zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1285Research Article******Экспериментальное обнаружение шести витаминов с помощью лазерно-индуцированной флуоресценцииDetection Experiment of Six Vitamins by Laser-Induced Fluorescence SpectraHuangJ.HuangJing

Сиань

Xi’an

ZhangP.ZhangPeng

Сиань

Xi’an

276877634@qq.comПервая больница при Сианьском университете ЦзяотунDepartment of Rheumatism and Immunology, The First Affiliated Hospital Xi’an Jiaotong UniversityШкола энергетики и электромеханики Чанъаньского университетаSchool of Energy and Electromechanical Engineering at Chang’an University2023260320239023539Copyright © Huang J., Zhang P., 20232023Huang J., Zhang P.Huang J., Zhang P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1285

Cпектры лазерно-индуцированной флуоресценции (LIFS) витаминов A, B1, C, D, E и K1 и их смесей исследованы при возбуждении излучением Nd:YAG-лазера с длиной волны 266 нм. Показано, что LIFS раствора каждого витамина имеет свой уникальный профиль. Витамины различаются по характерным диапазонам длин волн: 320–380 нм для витаминов А и С, 350–440 нм для витаминов D и Е, 400–470 нм для витаминов В1 и K1. Это можно использовать в качестве дополнения для точной диагностики по характеристикам LIFS (начальной длине волны, спектральному диапазону, длине волны и интенсивности максимума, коэффициенту экстинкции). Особенности LIFS могут в определенной степени характеризовать основные компоненты в смеси витаминов. По спектральному диапазону, максимальной интенсивности и коэффициенту экстинкции можно определять количество бензольных колец, двойных связей и групп ОН в молекулах компонентов в смесях, по которым различаются витамины. Разработан метод количественной  идентификации  отдельных  компонентов в смесях с использованием параметров LIFS, а также предложена техническая поддержка в клинической диагностике и таргетной терапии с использованием витаминов.

Laser-induced fluorescence (LIF) is a potential technology for the rapid, sensitive, and selective detection of vitamins. In this study, the LIF spectra (LIFS) of vitamins A, B1, C, D, E, and K1, and their mixtures were investigated under a 266-nm laser excitation via an Nd:YAG laser. The experimental results showed that the LIFS of each vitamin solution had its unique profile. The six vitamins can be distinguished to some extent by the characteristic wavelength region: 320–380 nm for vitamins A and C, 350–440 nm for vitamins D and E, and 400–470 nm for vitamins B1 and K1. Additionally, it can be used for accurate diagnosis by characteristics of LIFS (starting wavelength, spectral range, peak wavelength, maximum intensity, and extinction coefficient) as a complement. Moreover, the features of LIFS can reflect the main vitamin components in the mixture of vitamins to a certain extent when several vitamins coexist. It was found that the spectral range, maximum intensity, and extinction coefficient could report the benzene ring number, double bonds, and OH group in molecules of components in mixtures, which is a discrimination-assisted method for vitamins in a mixture. This paper also proposes a technique to identify individual components in mixtures quantitatively by using LIFS and LIFS parameters. The present study will offer technical support in clinical diagnosis and targeted therapy using vitamins via experimental exploration on detection.

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The authors declare that there are no conflicts of interest present.