zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1021Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESОбнаружение β-лактамных антибиотиков на основе конъюгированных антител с наностержнями золота с помощью спектрометра локализованного поверхностного плазмонного резонансаDetection of β-Lactam Antibiotics Based on Conjugated Antibody with Gold Nanorods by Localized Surface Plasmon Resonance SpectrometerAghamirzaeiМ.AghamirzaeiM.

 Тебриз 

 Фардис, Альборз, Карадж 

 Tabriz 

 Karaj 

aghamirzaei.ma88@gmail.comKhiabaniM. S.KhiabaniM. S.

 Тебриз 

 Tabriz 

HamishehkarH.HamishehkarH.

Тебриз

 Tabriz 

MokarramR. R.MokarramR. R.

 Тебриз 

 Tabriz 

AmjadiM.AmjadiM.

 Тебриз 

 Tabriz 

Тебризский университет; Университет медицинских наук АльборзUniversity of Tabriz; Food and Drug Administration, Alborz University of Medical SciencesТебризский университетUniversity of TabrizЦентр прикладных исследований лекарственных средств при Тебризском университете медицинских наукDrug Applied Research Center at Tabriz University of Medical Sciences202225032022892291291Copyright © Aghamirzaei М., Khiabani M.S., Hamishehkar H., Mokarram R.R., Amjadi M., 20222022Aghamirzaei М., Khiabani M.S., Hamishehkar H., Mokarram R.R., Amjadi M.Aghamirzaei M., Khiabani M.S., Hamishehkar H., Mokarram R.R., Amjadi M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1021

Для определения β-лактамных антибиотиков (ампициллинa, амоксициллинa, пенициллинa G, оксациллинa и карбенициллинa) использованы конъюгированные антитела с наностержнями золота (AuNR). Методы XRD, ATR-FTIR-спектроскопии, TEM и DLS применялись для обнаружения кристалличности, идентификации функциональных групп, участвующих в синтезе AuNR, и для измерения размера AuNR соответственно. Значения pH 9 и концентрации 9.6 мкг антител 1 мл раствора поли(4-стиролсульфоновой кислоты (PSS), модифицированного AuNR, выбраны как лучшие значения pH и концентрации антител для конъюгации с AuNR, модифицированными PSS. Максимумы lmax AuNR, модифицированных PSS, конъюгации антител с AuNR, модифицированных PSS, и обнаружения антибиотиков (от 1 нМ до 1 мМ) с AuNR-PAb, модифицированными PSS, зарегистрированы с помощью микрообъемного спектрофотометра. Результаты показывают, что длина волны поглощения локализованного поверхностного плазмонного резонанса для AuNR, модифицированных PSS, смещена в красную область из-за увеличения концентрации β-лактамных антибиотиков. С увеличением концентрации ампициллина, пенициллина G и карбенициллина положение максимума изменяется и после насыщения антибиотиком кривая выходит на плато, т. е. антитело имеет аналогичное поведение при обнаружении этих антибиотиков. Для амоксициллина концентрация насыщения намного выше, т. е. антитело более специфично для его обнаружения. В случае оксациллина насыщение происходит быстро, демонстрируя чрезвычайно низкую способность антител к обнаружению этого антибиотика. Показано, что антитела чувствительны к концентрации 1 нМ пяти изученных β-лактамных антибиотиков.

This study aimed to determine the β-lactam antibiotics (ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin) using conjugated antibody along with gold nanorods (AuNRs). For this purpose, XRD, ATR-FTIR spectroscopy, transmission electron microscopy, and dynamic light scattering were utilized to detect the crystallinity, to identify functional groups involved in the synthesis of AuNRs, and to measure the size of the AuNRs, respectively. In this regard, pH of 9 and a concentration of 9.6 μg of antibody at 1 mL poly (4-styrenesulfonic acid (PSS))-modified AuNRs solution were selected as the best levels of pH and concentration of antibody for the conjugation of antibody with PSS-modified AuNRs. Thereafter, the maximum wavelength rates of the PSS-modified AuNRs, conjugation of antibody with PSS-modified AuNRs, and detection of antibiotics (from 1 nM to 1 mM) with PSS-modified AuNRs-PAb were recorded using a micro-volume spectrophotometer system. The results indicate that the LSPR absorption wavelength of PSS-modified AuNRs is red-shifted by increasing the concentration of β-lactam antibiotics. By increasing the concentrations of ampicillin, penicillin G, and carbenicillin, the maximum wavelength changed, and after the saturation of the antibiotic concentration, the curve reached a plateau. Correspondingly, this indicated that the antibody had a similar behavior in the detection of these antibiotics. However, regarding amoxicillin, the saturation concentration is much higher, which indicate that the antibody is more specific for its detection. In contrast, for oxacillin, saturation occurred immediately, demonstrating that the antibody had an extremely low detection capability for this antibiotic. Finally, the findings showed that the antibody was sensitive to 1 nM of five studied β-lactam antibiotics.

спектрометр локализованного поверхностного плазмонного резонансаβ-лактамные антибиотикиполиклональные антитела β-лактамананостержни золотараствор поли(4-стиролсульфоновой кислоты)бромид цетилтриметиламмонияконъюгацияlocalized surface plasmon resonance spectrometerβ-lactam antibioticspolyclonal antibody of β-lactamgold nanorodspoly(4-styrenesulfonic acid) solutioncetyltrimethylammonium bromideconjugationThis article is a part of a Ph.D. thesis approved at the University of Tabriz. This work was financially supported by Iran Nanotechnology Initiative Council (Grant code: 136472). The authors would like to thank Prof. Mohammad Javad Rasaee from Tarbiat Modares University; Dr. Seyed Mohammad Amini from Iran University of Medical Sciences; and Mr. Gholamreza Behrouzi, Mr. Babak Farshid, Miss Zahra Abolghasemi-Fakhri, and Mrs. Samira Tizchang for their very useful comments. In addition, we thank Rojan Azma Company and its personnel for providing research equipment. 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The authors declare that there are no conflicts of interest present.