zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1242Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESФлуоресцентные исследования флавоноидных дигликозидов и дегликозилированных моногликозидовFluorescence Studies on Flavonoid Diglycosides and Corresponding Deglycosylated MonoglycosidesLiH.LiH.

Сямынь

Xiamen

LiW.LiW.

Сямынь

Xiamen

HuangG.HuangG.

Сямынь

Xiamen

LiL.LiL.

Сямынь

Xiamen

ljli@jmu.edu.cnNiH.NiH.

Сямынь

Xiamen

Колледж океанической пищевой и биологической инженерии Университета ЦзимэйCollege of Ocean Food and Biological Engineering at Jimei University202304022023901128128Copyright © Li H., Li W., Huang G., Li L., Ni H., 20232023Li H., Li W., Huang G., Li L., Ni H.Li H., Li W., Huang G., Li L., Ni H.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1242

Разработан метод количественного определения флуоресценции, подходящий для высокопроизводительного скрининга каталитической активности рамнозидазы в отношении нарингина. Обнаружение прунина при λex/λem = 360/450 нм основано на различии флуоресценции после нагревания в щелочных условиях, обнаружение нарингина при λex/λem = 325/400 нм — на различии флуоресценции в фосфатном буфере. Предлагаемый метод подходит для предварительного отбора образцов и позволяет обнаруживать флуоресценцию гесперидина и гесперитин-7-о-глюкозида, что обеспечивает эталон для быстрого определения дигликозидов и соответствующих дегликозилированных моногликозидов. 

A fluorescence quantitative detection method suitable for high-throughput screening of rhamnosidase catalytic activity on naringin was established. Detection of prunin at λex/λem = 360/450 nm was based on the fluorescence difference after heating under alkaline conditions. Detection of naringin at λex/λem = 325/400 nm was based on the difference in fluorescence in phosphate buffer. The method is suitable for prescreening many samples. A similar method can detect the fluorescence of hesperidin and hesperitin-7-o-glucoside, which provides a reference for the rapid detection of diglycosides and corresponding deglycosylated monoglycosides. 

флуоресценциянарингинпруниндигликозидмоногликозидfluorescencenaringinprunindiglycosidemonoglycosideWe greatly appreciate useful discussion from Qi Chen, Sun Jiang, and Zhang Yichun, appreciate the funding support from the National Natural Science Foundation of China (No. U1805235), the Science and technology project of Xiamen City, Fujian Province, China (No. 3502Z20203058), and the Central financial award project of the modern agricultural industrial park in Pinghe County, Fujian Province, China (No. PH2020) for this project.ReferencesA. N. Panche, A. D. Diwan, S. R. Chandra, J. Nutr. Sci., 5, e47 (2016).A. N. Panche, A. D. Diwan, S. R. Chandra, J. Nutr. Sci., 5, e47 (2016).T. Wang, Q. Li, K. Bi, Asian J. Pharm. Sci., 13, 12–23 (2018).T. Wang, Q. Li, K. Bi, Asian J. Pharm. Sci., 13, 12–23 (2018).J. Krych, L. Gebicka, Int. J. Biol. Macromol., 58, 148–153 (2013).J. Krych, L. Gebicka, Int. J. Biol. Macromol., 58, 148–153 (2013).F. A. Ragab, T. A. A. Yahya, M. M. El-Naa, R. K. Arafa, Eur. J. Med. Chem., 82, 506–520 (2014).F. A. Ragab, T. A. A. Yahya, M. M. El-Naa, R. K. Arafa, Eur. J. Med. Chem., 82, 506–520 (2014).X. Zhang, H. Huang, X. Zhao, Q. Lv, C. Sun, X. Li, K. Chen, J. Funct. Foods, 14, 144–153 (2015).X. Zhang, H. Huang, X. Zhao, Q. Lv, C. Sun, X. Li, K. Chen, J. Funct. Foods, 14, 144–153 (2015).S.-S. Tian, F.-S. Jiang, K. Zhang, X.-X. Zhu, B. Jin, J.-J. Lu, Z.-S. Ding, Fitoterapia, 92, 34–40 (2014).S.-S. Tian, F.-S. Jiang, K. Zhang, X.-X. Zhu, B. Jin, J.-J. Lu, Z.-S. Ding, Fitoterapia, 92, 34–40 (2014).L. Pourcel, J. Routaboul, V. Cheynier, L. Lepiniec, I. Debeaujon, Trends Plant Sci., 12, 29–36 (2007).L. Pourcel, J. Routaboul, V. Cheynier, L. Lepiniec, I. Debeaujon, Trends Plant Sci., 12, 29–36 (2007).J. Xiao, E. Capanoglu, A. R. Jassbi, A. Miron, Critical Rev. Food Sci. Nutr., 56, S29–S45 (2016).J. Xiao, E. Capanoglu, A. R. Jassbi, A. Miron, Critical Rev. Food Sci. Nutr., 56, S29–S45 (2016).J. Viskupi, M. Ondrejovi, E. Šturdík, J. Food Nutr. Res., 47, 151–162 (2008).J. Viskupi, M. Ondrejovi, E. Šturdík, J. Food Nutr. Res., 47, 151–162 (2008).Y. Li, T. Zhang, G. Chen, Antioxidants, 7, 187 (2018). 11. X.-Y. Zhang, Master Dissertation, Fluorescence Properties and Analytical Methods of Several Isoflavones, Hebei Normal University (2018).Y. Li, T. Zhang, G. Chen, Antioxidants, 7, 187 (2018). 11. X.-Y. Zhang, Master Dissertation, Fluorescence Properties and Analytical Methods of Several Isoflavones, Hebei Normal University (2018).E.Corradini, P.Foglia, P.Giansanti, R.Gubbiotti, R.Samperi,A. Laganà, Nat. Prod. Res., 25, 469–495 (2011).E.Corradini, P.Foglia, P.Giansanti, R.Gubbiotti, R.Samperi,A. Laganà, Nat. Prod. Res., 25, 469–495 (2011).A. Pirogov, L. Sokolova, E. Sokerina, O. Tataurova, O. Shpigun, J. Liq. Chromatogr. Related Tech., 39, 220–224 (2016).A. Pirogov, L. Sokolova, E. Sokerina, O. Tataurova, O. Shpigun, J. Liq. Chromatogr. Related Tech., 39, 220–224 (2016).S. Barnes, M. Kirk, L. Coward, J. Agric. Food Chem., 42, 2466–2474 (1994).S. Barnes, M. Kirk, L. Coward, J. Agric. Food Chem., 42, 2466–2474 (1994).P. Mattila, J. Astola, J. Kumpulainen, J. Agric. Food Chem., 48, 5834–5841 (2000).P. Mattila, J. Astola, J. Kumpulainen, J. Agric. Food Chem., 48, 5834–5841 (2000).R. M. P. Siloto, R. J. Weselake, Biocatal. Agric. Biotech., 1, 181–189 (2012).R. M. P. Siloto, R. J. Weselake, Biocatal. Agric. Biotech., 1, 181–189 (2012).W.-H. Li, Doctoral Dissertation, Study on Fluorescence Properties and Analytical Methods of Flavonoids, Hebei Normal University (2016).W.-H. Li, Doctoral Dissertation, Study on Fluorescence Properties and Analytical Methods of Flavonoids, Hebei Normal University (2016).W.-H. Li, Y.-J. Wei, J.-J. Cao, R. Lu, C.-X. Che, Spectrosc. Spectr. Analysis, 36, 1007–1012 (2016).W.-H. Li, Y.-J. Wei, J.-J. Cao, R. Lu, C.-X. Che, Spectrosc. Spectr. Analysis, 36, 1007–1012 (2016).D.-Y. Wang, Master Dissertation, Study on the Fluorescence Properties of Some Flavonoids and the Fluorescence Analysis Method of Total Isoflavones in Tempeh, Hebei Normal University (2019).D.-Y. Wang, Master Dissertation, Study on the Fluorescence Properties of Some Flavonoids and the Fluorescence Analysis Method of Total Isoflavones in Tempeh, Hebei Normal University (2019).R. Chen, Q.-L. Qi, M.-T. Wang, Q.-Y. Li, Pharm. Biol., 54, 3203–3210 (2016).R. Chen, Q.-L. Qi, M.-T. Wang, Q.-Y. Li, Pharm. Biol., 54, 3203–3210 (2016).H. Vila-Real, A. J. Alfaia, M. R. Bronze, A. R. T. Calado, M. H. L. Ribeiro, Enzyme Res., 1–11 (2011).H. Vila-Real, A. J. Alfaia, M. R. Bronze, A. R. T. Calado, M. H. L. Ribeiro, Enzyme Res., 1–11 (2011).S. Bharti, N. Rani, B. Krishnamurthy, D. Arya, Planta Med., 80, 437–451 (2014).S. Bharti, N. Rani, B. Krishnamurthy, D. Arya, Planta Med., 80, 437–451 (2014).P. Lavrador, V. M. Gaspar, J. F. Mano, Drug Disc. Today, 23, 1293–1304 (2018).P. Lavrador, V. M. Gaspar, J. F. Mano, Drug Disc. Today, 23, 1293–1304 (2018).S. Gunaseelan, K. Z. Wong, N. Min, J. Sun, N. K. B. M. Ismail, Y. J. Tan, R. C. H. Lee, J. J. H. Chu, Sci. Transl. Med., 11, eaar5759 (2019).S. Gunaseelan, K. Z. Wong, N. Min, J. Sun, N. K. B. M. Ismail, Y. J. Tan, R. C. H. Lee, J. J. H. Chu, Sci. Transl. Med., 11, eaar5759 (2019).H. Vilareal, A. Alfaia, A. Calado, M. Ribeiro, Food Chem., 102, 565–570 (2007).H. Vilareal, A. Alfaia, A. Calado, M. Ribeiro, Food Chem., 102, 565–570 (2007).G. Şekeroğlu, S. Fadıloğlu, F. Göğüş, Eur. Food Res. Technol., 224, 55–60 (2006).G. Şekeroğlu, S. Fadıloğlu, F. Göğüş, Eur. Food Res. Technol., 224, 55–60 (2006).

The authors declare that there are no conflicts of interest present.