zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1496Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESСпектрофотометрическое исследование гидрокортизона в УФ-видимой областиHydrocortisone UV-Vis Spectrophotometric Study: Stability, Determination of Its Acidity Constants and QuantificationRamos-ReyesG. M.Ramos-ReyesG. M.Corona-AvendañoS.Corona-AvendañoS.scav@azc.uam.mxRamírez-SilvaM.T.Ramírez-SilvaM.T.mtrs@xanum.uam.mxRomero-RomoM.Romero-RomoM.Palomar-PardavéM.Palomar-PardavéM.Университет АскапоцалькоUniversidad Autónoma Metropolitana-AzcapotzalcoУниверситет ИстапалапаUniversidad Autónoma Metropolitana-Iztapalapa202414022024911168168Copyright © Ramos-Reyes G.M., Corona-Avendaño S., Ramírez-Silva M., Romero-Romo M., Palomar-Pardavé M., 20242024Ramos-Reyes G.M., Corona-Avendaño S., Ramírez-Silva M., Romero-Romo M., Palomar-Pardavé M.Ramos-Reyes G.M., Corona-Avendaño S., Ramírez-Silva M., Romero-Romo M., Palomar-Pardavé M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1496

Разработан метод спектрофотометрической оценки в УФ-видимом диапазоне химической стабильности гидрокортизона в водной среде в зависимости от времени анализа, определены его константы кислотности с помощью надежной методологии с использованием программного обеспечения SQUAD. Определена константа кислотности pKa 11.45 ± 0.02 (298 К). Молярные коэффициенты поглощения связали с химическими равновесиями, при которых стало возможным предложить механизм депротонирования. Для разработанного аналитического метода пределы обнаружения и количественного определения гидрокортизона составили 386 ± 3 и 1280 ± 10 нМ, валидация показала возможность надежного количественного определения гидрокортизона в фармацевтических препаратах.

Hydrocortisone is a glucocorticoid-type hormone produced naturally by the organism, such that it maintains the body functional during physical or mental stress episodes. The present work focuses on the UV-Vis spectrophotometric assessment of the chemical stability of hydrocortisone in an aqueous medium during the time of analysis, determination of its acidity constants through a robust methodology that involves the usage of SQUAD software and validation of an analytical method to determine hydrocortisone by means of UV-Vis spectrophotometry, in pharmaceuticals. The acidity constant was determined as pKa 11.45 ± 0.02 (298 K), and the molar absorptivity coefficients and the molar absorptivity coefficients of the species involved in this acidity equilibrium were also determined as a function of the wavelength. The analytical method developed attained detection and quantification limits of 386 ± 3 and 1280 ± 10 nM, respectively, whereas the validation allows reliable quantification of hydrocortisone in pharmaceuticals.

гидрокортизонспектрофотометрическое титрованиеконстанта кислотностипрограммное обеспечение SQUADhydrocortisonespectrophotometric titrationacidity constantsSQUAD softwareSCA, MTRS, MRR, and MPP wish to thank the SNI for the distinction of their membership. M. T. Ramírez-Silva thanks DCBI (UAM-Iztapalapa) for the sabbatical year granted. The authors wish to acknowledge the financial support by CONACyT through project 237327. GMRR (693797) expresses his gratitude to Universidad Autónoma Metropolitana and CONACyT for the student grants received to pursue his Ph.D. degree. SCA, MTRS, MRR and MPP wish to thank the SNI for the stipend received.ReferencesK. A. Connors, G. L. Amidon, V. J. Stella, Chemical Stability of Pharmaceuticals: A Handbook for Pharmacists, 2nd ed., John Wiley & Sons, Ney York (1986), https://doi.org/10.1016/S0003-0465(15)31395-1.K. A. Connors, G. L. Amidon, V. J. Stella, Chemical Stability of Pharmaceuticals: A Handbook for Pharmacists, 2nd ed., John Wiley & Sons, Ney York (1986), https://doi.org/10.1016/S0003-0465(15)31395-1.M. Jia, W. M. Chew, Y. Feinstein, P. Skeath, E. M. Sternberg, Analyst, 141, 2053–2060 (2016), https://doi.org/10.1039/c5an02387d.M. Jia, W. M. Chew, Y. Feinstein, P. Skeath, E. M. Sternberg, Analyst, 141, 2053–2060 (2016), https://doi.org/10.1039/c5an02387d.A. Singh, A. Kaushik, R. Kumar, M. Nair, S. Bhansali, Appl. Biochem. Biotechnol., 174, 1115–1126 (2014), https://doi.org/10.1007/s12010-014-0894-2.A. Singh, A. Kaushik, R. Kumar, M. Nair, S. Bhansali, Appl. Biochem. Biotechnol., 174, 1115–1126 (2014), https://doi.org/10.1007/s12010-014-0894-2.A. Kaushik, A. Vasudev, S. K. Arya, S. K. Pasha, S. Bhansali, Biosens. Bioelectron., 53, 499–512 (2014), https://doi.org/10.1016/j.bios.2013.09.060.A. Kaushik, A. Vasudev, S. K. Arya, S. K. Pasha, S. Bhansali, Biosens. Bioelectron., 53, 499–512 (2014), https://doi.org/10.1016/j.bios.2013.09.060.A. I. Lauerma, S. Reitamo, H. I. Maibach, J. Am. Acad. Dermatol., 24, 182–185 (1991), https://doi.org/10.1016/0190-9622(91)70024-V.A. I. Lauerma, S. Reitamo, H. I. Maibach, J. Am. Acad. Dermatol., 24, 182–185 (1991), https://doi.org/10.1016/0190-9622(91)70024-V.A. Machanda, M. Laracy, R. Bogner, Int. J. Pharm. Compd., 22, 66–75 (2018).A. Machanda, M. Laracy, R. Bogner, Int. J. Pharm. Compd., 22, 66–75 (2018).W. R. Lovallo, J. L. Robinson, D. C. Glahn, P. T. Fox, Psychoneuroendocrinology, 35, 15–20 (2010), https://doi.org/10.1016/j.psyneuen.2009.09.010.W. R. Lovallo, J. L. Robinson, D. C. Glahn, P. T. Fox, Psychoneuroendocrinology, 35, 15–20 (2010), https://doi.org/10.1016/j.psyneuen.2009.09.010.S. Kuhlmann, C. Kirschbaum, O. T. Wolf, Neurobiol. Learn Mem., 83, 158–162 (2005), https://doi.org/10.1016/j.nlm.2004.09.001.S. Kuhlmann, C. Kirschbaum, O. T. Wolf, Neurobiol. Learn Mem., 83, 158–162 (2005), https://doi.org/10.1016/j.nlm.2004.09.001.M. Vythilingam, E. Vermetten, G. M. Anderson, D. Luckenbaugh, E. R. Anderson, J. Snow, L. H. Staib, D. S. Charney, J. D. Bremner, Biol. Psychiatry, 56, 101–112 (2004), https://doi.org/10.1016/j.biopsych.2004.04.002.M. Vythilingam, E. Vermetten, G. M. Anderson, D. Luckenbaugh, E. R. Anderson, J. Snow, L. H. Staib, D. S. Charney, J. D. Bremner, Biol. Psychiatry, 56, 101–112 (2004), https://doi.org/10.1016/j.biopsych.2004.04.002.J. Hansen, H. Bundgaalw, Int. J. Pharm., 6, 307 (1980), https://doi.org/10.1016/0378-5173(80)90114-3.J. Hansen, H. Bundgaalw, Int. J. Pharm., 6, 307 (1980), https://doi.org/10.1016/0378-5173(80)90114-3.A. B. Sarkar, R. Dudley, S. Melethil, J. Speidel, G. M. Bhatt, Innov. Pharm., 2, 3–5 (2011), https://doi.org/10.24926/iip.v2i3.237.A. B. Sarkar, R. Dudley, S. Melethil, J. Speidel, G. M. Bhatt, Innov. Pharm., 2, 3–5 (2011), https://doi.org/10.24926/iip.v2i3.237.J. P. Fawcett, D. W. Boulton, R. Jiang, D. J. Woods, Ann. Pharm., 29, 987–990 (1995), https://doi.org/10.1016/S0022-3476(71)80178-6.J. P. Fawcett, D. W. Boulton, R. Jiang, D. J. Woods, Ann. Pharm., 29, 987–990 (1995), https://doi.org/10.1016/S0022-3476(71)80178-6.T. Ghafourian, P. Zandasrar, H. Hamishekar, A. Nokhodchi, J. Controll. Rel., 99, 113–125 (2004), https://doi.org/10.1016/j.jconrel.2004.06.010.T. Ghafourian, P. Zandasrar, H. Hamishekar, A. Nokhodchi, J. Controll. Rel., 99, 113–125 (2004), https://doi.org/10.1016/j.jconrel.2004.06.010.K. Sarafoglou, M. T. Gonzalez-Bolanos, C. L. Zimmerman, T. Boonstra, Y. Addo, R. Brundage, J. Clin. Pharm., 1–5 (2014), https://doi.org/10.1002/jcph.424.K. Sarafoglou, M. T. Gonzalez-Bolanos, C. L. Zimmerman, T. Boonstra, Y. Addo, R. Brundage, J. Clin. Pharm., 1–5 (2014), https://doi.org/10.1002/jcph.424.L. V. Rao, J. R. Petersen, M. G. Bissell, A. O. Okorodudu, A. A. Mohammad, J. Chromatogr. B: Biomed. Sci. Appl., 730, 123–128 (1999), https://doi.org/10.1016/S0378-4347(99)00151-6.L. V. Rao, J. R. Petersen, M. G. Bissell, A. O. Okorodudu, A. A. Mohammad, J. Chromatogr. B: Biomed. Sci. Appl., 730, 123–128 (1999), https://doi.org/10.1016/S0378-4347(99)00151-6.E. Tu, P. Pearlmutter, M. Tiangco, G. Derose, L. Begdache, A. Koh, ACS Omega, 5, 8211–8218 (2020), https://doi.org/10.1021/acsomega.0c00498.E. Tu, P. Pearlmutter, M. Tiangco, G. Derose, L. Begdache, A. Koh, ACS Omega, 5, 8211–8218 (2020), https://doi.org/10.1021/acsomega.0c00498.K. Balaji, G. V. R. Reddy, T. M. Reddy, S. J. Reddy, Afr. J. Pharm. Pharmacol., 2, 157–166 (2008), http://www.academicjournals.org/ajpp.K. Balaji, G. V. R. Reddy, T. M. Reddy, S. J. Reddy, Afr. J. Pharm. Pharmacol., 2, 157–166 (2008), http://www.academicjournals.org/ajpp.J. M. Lemus Gallego, J. Pérez Arroyo, Anal. Chim. Acta, 460, 85–97 (2002), https://doi.org/10.1016/S0003-2670(02)00138-1.J. M. Lemus Gallego, J. Pérez Arroyo, Anal. Chim. Acta, 460, 85–97 (2002), https://doi.org/10.1016/S0003-2670(02)00138-1.S. Dalirirad, A. J. Steckl, Sens. Actuators B Chem., 283, 79–86 (2019), https://doi.org/10.1016/j.snb.2018.11.161.S. Dalirirad, A. J. Steckl, Sens. Actuators B Chem., 283, 79–86 (2019), https://doi.org/10.1016/j.snb.2018.11.161.J. Smajdor, R. Piech, M. Rumin, B. Paczosa-Bator, Electrochim. Acta, 182, 67–72 (2015), https://doi.org/10.1016/j.electacta.2015.09.057.J. Smajdor, R. Piech, M. Rumin, B. Paczosa-Bator, Electrochim. Acta, 182, 67–72 (2015), https://doi.org/10.1016/j.electacta.2015.09.057.R. N. Goyal, S. Chatterjee, A. R. S. Rana, Talanta, 83, 149–155 (2010), https://doi.org/10.1016/j.talanta.2010.08.054.R. N. Goyal, S. Chatterjee, A. R. S. Rana, Talanta, 83, 149–155 (2010), https://doi.org/10.1016/j.talanta.2010.08.054.D. Jenkins, C. Diallo, E. Bethea, E. Kaale, T. Layloff, In: Calibration and Validation of Analytical Methods – A Sampling of Current Approaches, Ed. M. Stauffer, 1st ed., Intech Open, London, United Kingdom, 143–163 (2018), https://doi.org/10.5772/intechopen.71765.D. Jenkins, C. Diallo, E. Bethea, E. Kaale, T. Layloff, In: Calibration and Validation of Analytical Methods – A Sampling of Current Approaches, Ed. M. Stauffer, 1st ed., Intech Open, London, United Kingdom, 143–163 (2018), https://doi.org/10.5772/intechopen.71765.S. M. Moosavi, S. Ghassabian, In: Calibration and Validation of Analytical Methods – A Sampling of Current Approaches, Ed. M. Stauffer, 1st ed., Intech Open, London, United Kingdom, 109–127 (2018), https://doi.org/10.5772/intechopen.72932.S. M. Moosavi, S. Ghassabian, In: Calibration and Validation of Analytical Methods – A Sampling of Current Approaches, Ed. M. Stauffer, 1st ed., Intech Open, London, United Kingdom, 109–127 (2018), https://doi.org/10.5772/intechopen.72932.M. Thompson, S. L. R. Ellison, R. Wood, Pure Appl. Chem., 74, 835–855 (2002), https://doi.org/10.1351/pac200274050835.M. Thompson, S. L. R. Ellison, R. Wood, Pure Appl. Chem., 74, 835–855 (2002), https://doi.org/10.1351/pac200274050835.T. Nageswara Rao, In: Calibration and Validation of Analytical Methods – A Sampling of Current Approaches, Ed. M. Stauffer, 1st ed., Intech Open, London, United Kingdom, 131–142 (2018), https://doi.org/10.1021/ac00257a001.T. Nageswara Rao, In: Calibration and Validation of Analytical Methods – A Sampling of Current Approaches, Ed. M. Stauffer, 1st ed., Intech Open, London, United Kingdom, 131–142 (2018), https://doi.org/10.1021/ac00257a001.D. Rawat, D. Kumar, Nature of Electronic Transitions and Factors Affecting it: Woodward Fieser Rules and Applications of UV Spectroscopy, Delhi (2014), http://epgp.inflibnet.ac.in/epgpdata/uploads/epgp_content/chemistry/12.organic_spectroscopy/02.nature_of_electronic_transitions_and_the_factors_affecting_it/et/5557_et_et.pdf.D. Rawat, D. Kumar, Nature of Electronic Transitions and Factors Affecting it: Woodward Fieser Rules and Applications of UV Spectroscopy, Delhi (2014), http://epgp.inflibnet.ac.in/epgpdata/uploads/epgp_content/chemistry/12.organic_spectroscopy/02.nature_of_ electronic_transitions_and_the_factors_affecting_it/et/5557_et_et.pdf.K. Florey, In: Analytical Profiles of Drug Substances, Eds. A. Ciba Geigy, H. Y. Aboul Enein, A. A. Al-Badr, S. L. Ali, Elsevier Science Inc., 277–324 (1983).K. Florey, In: Analytical Profiles of Drug Substances, Eds. A. Ciba Geigy, H. Y. Aboul Enein, A. A. Al-Badr, S. L. Ali, Elsevier Science Inc., 277–324 (1983).V. das Gupta, J. Pharm. Sci., 67, 299–302 (1978), https://doi.org/10.1002/jps.2600670305.V. das Gupta, J. Pharm. Sci., 67, 299–302 (1978), https://doi.org/10.1002/jps.2600670305.ACD/pK, version 12.01 for Microsoft Windows, Advanced Chemistry Development, Inc. 8 King Street East, Suite 107, Toronto, Ontario M5C 1B5 Canada (2010), https://www.acdlabs.com/products/perceptaplatform/physchem-suite/pka/#product_demo (accessed November 6, 2022).ACD/pK, version 12.01 for Microsoft Windows, Advanced Chemistry Development, Inc. 8 King Street East, Suite 107, Toronto, Ontario M5C 1B5 Canada (2010), https://www.acdlabs.com/products/perceptaplatform/physchem-suite/pka/#product_demo (accessed November 6, 2022).S. Babić, A. J. M. Horvat, D. Mutavdžić Pavlović, M. Kaštelan-Macan, TrAC – Trends in Analytical Chemistry, 26, 1043–1061 (2007), https://doi.org/10.1016/j.trac.2007.09.004.S. Babić, A. J. M. Horvat, D. Mutavdžić Pavlović, M. Kaštelan-Macan, TrAC – Trends in Analytical Chemistry, 26, 1043–1061 (2007), https://doi.org/10.1016/j.trac.2007.09.004.R. A. Holt, P. G. Seybold, Molecules, 27 (2022), https://doi.org/10.3390/molecules27020385.R. A. Holt, P. G. Seybold, Molecules, 27 (2022), https://doi.org/10.3390/molecules27020385.M. Lapins, S. Arvidsson, S. Lampa, A. Berg, W. Schaal, J. Alvarsson, O. Spjuth, J. Cheminform., 10 (2018), https://doi.org/10.1186/s13321-018-0271-1.M. Lapins, S. Arvidsson, S. Lampa, A. Berg, W. Schaal, J. Alvarsson, O. Spjuth, J. Cheminform., 10 (2018), https://doi.org/10.1186/s13321-018-0271-1.O. Toure, C.-G. Dussap, A. Lebert, Oil & Gas Science and Technology, 68, 281–297 (2013), https://doi.org/10.2516/ogst/2012094ï.O. Toure, C.-G. Dussap, A. Lebert, Oil & Gas Science and Technology, 68, 281–297 (2013), https://doi.org/10.2516/ogst/2012094ï.M. Palomar-Pardavé, G. Alarcón-Angeles, M. T. Ramírez-Silva, M. Romero-Romo, A. RojasHernández, S. Corona-Avendaño, J. Inclusion Phenom. Macrocycl. Chem., 69, 91 (2011), https://doi.org/10.1007/s10847-010-9818-0.M. Palomar-Pardavé, G. Alarcón-Angeles, M. T. Ramírez-Silva, M. Romero-Romo, A. RojasHernández, S. Corona-Avendaño, J. Inclusion Phenom. Macrocycl. Chem., 69, 91 (2011), https://doi.org/10.1007/s10847-010-9818-0.A. K. Rivas-Sánchez, D. S. Guzmán-Hernández, M. T. Ramírez-Silva, M. Romero-Romo, M. PalomarPardavé, Dyes and Pigments, 184, 108641 (2021), https://doi.org/10.1016/j.dyepig.2020.108641.A. K. Rivas-Sánchez, D. S. Guzmán-Hernández, M. T. Ramírez-Silva, M. Romero-Romo, M. PalomarPardavé, Dyes and Pigments, 184, 108641 (2021), https://doi.org/10.1016/j.dyepig.2020.108641.I. E. Widmer, J. J. Puder, C. König, H. Pargger, H. R. Zerkowski, J. Girard, B. Müller, J. Clin. Endocrinology and Metabolism, 90, 4579–4586 (2005), https://doi.org/10.1210/jc.2005-0354.I. E. Widmer, J. J. Puder, C. König, H. Pargger, H. R. Zerkowski, J. Girard, B. Müller, J. Clin. Endocrinology and Metabolism, 90, 4579–4586 (2005), https://doi.org/10.1210/jc.2005-0354.

The authors declare that there are no conflicts of interest present.