zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1150Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESАПТАСЕНСОР НА ОСНОВЕ УСИЛЕННОЙ ФЛУОРЕСЦЕНЦИИ ДЛЯ ЧУВСТВИТЕЛЬНОГО ДЕТЕКТИРОВАНИЯ ИОНОВ СЕРЕБРАDESIGN OF A FLUORESCENCE ENHANCED APTASENSOR FOR SENSITIVE DETECTION OF SILVER IONSRenL.RenL.

Чжэнчжоу

Linjiao Ren

Zhengzhou

ChenG.ChenG.

Чжэнчжоу

Gaokai Chen

Zhengzhou

PengZ.PengZ.

Чжэнчжоу

Zheng Peng

Zhengzhou

XuX.XuX.

Чжэнчжоу

Xiaoping Xu

Zhengzhou

ZhangP.ZhangP.

Чжэнчжоу

Pei Zhang

Zhengzhou

QinZ.QinZ.

Чжэнчжоу

Zirui Qin

Zhengzhou

ChenQ.ChenQ.

Чжэнчжоу

Qinghua Chen

Zhengzhou

YanY.YanY.

Чжэнчжоу

Yanxia Yan

Zhengzhou

JiangL.JiangL.

Чжэнчжоу

Liying Jiang

Zhengzhou

jiangliying@zzuli.edu.cnШкола электротехники и информационной инженерии Чжэнчжоуского университета легкой промышленностиSchool of Electrical and Information Engineering at Zhengzhou University of Light Industry202227092022895745745Copyright © Ren L., Chen G., Peng Z., Xu X., Zhang P., Qin Z., Chen Q., Yan Y., Jiang L., 20222022Ren L., Chen G., Peng Z., Xu X., Zhang P., Qin Z., Chen Q., Yan Y., Jiang L.Ren L., Chen G., Peng Z., Xu X., Zhang P., Qin Z., Chen Q., Yan Y., Jiang L.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1150

Разработан высокочувствительный аптасенсор на основе усиленной флуоресценции металла для обнаружения ионов серебра Ag+. Взаимодействие Ag+ с азотистыми основаниями цитозина использовано для достижения низкого предела обнаружения. Модифицированные аптамером наночастицы золота (НЧ Au) смешивали с ДНК, меченной 6-карбо-ксифлуоресцеином (FAM), для приготовления сенсора. В растворе без Ag+ аптамер и меченные FAM нити ДНК оставались свободными. В присутствии Ag+ пары нитей ДНК, меченных аптамером и FAM, образовывали двойные спирали за счет взаимодействий цитозин–Аg+–цитозин, которые привели к уменьшению расстояния между FAM и НЧ Au. Количество адениновых повторов в аптамере изменялось с целью регулирования расстояния между НЧ Au и FAM и обеспечения контролируемого локализованного поверхностного плазмонного резонанса. При оптимальном количестве адениновых повторов (n = 24) линейный диапазон обнаружения Ag+ 0.694–6.94 нмоль/л, предел обнаружения 0.694 нмоль/л. Аптасенсор показал превосходную специфичность и позволил обнаружить ионы серебра, присутствующего в следовых концентрациях

A highly sensitive fluorescence-enhanced aptasensor was designed to detect silver ions (Ag+) using metal-enhanced fluorescence. Interaction of Ag+ with cytosine nucleobases was used to achieve a low detection limit. Aptamer-modified gold nanoparticles (Au NPs) were mixed with 6-carboxyfluorescein (FAM)-labeled DNA to prepare the sensor. In a solution without Ag+, aptamer and FAM-labeled DNA strands remained free because of repulsion between cytosine. In the presence of Ag+, pairs of aptamer and FAM-labeled DNA strands formed double helices through cytosine–Ag+–cytosine interactions. These interactions brought FAM close to the Au NPs. The number of adenines repeats in the aptamer was altered to adjust the distance between the Au NPs and FAM, and provide controllable localized surface plasmon resonance. With the optimum number of adenine repeats (n = 24), the linear range for detection of Ag+ was 0.694 to 6.94 nmol/L and the detection limit was 0.694 nmol/L. The aptasensor showed excellent specificity and gave a strong detection signal for Ag+ present at trace concentrations to overcome issues associated with the detection of weak signals.

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The authors declare that there are no conflicts of interest present.