zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1720Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESОценка константы стабильности витамина В1 по стехиометрическим зависимостям методом видимой спектроскопииEvaluation of stability constant for vit В1 depending on stoichiometric curves by visible spectroscopyHamodyA. S.HamodyAzhar S.

Багдад

Baghdad

AlassafN. A.AlassafNahla A.

Багдад

Baghdad

nahla.alassaf@gmail.comSalehR. A.SalehResala A.

Багдад

Baghdad

ZankanahF. H.ZankanahFaeza H.

Багдад

Baghdad

DikranS. B.DikranSarmad B.

Багдад

Baghdad

Педагогический колледж Ибн Аль-Хайтама, Адхамия, Багдадский университетDepartment of Chemistry, College of Education for Pure Science Ibn Al-Haitham, Adhamiya, University of Baghdad202418112024916914914Copyright © Hamody A.S., Alassaf N.A., Saleh R.A., Zankanah F.H., Dikran S., 20242024Hamody A., Alassaf N., Saleh R., Zankanah F., Dikran S.Hamody A.S., Alassaf N.A., Saleh R.A., Zankanah F.H., Dikran S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1720

Предложен спектрофотометрический метод для измерения содержания витамина B1 в чистых и фармацевтических составах. Сульфацетамид (SFA) диазотируется, затем соединяется с витамином B1 в щелочной среде для получения окрашенного комплекса азокрасителя с константой устойчивости 5.597×105 л/моль. Полученный продукт стабилен, имеет максимальную длину волны поглощения 489.5 нм, молярную поглощательную способность 10108 л/моль×см, чувствительность Санделла 0.0334 мкг/см2, предел обнаружения 0.0135 мкг/мл, закон Бера соблюдается в диапазоне концентраций 0.2–20.0 мкг/мл. Константа устойчивости и стехиометрия полученного азокрасителя рассчитаны с использованием методов непрерывного изменения (Джоба) и молярного отношения. Предложенный подход успешно использован при определении витамина В1 в его фармацевтических составах.

The measurement of vitamin B1 in pure and pharmaceutical formulations was proposed by using a straightforward and sensitive spectrophotometric approach. Sulfacetamide (SFA) is diazotized, then coupled with vitamin B1 in alkaline media to produce a coloured azo dye complex with a stability constant of 5.597×105 L/mol. The product is stable, with a maximum absorption wavelength of 489.5 nm, molar absorptivity of 10108 L/mol ∙ cm, Sandell’s sensitivity of 0.0334 μg/cm2, detection limit of 0.0135 μg/mL, and Beer's law being observed over the concentration range of 0.2–20.0 μg/mL. The stability constant and stoichiometry of the produced azo dye were calculated using the continuous variation (Job’s) and mole ratio methods. The suggested approach was used successfully in the determination of vitamin B1 in its pharmaceutical formulations.

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The authors declare that there are no conflicts of interest present.