zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1146Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESДВУXМЕРНАЯ И ТРЕХМЕРНАЯ СПЕКТРОСКОПИЯ КОМБИНАЦИОННОГО РАССЕЯНИЯ СВЕТА КЛЕТОК Spirulina platensisTWO-DIMENSIONAL AND THREE-DIMENSIONAL RAMAN SPECTROSCOPIC IMAGING OF Spirulina platensis CELLSZhangS.ZhangS.

Сямэнь, Фуцзянь

Shudi Zhang

Xiamen, Fujian

zsd@xmmc.edu.cnGaoS.GaoS.

Сямэнь, Фуцзянь

Shuitian Gao

Xiamen, Fujian

ChenF.ChenF.

Сямэнь, Фуцзянь

Fangfang Chen

Xiamen, Fujian

PangH.PangH.

Сямэнь, Фуцзянь

Haiyue Pang

Xiamen, Fujian

GaoY.GaoY.

Шэньчжэн, Гуандун

Yanfen Gao

Shenzheng, Guangdong

WangG.WangG.

Сямэнь, Фуцзянь

Gueyhorng Wang

Xiamen, Fujian

Колледж натуральной косметики провинции Фуцзянь; Сямэньский медицинский колледжEngineering Research Center of Natural Cosmeceuticals College of Fujian Province, Xiamen Medical College; Department of Public Health and Medical Technology, Xiamen Medical CollegeШэньчжэньский центр анализа и испытаний пищевых продуктовShenzhen Space Food Analysis and Test Center Co. Ltd202227092022895741741Copyright © Zhang S., Gao S., Chen F., Pang H., Gao Y., Wang G., 20222022Zhang S., Gao S., Chen F., Pang H., Gao Y., Wang G.Zhang S., Gao S., Chen F., Pang H., Gao Y., Wang G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1146

Двухмерная (2D) и трехмерная (3D) спектроскопия комбинационного рассеяния света использована для изучения химического распределения и морфологии клеток водорослей Spirulina platensis. Трихомы Spirulina platensis легко повреждаются лазерным излучением, если параметры лазера не отрегулированы. Спектры комбинационного рассеяния света имеют признаки флуоресценции, которая меняется при кумулятивном лазерном освещении на том же пятне. Из спектров для химического картирования отобраны шесть пиков, характеризующих различные клеточные особенности, включая центральную и периферическую части цитоплазмы, клеточную стенку, оболочку и остатки среды. Обсуждаются возможные химические вещества/органеллы в этих частях и механизмы.

Two-dimensional (2D) and 3D Raman spectroscopic imaging of Spirulina platensis was carried out to investigate its applicability in studying the chemical distribution and morphology of algal cells. The Spirulina trichome was easily damaged by laser illumination unless the laser parameters were properly adjusted. Raman spectra exhibited an evident sign of fluorescence, which changed during cumulative laser illumination on the same spot. Six peaks were selected from the spectra for chemical mapping, which showed distinct cellular features, including the central and peripheral parts of the cytoplasm, cell wall, sheath, and medium residues. Possible chemicals/organelles in these parts and mechanisms were discussed.

Spirulina platensisспектроскопия комбинационного рассеяния светахимическая визуализацияморфология клетокSpirulina platensisRaman spectroscopychemical imagingcell morphologyWe gratefully thank the Educational and Scientific Research Program for Young Scholars Sponsored by the Educational Department of Fujian Province (JAT200733) and the Emerging and Future Industry Development Funds in Shenzhen (20170503163617880) for funding this study. This study was funded by the Educational and Scientific Research Program for Young Scholars Sponsored by the Educational Department of Fujian Province (JAT200733) and the Emerging and Future Industry Development Funds in Shenzhen (20170503163617880).ReferencesA. Kulshreshtha, A. Z. Jarouliya, U. Jarouliya, P. Bhadauriya, G. B. K. S. Prasad, P. S. Bisen, Curr. Pharm. Biotech., 9, 400–405 (2008).A. Kulshreshtha, A. Z. Jarouliya, U. Jarouliya, P. Bhadauriya, G. B. K. S. Prasad, P. S. Bisen, Curr. Pharm. Biotech., 9, 400–405 (2008).Z. Ma, F. Ahmed, B. Yuan, W. Zhang, Trends Food Sci. Tech., 88, 439–444 (2019).Z. Ma, F. Ahmed, B. Yuan, W. Zhang, Trends Food Sci. Tech., 88, 439–444 (2019).R. A. Soni, K. Sudhakar, R. S. Rana, Trends Food Sci. Tech., 69, 157–171 (2017).R. A. Soni, K. Sudhakar, R. S. Rana, Trends Food Sci. Tech., 69, 157–171 (2017).A. Niccolai, G. Chini Zittelli, L. Rodolfi, N. Biondi, M. R. Tredici, Algal Res., 42, 101617 (2019).A. Niccolai, G. Chini Zittelli, L. Rodolfi, N. Biondi, M. R. Tredici, Algal Res., 42, 101617 (2019).H. Qiao, D. Hu, J. Ma, X. Wang, H. Wu, J. Wang, Algal Res., 41, 101540 (2019).H. Qiao, D. Hu, J. Ma, X. Wang, H. Wu, J. Wang, Algal Res., 41, 101540 (2019).A. Rempel, F. de Souza Sossella, A. C. Margarites, A. L. Astolfi, R. L. R. Steinmetz, A. Kunz, H. Treichel, L. M. Colla, Bioresour. Tech., 288, 121588 (2019).A. Rempel, F. de Souza Sossella, A. C. Margarites, A. L. Astolfi, R. L. R. Steinmetz, A. Kunz, H. Treichel, L. M. Colla, Bioresour. Tech., 288, 121588 (2019).H.-M. D. Wang, C.-C. Chen, P. Huynh, J.-S. Chang, Bioresour. Tech., 184, 355–362 (2015).H.-M. D. Wang, C.-C. Chen, P. Huynh, J.-S. Chang, Bioresour. Tech., 184, 355–362 (2015).W. Zhou, Y. Li, Y. Gao, H. Zhao, Bioresour. Tech., 245, 10–17 (2017).W. Zhou, Y. Li, Y. Gao, H. Zhao, Bioresour. Tech., 245, 10–17 (2017).Y. Li, C. Lammi, G. Boschin, A. Arnoldi, G. Aiello, J. Agric. Food Chem., 67, 11825–11838 (2019).Y. Li, C. Lammi, G. Boschin, A. Arnoldi, G. Aiello, J. Agric. Food Chem., 67, 11825–11838 (2019).P. T. Scaglioni, M. Blandino, V. Scarpino, D. Giordano, G. Testa, E. Badiale-Furlong, J. Agric. Food Chem., 66, 4835–4841 (2018).P. T. Scaglioni, M. Blandino, V. Scarpino, D. Giordano, G. Testa, E. Badiale-Furlong, J. Agric. Food Chem., 66, 4835–4841 (2018).G. Usharani, P. Saranraj, D. Kanchana, Int. J Pharm. Biol. Arch., 3, 1327–1341 (2012).G. Usharani, P. Saranraj, D. Kanchana, Int. J Pharm. Biol. Arch., 3, 1327–1341 (2012).X. Cao, Y. Xi, J. Liu, Y. Chu, P. Wu, M. Yang, Z. Chi, S. Xue, Algal Res., 38, 101370 (2019).X. Cao, Y. Xi, J. Liu, Y. Chu, P. Wu, M. Yang, Z. Chi, S. Xue, Algal Res., 38, 101370 (2019).C. Liu, D. Zou, Y. Yang, B. Chen, H. Jiang, J. Appl. Phycol., 29, 983–991 (2017).C. Liu, D. Zou, Y. Yang, B. Chen, H. Jiang, J. Appl. Phycol., 29, 983–991 (2017).S. White, A. Anandraj, F. Bux, Bioresour. Tech., 102, 1675–1682 (2011).S. White, A. Anandraj, F. Bux, Bioresour. Tech., 102, 1675–1682 (2011).C.-J. Kim, Y.-H. Jung, H.-M. Oh, J. Microbiol., 45, 122–127 (2007).C.-J. Kim, Y.-H. Jung, H.-M. Oh, J. Microbiol., 45, 122–127 (2007).S. Rajagopal, C. Sicora, Z. Várkonyi, L. Mustárdy, P. Mohanty, Photosynth. Res., 85, 181–189 (2005).S. Rajagopal, C. Sicora, Z. Várkonyi, L. Mustárdy, P. Mohanty, Photosynth. Res., 85, 181–189 (2005).C. van Eykelenburg, Antonie van Leeuwenhoek, 45, 369–390 (1979).C. van Eykelenburg, Antonie van Leeuwenhoek, 45, 369–390 (1979).C. van Eykelenburg, Antonie van Leeuwenhoek, 46, 113–127 (1980).C. van Eykelenburg, Antonie van Leeuwenhoek, 46, 113–127 (1980).H. Wu, K. Gao, V. E. Villafañe, T. Watanabe, E. W. Helbling, Appl. Environ. Microbiol., 71, 5004–5013 (2005).H. Wu, K. Gao, V. E. Villafañe, T. Watanabe, E. W. Helbling, Appl. Environ. Microbiol., 71, 5004–5013 (2005).A. Braz, M. López-López, C. García-Ruiz, Forens. Sci. Int., 249, 92–100 (2015).A. Braz, M. López-López, C. García-Ruiz, Forens. Sci. Int., 249, 92–100 (2015).J. Bueno, I. K. Lednev, Anal. Bioanal. Chem., 406, 4595–4599 (2014).J. Bueno, I. K. Lednev, Anal. Bioanal. Chem., 406, 4595–4599 (2014).G. Montalvo, L. López-Melero, F. Ortega-Ojeda, M. Á. Peña, C. García-Ruiz, Anal. Methods, 6, 9536–9546 (2014).G. Montalvo, L. López-Melero, F. Ortega-Ojeda, M. Á. Peña, C. García-Ruiz, Anal. Methods, 6, 9536–9546 (2014).T. Yang, B. Zhao, R. Hou, Z. Zhang, A. J. Kinchla, J. M. Clark, L. He, J. Food Sci., 81, 2891–2901 (2016).T. Yang, B. Zhao, R. Hou, Z. Zhang, A. J. Kinchla, J. M. Clark, L. He, J. Food Sci., 81, 2891–2901 (2016).Y. Yang, X. Wang, C. Zhao, G. Tian, H. Zhang, H. Xiao, L. He, J. Zheng, J. Food Sci., 82, 2840–2846 (2017).Y. Yang, X. Wang, C. Zhao, G. Tian, H. Zhang, H. Xiao, L. He, J. Zheng, J. Food Sci., 82, 2840–2846 (2017).C. Kallepitis, M. S. Bergholt, M. M. Mazo, V. Leonardo, S. C. Skaalure, S. A. Maynard, M. M. Stevens, Nat. Commun., 8, 14843 (2017).C. Kallepitis, M. S. Bergholt, M. M. Mazo, V. Leonardo, S. C. Skaalure, S. A. Maynard, M. M. Stevens, Nat. Commun., 8, 14843 (2017).H. Zhang, J. Zheng, A. Liu, H. Xiao, L. He, J. Agric. Food Chem., 64, 9708–9713 (2016).H. Zhang, J. Zheng, A. Liu, H. Xiao, L. He, J. Agric. Food Chem., 64, 9708–9713 (2016).B. Durrant, M. Trappett, D. Shipp, I. Notingher, Curr. Opin. Chem. Biol., 51, 138–145 (2019).B. Durrant, M. Trappett, D. Shipp, I. Notingher, Curr. Opin. Chem. Biol., 51, 138–145 (2019).M. Hosokawa, M. Ando, S. Mukai, K. Osada, T. Yoshino, H.-O. Hamaguchi, T. Tanaka, Anal. Chem., 86, 8224–8230 (2014).M. Hosokawa, M. Ando, S. Mukai, K. Osada, T. Yoshino, H.-O. Hamaguchi, T. Tanaka, Anal. Chem., 86, 8224–8230 (2014).D. T. P. Niranjan, T. Vikas, J. Nanomed. Nanotech., 3, 1000131 (2012).D. T. P. Niranjan, T. Vikas, J. Nanomed. Nanotech., 3, 1000131 (2012).O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, M. Trtílek, Sensors, 10, 8635–8651 (2010).O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, M. Trtílek, Sensors, 10, 8635–8651 (2010).K. T. Schomacker, Y. Domankevitz, T. J. Flotte, T. F. Deutsch, Lasers Surg. Med., 11, 141–151 (1991).K. T. Schomacker, Y. Domankevitz, T. J. Flotte, T. F. Deutsch, Lasers Surg. Med., 11, 141–151 (1991).V. Venugopalan, N. S. Nishioka, B. B. Mikič, J. Biomech. Eng., 116, 62–70 (1994).V. Venugopalan, N. S. Nishioka, B. B. Mikič, J. Biomech. Eng., 116, 62–70 (1994).G. Hedenskog, A. V. Hofsten, Physiol. Plant., 23, 209–216 (1970).G. Hedenskog, A. V. Hofsten, Physiol. Plant., 23, 209–216 (1970).C. Van Eykelenburg, Antonie van Leeuwenhoek, 43, 89–99 (1977).C. Van Eykelenburg, Antonie van Leeuwenhoek, 43, 89–99 (1977).L. Tomaselli, In Spirulina Platensis Arthrospira: Physiology, Cell-Biology and Biotechnology, Taylor & Francis (1997).L. Tomaselli, In Spirulina Platensis Arthrospira: Physiology, Cell-Biology and Biotechnology, Taylor & Francis (1997).

The authors declare that there are no conflicts of interest present.