20222022Trivedi S., Belgamwar V., Wadher K., Umekar M.Trivedi S., Belgamwar V., Wadher K., Umekar M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1015

Разработан простой УФ-спектрофотометрический метод определения содержания лентинана в комплексе с азокрасителем конго красным при различных концентрациях щелочных растворов. Обнаружено, что лентинан легко растворяется в деионизированной воде с максимумом поглощения при 486 нм. В результате проверки метода выявлено, что раствор комплекса лентинан–конго красный имеет приемлемую линейность в диапазоне концентраций 2–10 мкг/мл с коэффициентом корреляции r2 > 0.99, среднее значение извлечения при различных концентрациях >90 %, для лентинана LOD = 0.014 мкг/мл и LOQ = 0.0431 мкг/мл. Разработанный метод может использоваться для контроля качества при анализе лентинана чистого и в лекарственных препаратах.

Lentinan, a therapeutic bioactive molecule obtained from Lentinus edodes (Shitake mushrooms), possesses various pharmacological activities. A literature survey reveals that no simple UV-visible technique for the estimation of lentinan has yet been established; hence, there is a pressing need for a simple, yet precise and robust analytical method. We have developed a UV-visible spectrophotometric method for the estimation of lentinan in complex with Congo red azo dye in different concentrations of alkaline solutions. Lentinan was found to be freely soluble in deionized water, and the absorption maximum (λmax) was found to be 486 nm. The validation of the developed method gave satisfactory results, the lentinan–Congo red solution complex gave acceptable linearity within the concentration range 2–10 μg/mL and the correlation coefficient (r2) was found to be >0.99. The developed method was found to be accurate because the mean recovery value at various concentrations gave higher results than 90%. LOD and LOQ for lentinan were reported and found to be 0.014 and 0.0431 μg/mL, respectively. The developed method was found to be simple, specific, economic, reliable, accurate, precise, reproducible, and it could be used as a quality control tool for the analysis of pure lentinan and lentinan in formulations.

лентинанконго красныйкомплексообразованиевалидацияУФ-спектроскопияlentinanCongo redcomplex formationvalidationUV spectroscopyThe authors are thankful to the Government of India, Ministry of Science and Technology, Department of Science and Technology (DST), India, for their financial support through the DST-INSPIRE Fellowship (IF 190486) and express their gratitude to Rashtrasant Tukadoji Maharaj Nagpur University Nagpur, Maharashtra, India, for carrying out this project.The authors are thankful to the Government of India, Ministry of Science and Technology, Department of Science and Technology (DST), India, for their financial support through the DST-INSPIRE Fellowship (IF 190486) and express their gratitude to Rashtrasant Tukadoji Maharaj Nagpur University Nagpur, Maharashtra, India, for carrying out this project.ReferencesC. K. Miyaji, A. Poersch, L. R. Ribeiro, A. F. Eira, I. M. S. Cólus, Toxicol. Vitr., 20, 1555–1559 (2006), https://doi.org/10.1016/j.tiv.2006.07.004.C. K. Miyaji, A. Poersch, L. R. Ribeiro, A. F. Eira, I. M. S. Cólus, Toxicol. Vitr., 20, 1555–1559 (2006), https://doi.org/10.1016/j.tiv.2006.07.004.X. Chen, Z. Sheng, S. Qiu, H. Yang, J. Jia, J. Wang, C. Jiang, PLoS One, 14 (2019), https://doi.org/10.1371/journal.pone.0208611.X. Chen, Z. Sheng, S. Qiu, H. Yang, J. Jia, J. Wang, C. Jiang, PLoS One, 14 (2019), https://doi.org/10.1371/journal.pone.0208611.D. Brauer, T. E. Kimmons, M. Phillips, D. E. Brauer, Curr. Res., Technol. and Ed. Top. in Appl. Microbiol. Microbial Biotech., Microbiol., Book Ser., 2, Formatex Res. Center, Badajor, Spain, 1136–1142 (2010).D. Brauer, T. E. Kimmons, M. Phillips, D. E. Brauer, Curr. Res., Technol. and Ed. Top. in Appl. Microbiol. Microbial Biotech., Microbiol., Book Ser., 2, Formatex Res. Center, Badajor, Spain, 1136–1142 (2010).Y. Wang, H. Wang, D. Yan, L. Wang, Z. Sun, H. Sun, Aquac. Int., 21, 1261–1277 (2013), https://doi.org/10.1007/s10499-013-9628-7.Y. Wang, H. Wang, D. Yan, L. Wang, Z. Sun, H. Sun, Aquac. Int., 21, 1261–1277 (2013), https://doi.org/10.1007/s10499-013-9628-7.A. Maruca, F. Moraca, R. Rocca, F. Molisani, F. Alcaro, M. C. Gidaro, S. Alcaro, G. Costa, F. Ortuso, Molecules, 22 (2017), https://doi.org/10.3390/molecules22091571.A. Maruca, F. Moraca, R. Rocca, F. Molisani, F. Alcaro, M. C. Gidaro, S. Alcaro, G. Costa, F. Ortuso, Molecules, 22 (2017), https://doi.org/10.3390/molecules22091571.M. L. Ng, A. T. Yap, J. Altern. Complement. Med., 8, 581–589 (2002), https://doi.org/10.1089/107555302320825093.M. L. Ng, A. T. Yap, J. Altern. Complement. Med., 8, 581–589 (2002), https://doi.org/10.1089/107555302320825093.X. Ma, S. Yuan, L. Yang, L. Li, X. Zhang, C. Su, K. Wang, Cryst. Eng. Commun., 15, 8288–8299 (2013), https://doi.org/10.1039/c3ce41275j.X. Ma, S. Yuan, L. Yang, L. Li, X. Zhang, C. Su, K. Wang, Cryst. Eng. Commun., 15, 8288–8299 (2013), https://doi.org/10.1039/c3ce41275j.N. A. Mohd Jamil, N. Rahmad, N. Mohd Nor Rashid, M. H. Y. Mohd Yusoff, N. S. Shaharuddin, N. Mohd Saleh, J. Mycol., 1–8 (2013), https://doi.org/10.1155/2013/718963.N. A. Mohd Jamil, N. Rahmad, N. Mohd Nor Rashid, M. H. Y. Mohd Yusoff, N. S. Shaharuddin, N. Mohd Saleh, J. Mycol., 1–8 (2013), https://doi.org/10.1155/2013/718963.P. S. Jain, A. J. Chaudhari, S. A. Patel, Z. N. Patel, D. T. Patel, Pharm. Methods, 2, 198–202 (2011), https://doi.org/10.4103/2229-4708.90364.P. S. Jain, A. J. Chaudhari, S. A. Patel, Z. N. Patel, D. T. Patel, Pharm. Methods, 2, 198–202 (2011), https://doi.org/10.4103/2229-4708.90364.H. H. Lee, J. S. Lee, J. Y. Cho, Y. E. Kim, E. K. Hong, J. Microbiol. Biotechnol., 19, 455–461 (2009), https://doi.org/10.4014/jmb.0809.542.H. H. Lee, J. S. Lee, J. Y. Cho, Y. E. Kim, E. K. Hong, J. Microbiol. Biotechnol., 19, 455–461 (2009), https://doi.org/10.4014/jmb.0809.542.K. Ogawa, T. Dohmaru, T. Yui, Biosci. Biotechnol. Biochem., 58, 1870–1872 (1994), https://doi.org/10.1271/bbb.58.1870.K. Ogawa, T. Dohmaru, T. Yui, Biosci. Biotechnol. Biochem., 58, 1870–1872 (1994), https://doi.org/10.1271/bbb.58.1870.M. M. M. Tomassen, E. A. H. J. Hendrix, A. S. M. Sonnenberg, H. J. Wichers, J. J. Mes, Proc. 7th Int. Conf. World Soc. Mushroom Biology and Mushroom Products, Arcachon, France, 04.10.2011, INRA, 259–267 (2011).M. M. M. Tomassen, E. A. H. J. Hendrix, A. S. M. Sonnenberg, H. J. Wichers, J. J. Mes, Proc. 7th Int. Conf. World Soc. Mushroom Biology and Mushroom Products, Arcachon, France, 04.10.2011, INRA, 259–267 (2011).

The authors declare that there are no conflicts of interest present.