zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1226Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESСпектроскопический анализ взаимодействия ионов цинка с пероксидазой хренаSpectroscopic Analysis of the Zinc Ion Interaction with Horseradish PeroxidaseShiraziN. H.ShiraziN. H.

Рудехен

Roudehen

na.hadizadeh@iau.ac.irRajabiM. R.RajabiM. R.

Рудехен

Roudehen

Рудехенский филиал Исламского университета АзадDepartment of Biology, Roudehen Branch, Islamic Azad University202304022023901112112Copyright © Shirazi N.H., Rajabi M.R., 20232023Shirazi N.H., Rajabi M.R.Shirazi N.H., Rajabi M.R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1226

С помощью УФ-видимой, флуоресцентной, ИК-Фурье-спектроскопии и кругового дихроизма проанализировано взаимодействие ионов цинка и пероксидазы хрена (HRP). Анализ конформации HRP выявил заметное уменьшение α-спирали с 47% для свободного фермента до 17% для HRP–Zn2+ и уменьшение третичных структур фермента за счет взаимодействия с Zn2+. Интенсивность флуоресценции HRP значительно снижается вследствие наличия Zn2+. Линейная зависимость интенсивности излучения HRP от концентрации ингибитора показала, что процесс вымирания является линейным и происходит равномерно с увеличением концентрации Zn2+. Для взаимодействия цинка и HRP Kq » 1011 л/моль. Это означает, что флуоресценция HRP подавляется Zn2+ с помощью механизма статического тушения. 

The purpose of the current study was to evaluate the interaction between zinc ions and horseradish peroxidase (HRP) by ultraviolet-visible, fluorescence, circular dichroism, and FTIR spectroscopies. HRP conformation analysis revealed a noticeable decrease in α-helix from 47% for the free enzyme to 17% for HRP–Zn2+ and a reduction in tertiary structures of the enzyme through a Zn2+ interaction. Moreover, the fluorescence intensity of HRP was decreased significantly by Zn2+. The linear relationship of HRP emission data and inhibitor concentration indicated that the extinction process is linear and occurs uniformly with increasing Zn2+ concentration. The values of Kq for the zinc and HRP interaction were in the order of 1011 l/mol, which means that HRP fluorescence was quenched by Zn2+ through a static quenching mechanism. 

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The authors declare that there are no conflicts of interest present.