zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1548Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESСубстраты для спектроскопии гигантского комбинационного рассеяния на основе покрытого наночастицами серебра пористого кремния для обнаружения низкой концентрации белка денге NS1Nano Silver-Coated Porous Silicon-Based Surface-Enhanced Raman Spectroscopy Substrate for Low Concentration Dengue NS1 Protein DetectionIsmailN. F.IsmailN. F

Пенанг

Pulau Pinang

LeeK. Y.LeeK. Y.

Селангор

Selangor

   

HadisN. S. M.HadisN. S. M.

Пенанг

Pulau Pinang

IsmailL. N.IsmailL. N.

Пенанг

Селангор

Pulau Pinang

Selangor

RahimA. F. A.RahimA. F. A.

Пенанг

Pulau Pinang

AbdullahM. H.AbdullahM. H.

Пенанг

Pulau Pinang

RadzolA. R. M.RadzolA. R. M.

Пенанг

Селангор

Selangor

   

afafrozan944@uitm.edu.myЦентр электротехнических исследований Технологического университета MARACentre for Electrical Engineering Studies, Universiti Teknologi MARA, Cawangan Pulau PinangШкола электротехники, Инженерный колледж, Технологический университет MARACenter of System Studies, School of Electrical Engineering, College of Engineering, Universiti Teknologi MARAШкола электротехники, Инженерный колледж, Технологический университет MARACentre for Electrical Engineering Studies, Universiti Teknologi MARA, Cawangan Pulau Pinang; Center of System Studies, School of Electrical Engineering, College of Engineering, Universiti Teknologi MARAЦентр электротехнических исследований Технологического университета MARA; Малайзийский научно-исследовательский институт старения, Университет ПутраCentre for Electrical Engineering Studies, Universiti Teknologi MARA, Cawangan Pulau Pinang; Malaysian Research Institute on Ageing, Universiti Putra Malaysia202429042024912321321Copyright © Ismail N., Lee K., Hadis N., Ismail L., Rahim A., Abdullah M., Radzol A., 20242024Ismail N., Lee K., Hadis N., Ismail L., Rahim A., Abdullah M., Radzol A.Ismail N., Lee K., Hadis N., Ismail L., Rahim A., Abdullah M., Radzol A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1548

Обнаружение неструктурного белка 1 (NS1) в слюне является потенциальным решением для неинвазивного раннего выявления белка денге NS1. Обнаруженный с помощью иммуноферментного анализа NS1 имеет чувствительность 64.7% и не определяется с помощью наборов экспресс-тестов даже в острых случаях. С использованием спектроскопии гигантского комбинационного рассеяния света (SERS) и кремния в качестве недорогого и распространенного материала разработана пористая кремниевая SERS-подложка с покрытием из наночастиц (НЧ) серебра для обнаружения низкой концентрации NS1. При изготовлении шаблона пористого кремния (PSi) использован электрохимический метод “мокрой лаборатории”, тогда как для осаждения НЧ Ag на PSi — метод капельного осаждения. При использовании родамина в качестве КР-маркера получен коэффициент усиления 53 с пределом обнаружения (LOD) 0.01 мг/мл. Поверхность SERS-субстрата функционализируется антителом к лихорадке денге, что приводит к появлению пиков, связанных с NS1, вплоть до 0.001 мг/мл. Методы литографии позволяют снизить уровень детализации и улучшить характеристики SERS-подложки на основе PSi. 

Detection of nonstructural protein 1 (NS1) in saliva is a potential solution to non-invasive, early dengue detection. However, NS1 in saliva detected using enzyme-linked immunosorbent assay reports only 64.7% sensitivity and is undetectable using rapid test kits even in acute cases. Exploiting surface-enhanced Raman spectroscopy (SERS) and silicon (Si) as a low-cost, abundant material, a nano silver-coated porous silicon SERS substrate was developed for the novel detection of low-concentration NS1. The conventional wet lab electrochemical method was used to fabricate the PSi template, whereas the drop deposition method was used to deposit the AgNP on the PSi. Using rhodamine as the Raman marker, an enhancement factor of 53 was obtained, with a 0.01-mg/mL limit of detection (LOD), which is not spectacularly impressive. However, surprisingly, the SERS substrate surface functionalized with the dengue antibody resulted in the visibility of several peaks related to NS1 up to 0.001 mg/mL. Advanced lithography methods can further lower the LOD and enhance the performance of the PSi-based SERS substrate. Moreover, PSi-based SERS substrate fabrication allows for mass production and low costs. The study successfully developed the SERS substrate for its intended novel application: to detect low-concentration NS1.

пористый кремнийспектроскопия гигантского комбинационного рассеяния светаденгенеинвазивное обнаружениеporous siliconsurface-enhanced Raman spectroscopydenguenon-invasive detectionThis work was supported by the Ministry of Higher Education Malaysia (MOHE) through the Fundamental Research Grant Scheme (FRGS/1/2019/TK05/UITM/02/11).ReferencesWorld Health Organization, Dengue Guidelines for Diagnosis, Treatment, Prevention and Control: New edition, World Health Organization, WHO/HTM/NTD/DEN/2009.1 (2009).World Health Organization, Dengue Guidelines for Diagnosis, Treatment, Prevention and Control: New edition, World Health Organization, WHO/HTM/NTD/DEN/2009.1 (2009).K. L. Anders, N. M. Nguyet, N. T. H. Quyen, T. V. Ngoc, T. V. Tram, T. T. Gan, et al., Am. J. Trop. Medicine and Hygiene, 87, No. 1, 165–170 (2012).K. L. Anders, N. M. Nguyet, N. T. H. Quyen, T. V. Ngoc, T. V. Tram, T. T. Gan, et al., Am. J. Trop. Medicine and Hygiene, 87, No. 1, 165–170 (2012).Machado, Maria Barbério, Gabriel Rios, Danyelle Oliveira, T. M. Silva, S. M. Buzalaf, Marília Costa, S. V. Silva, M. S. Arago, V. D. Siqueira, Walter, Detection of Dengue Virus Infection NS1 Antigen Using Human Saliva (2012).Machado, Maria Barbério, Gabriel Rios, Danyelle Oliveira, T. M. Silva, S. M. Buzalaf, Marília Costa, S. V. Silva, M. S. Arago, V. D. Siqueira, Walter, Detection of Dengue Virus Infection NS1 Antigen Using Human Saliva (2012).C. V. Raman, Nature, 121, No. 3051, 619 (1928), https://doi.org/10.1038/121619b0.C. V. Raman, Nature, 121, No. 3051, 619 (1928), https://doi.org/10.1038/121619b0.X. Guo, D. Wang, R. Khan, Mater. Chem. Phys., art. 123291, 252 (2020), doi: 10.1016/j.matchemphys.2020.123291.X. Guo, D. Wang, R. Khan, Mater. Chem. Phys., art. 123291, 252 (2020), doi: 10.1016/j.matchemphys.2020.123291.D. Kim, J. Kim, J. Henzie, Y. Ko, H. Lim, G. Kwon, J. Na, H.-J. Kim, Y. Yamauchi, J. You, Chem. Eng. J., 419, art. 129445 (2021), doi: 10.1016/j.cej.2021.129445.D. Kim, J. Kim, J. Henzie, Y. Ko, H. Lim, G. Kwon, J. Na, H.-J. Kim, Y. Yamauchi, J. You, Chem. Eng. J., 419, art. 129445 (2021), doi: 10.1016/j.cej.2021.129445.Y. Ma, J. Ma, Y. Zhang, Z. Zhao, C. Gu, D. Chen, J. Zhou, T. Jiang, J. Alloys and Compd., 918, art. 165706 (2022), doi: 10.1016/j.jallcom.2022.165706. 321-9Y. Ma, J. Ma, Y. Zhang, Z. Zhao, C. Gu, D. Chen, J. Zhou, T. Jiang, J. Alloys and Compd., 918, art. 165706 (2022), doi: 10.1016/j.jallcom.2022.165706. 321-9Y. Li, C. Lin, Y. Peng, J. He, Y. Yang, Sens. Actuat. B: Chem., 365, art. 131974 (2022), doi: 10.1016/j.snb.2022.131974.Y. Li, C. Lin, Y. Peng, J. He, Y. Yang, Sens. Actuat. B: Chem., 365, art. 131974 (2022), doi: 10.1016/j.snb.2022.131974.G. Fan, X. Li, S. Xu, C. Dai, Q. Xue, H. Wang, Talanta, 235, art. 122814 (2021), doi: 10.1016/j.talanta.2021.122814.G. Fan, X. Li, S. Xu, C. Dai, Q. Xue, H. Wang, Talanta, 235, art. 122814 (2021), doi: 10.1016/j.talanta.2021.122814.C. Qiu, Z. Cheng, C. Lv, R. Wang, F. Yu, Chin. Chem. Lett., 32, No. 8, 2369–2379 (2021).C. Qiu, Z. Cheng, C. Lv, R. Wang, F. Yu, Chin. Chem. Lett., 32, No. 8, 2369–2379 (2021).M. Moskovits, J. Chem. Phys., 69, No. 9, 4159–4161 (1978), doi: 10.1063/1.437095.M. Moskovits, J. Chem. Phys., 69, No. 9, 4159–4161 (1978), doi: 10.1063/1.437095.P. A. Mosier-Boss, Nanomaterials, 7, No. 6, art. 142 (2017), doi: 10.3390/nano7060142.P. A. Mosier-Boss, Nanomaterials, 7, No. 6, art. 142 (2017), doi: 10.3390/nano7060142.J. Krajczewski, R. Ambroziak, A. Kudelski, Nanomaterials, 11, No. 1, art. 75, 1–25 (2021), doi: 10.3390/nano1101007.J. Krajczewski, R. Ambroziak, A. Kudelski, Nanomaterials, 11, No. 1, art. 75, 1–25 (2021), doi: 10.3390/nano1101007.L. Yang, M. Gong, X. Jiang, D. Yin, X. Qin, B. Zhao, W. Ruan, J. Raman Spectrosc., 46, No. 3, 287–292 (2015), doi: 10.1002/jrs.4645.L. Yang, M. Gong, X. Jiang, D. Yin, X. Qin, B. Zhao, W. Ruan, J. Raman Spectrosc., 46, No. 3, 287–292 (2015), doi: 10.1002/jrs.4645.T. L. Williamson, X. Guo, A. Zukoski, A. Sood, D. J. Díaz, P. W. Bohn, J. Phys. Chem. B, 109, No. 43, 20186–20191 (2005), doi: 10.1021/jp0534939.T. L. Williamson, X. Guo, A. Zukoski, A. Sood, D. J. Díaz, P. W. Bohn, J. Phys. Chem. B, 109, No. 43, 20186–20191 (2005), doi: 10.1021/jp0534939.W. Song, X. Han, L. Chen, Y. Yang, B. Tang, W. Ji, W. Ruan, W. Xu, B. Zhao, Y. Ozaki, J. Raman Spectrosc., 41, No. 9, 907–913 (2010), doi: 10.1002/jrs.2539.W. Song, X. Han, L. Chen, Y. Yang, B. Tang, W. Ji, W. Ruan, W. Xu, B. Zhao, Y. Ozaki, J. Raman Spectrosc., 41, No. 9, 907–913 (2010), doi: 10.1002/jrs.2539.A. G. Cullis, L. T. Canham, P. D. J. Calcott, J. Appl. Phys., 82, No. 3, 909–965 (1997), doi: 10.1063/1.366536.A. G. Cullis, L. T. Canham, P. D. J. Calcott, J. Appl. Phys., 82, No. 3, 909–965 (1997), doi: 10.1063/1.366536.S. P. Low, N. H. Voelcker, Handbook of Porous Silicon, Second ed., 1-2, 533–545 (2018), doi: 10.1007/978-3-319-71381-6_38.S. P. Low, N. H. Voelcker, Handbook of Porous Silicon, Second ed., 1-2, 533–545 (2018), doi: 10.1007/978-3-319-71381-6_38.Q. Shabir, Handbook of Porous Silicon, 395–401 (2014), doi: 10.1007/978-3-319-05744-6_39.Q. Shabir, Handbook of Porous Silicon, 395–401 (2014), doi: 10.1007/978-3-319-05744-6_39.X. Yue, X. Zheng, G. Lv, J. Mo, X. Yu, J. Liu, Z. Jia, X. Lv, J. Tang, Optik, 192, art. 162959 (2019), doi: 10.1016/j.ijleo.2019.162959.X. Yue, X. Zheng, G. Lv, J. Mo, X. Yu, J. Liu, Z. Jia, X. Lv, J. Tang, Optik, 192, art. 162959 (2019), doi: 10.1016/j.ijleo.2019.162959.N. R. Nirala, J. Asiku, H. Dvir, G. Shtenberg, Talanta, 239, art. 123087 (2022), doi: 10.1016/j.talanta.2021.123087.N. R. Nirala, J. Asiku, H. Dvir, G. Shtenberg, Talanta, 239, art. 123087 (2022), doi: 10.1016/j.talanta.2021.123087.V.-T. Vo, V.-D. Phung, S.-W. Lee, Surfaces and Interfaces, 25, art. 101181 (2021), doi: 10.1016/j.surfin.2021.101181.V.-T. Vo, V.-D. Phung, S.-W. Lee, Surfaces and Interfaces, 25, art. 101181 (2021), doi: 10.1016/j.surfin.2021.101181.K. Girel, E. Yantcevich, G. Arzumanyan, N. Doroshkevich, H. Bandarenka, Phys. Status Solidi (a), 213, No. 11, 2911–2915 (2016).K. Girel, E. Yantcevich, G. Arzumanyan, N. Doroshkevich, H. Bandarenka, Phys. Status Solidi (a), 213, No. 11, 2911–2915 (2016).D. Muthukumar, G. Shtenberg, Talanta, 254, 124132 (2023).D. Muthukumar, G. Shtenberg, Talanta, 254, 124132 (2023).N. F. Ismail, A. R. M. Radzol, A. Z. Zulhanip, L. N. Ismail, N. S. Mohamad Hadis, K. Y. Lee, Proceedings – 2020 IEEE EMBS Conference on Biomedical Engineering and Sciences, IECBES 2020, art. 9398818, 147–151 (2021), doi: 10.1109/IECBES48179.2021.9398818.N. F. Ismail, A. R. M. Radzol, A. Z. Zulhanip, L. N. Ismail, N. S. Mohamad Hadis, K. Y. Lee, Proceedings – 2020 IEEE EMBS Conference on Biomedical Engineering and Sciences, IECBES 2020, art. 9398818, 147–151 (2021), doi: 10.1109/IECBES48179.2021.9398818.A. Zaher, P. Hafliger, F. Puppo, G. De Micheli, S. Carrara, IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 – Proceedings, art. 6981759, 448–451 (2014), doi: 10.1109/BioCAS.2014.6981759.A. Zaher, P. Hafliger, F. Puppo, G. De Micheli, S. Carrara, IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 – Proceedings, art. 6981759, 448–451 (2014), doi: 10.1109/BioCAS.2014.6981759.S. Carrara, D. Sacchetto, M.-A. Doucey, C. Baj-Rossi, G. De Micheli, Y. Leblebici, Sens. and Actuat. B: Chem., 171-172, 449–457 (2012), doi: 10.1016/j.snb.2012.04.089.S. Carrara, D. Sacchetto, M.-A. Doucey, C. Baj-Rossi, G. De Micheli, Y. Leblebici, Sens. and Actuat. B: Chem., 171-172, 449–457 (2012), doi: 10.1016/j.snb.2012.04.089.N. F. Ismail, K. Y. Lee, L. N. Ismail, A. A. Rahim, N. M. Hadis, A. R. M. Radzol, In 2022 IEEE-EMBS Conference on Biomedical Engineering and Sciences (IECBES), IEEE, 78–83, doi: 10.1109/IECBES54088.2022.10079329.N. F. Ismail, K. Y. Lee, L. N. Ismail, A. A. Rahim, N. M. Hadis, A. R. M. Radzol, In 2022 IEEE-EMBS Conference on Biomedical Engineering and Sciences (IECBES), IEEE, 78–83, doi: 10.1109/IECBES54088.2022.10079329.R. S. Dariani, Z. Ahmadi, Optik, 124, No. 22, 5353–5356 (2013).R. S. Dariani, Z. Ahmadi, Optik, 124, No. 22, 5353–5356 (2013).R. B. Andreev, Ya. S. Bobovich, A. V. Bortkevich, V. D. Volosov, M. Ya. Tsenter, J. Appl. Spectrosc., 25, No. 2, 1013–1015 (1976), doi: 10.1007/BF00624296.R. B. Andreev, Ya. S. Bobovich, A. V. Bortkevich, V. D. Volosov, M. Ya. Tsenter, J. Appl. Spectrosc., 25, No. 2, 1013–1015 (1976), doi: 10.1007/BF00624296.X. N. He, Y. Gao, M. Mahjouri-Samani, P. N. Black, J. Allen, M. Mitchell, W. Xiong, Y. S. Zhou, L. Jiang, Y. F. Lu, Nanotechnology, 23, No. 20, art. 205702 (2012), doi: 10.1088/0957-4484/23/20/205702.X. N. He, Y. Gao, M. Mahjouri-Samani, P. N. Black, J. Allen, M. Mitchell, W. Xiong, Y. S. Zhou, L. Jiang, Y. F. Lu, Nanotechnology, 23, No. 20, art. 205702 (2012), doi: 10.1088/0957-4484/23/20/205702.L. Liu, S. Hou, X. Zhao, C. Liu, Z. Li, C. Li, S. Xu, G. Wang, J. Yu, C. Zhang, B. Man, Nanomaterials, 10, No. 12, art. 2371, 1–16 (2020), doi: 10.3390/nano10122371.L. Liu, S. Hou, X. Zhao, C. Liu, Z. Li, C. Li, S. Xu, G. Wang, J. Yu, C. Zhang, B. Man, Nanomaterials, 10, No. 12, art. 2371, 1–16 (2020), doi: 10.3390/nano10122371.P. Hildebrandt, M. Stockhurger, J. Phys. Chem., 88, No. 24, 5935–5944 (1984), doi: 10.1021/j150668a038.P. Hildebrandt, M. Stockhurger, J. Phys. Chem., 88, No. 24, 5935–5944 (1984), doi: 10.1021/j150668a038.Z. Movasaghi, S. Rehman, I. U. Rehman, Appl. Spectrosc. Rev., 42, No. 5, 493–541 (2007), doi: 10.1080/05704920701551530.Z. Movasaghi, S. Rehman, I. U. Rehman, Appl. Spectrosc. Rev., 42, No. 5, 493–541 (2007), doi: 10.1080/05704920701551530.R. P. Kengne-Momo, P. Daniel, F. Lagarde, Y. L. Jeyachandran, J. F. Pilard, M. J. Durand-Thouand, G. Thouand, Int. J. Spectrosc., 2012 (2012).R. P. Kengne-Momo, P. Daniel, F. Lagarde, Y. L. Jeyachandran, J. F. Pilard, M. J. Durand-Thouand, G. Thouand, Int. J. Spectrosc., 2012 (2012).A. Rygula, K. Majzner, K. M. Marzec, A. Kaczor, M. Pilarczyk, M. Baranska, J. Raman Spectrosc., 44, No. 8, 1061–1076 (2013).A. Rygula, K. Majzner, K. M. Marzec, A. Kaczor, M. Pilarczyk, M. Baranska, J. Raman Spectrosc., 44, No. 8, 1061–1076 (2013).F. Adar, Spectroscopy, 37, No. 2, 9–12, 25 (2022).F. Adar, Spectroscopy, 37, No. 2, 9–12, 25 (2022).

The authors declare that there are no conflicts of interest present.