zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1175Research ArticleСтатьи400 МГц ЯМР-профили метаболитов кист челюсти, абсцессов челюсти, слюны и урины, полученных путем разбавления образцов D2O400 MHz Nuclear Magnetic Resonance Metabolite Profiles of Jaw Cysts, Jaw Abscesses, Saliva, and Urine Obtained by Massive D2O Dilution of SamplesYilmazU. N.YilmazU. N.

Отделение челюстно-лицевой хирургии стоматологического факультета, 

Диярбакыр

Department of Oral-Maxillofacial Surgery, Faculty of Dentistry, 

Diyarbakır

utkunezih@gmail.comYilmazB. D.YilmazB. D.

Отделение протезирования стоматологического факультета, 

Диярбакыр

Department of Prosthodontics, Faculty of Dentistry, 

Diyarbakır

KöylüM. Z.KöylüM. Z.

Отделение физики, 

Диярбакыр

Department of Physics, Faculty of Science, 

Diyarbakır

Университет ДиклеUniversity of DicleУниверситет ТиграUniversity of Dicle202230112022896845851Copyright © Yilmaz U.N., Yilmaz B.D., Köylü M.Z., 20222022Yilmaz U.N., Yilmaz B.D., Köylü M.Z.Yilmaz U.N., Yilmaz B.D., Köylü M.Z.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1175

Профили метаболитов кист челюсти, абсцессов челюсти, слюны и мочи, полученных при значительном разбавлении D2O, исследованы с помощью магнитно-резонансной спектроскопии (МРС) без использования методов подавления воды. Образцы готовили путем добавления 0.06 мл каждой биожидкости к 0.94 мл. Одноимпульсные эксперименты проводились с использованием спектрометра ядерного магнитного резонанса (ЯМР) с частотой 400 МГц. Профили метаболитов с высоким разрешением получены для большинства абсцессов челюсти, некоторых кист челюсти и всех образцов слюны и урины. За исключением одной кисты челюсти, для остальных образцов обнаружена широкая огибающая спектров, содержащая различные метаболиты. Однако спектры высокого разрешения для этих образцов кистозной жидкости получены при 40%-ном разбавлении H2O. Представленный подход не требует подавления воды и использует относительно низкочастотный ЯМР, а также позволяет устранить демпфирование излучения, сократить время эксперимента и проводить измерения релаксации. 

The metabolite profiles of various cysts and abscesses outside the jaw area have been investigated by magnetic resonance spectroscopy (MRS) using water-suppression techniques. However, metabolite profiles of jaw cysts, jaw abscesses, saliva, and urine have not yet been obtained by MRS using massive D2O dilution of samples. This study was aimed at obtaining metabolite profiles of these bio-fluids by MRS without water suppression. In total, 37 jaw cysts, 19 jaw abscesses, 10 saliva and 10 urine samples were collected. Dilutions were prepared by adding 0.06 mL of each sample to 0.94 mL D2O. Single-pulse experiments were performed using a 400 MHz nuclear magnetic resonance (NMR) spectrometer. High-resolution metabolite profiles were obtained for most jaw abscesses (13), some jaw cysts (8), all saliva, and all urine samples. Except for one jaw cyst, the remaining samples showed a broad envelope containing various metabolites. However, high-resolution spectra for these cystic fluid samples were obtained through 40% dilution with H2O. In conclusion, to the best of our knowledge, metabolite profiles of jaw cysts, abscesses, saliva, and urine by massive D2O dilution were first presented here. The presented approach does not require water suppression and uses a relatively low frequency NMR. It is also a simple and novel method of eliminating radiation damping, reducing experimental time, and allowing relaxation measurements. 

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The authors declare that there are no conflicts of interest present.