zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1721Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESСравнение сигналов по ширине линии hod в спектрах ЯМР 400 МГц образцов крови и мочи здоровых и больных пациентовSeparation of line widths of hod peaks of healthy and diseased blood and urine groups using 400 MHz NMRSakarM. N.SakarMehmet Nafi

Диярбакыр, Стамбул

Diyarbakir, İstanbul

YilmazU. N.YilmazUtku Nezih

Диярбакыр

Diyarbakir

utkunezih@gmail.comKöylüM. Z.KöylüMehmet Zafer

Диярбакыр

Diyarbakir

Мемориальная больница Диярбакыра; Медицинская школа Университета УскюдарDepartment of Obstetrics and Gynecology, Memorial Diyarbakir Hospital; Uskudar University Medical SchoolУниверситет ДиджлеDepartment of Oral-Maxillofacial Surgery, Faculty of Dentistry, Dicle UniversityФакультет естественных наук Университета ДиджлеDepartment of Physics, Faculty of Science, Dicle University202418112024916915915Copyright © Sakar M.N., Yilmaz U.N., Köylü M., 20242024Sakar M.N., Yilmaz U.N., Köylü M.Sakar M., Yilmaz U., Köylü M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1721

Проведено сравнение ширины линии ядерного магнитного резонанса (ЯМР) HOD образцов крови здоровых и больных пациентов, крови челюстных кист, а также образцов мочи здоровых и больных пациентов. Собраны кровь, сыворотка и моча 29 онкологических больных, 17 пациентов с диабетом, 28 здоровых добровольцев и 20 образцов крови из челюстных кист. Смеси приготовлены путем добавления образца объемом 0.02 мл к 0.98 мл D2O. Измерения одноимпульсного протонного ЯМР проведены на частоте 400 МГц, ширина линии получена из половины высоты пика HOD крови. Статистические оценки показывают, что ширина линии в случае “онкологической” крови отличается от нормальной крови (р = 0.032), в случае “диабетической” крови не отличается (p = 0.072), тогда как для других групп и кровавых кист челюсти наблюдаются отличия от нормальной крови (p < 0.05). Аналогичные результаты получены для мочи. Ширина линий показывает умеренную корреляцию  с каждой из групп альбумина и общего белка (R ≈ 0.56). Сравнение жидкостей здоровых и больных пациентов может быть сделано путем измерения ширины линии HOD в высоком поле ЯМР.  Результаты позволяют предположить, что релаксивности альбумина (Ser-Alb) и общего белка  (Ser-TP) являются потенциальными диагностическими индикаторами “онкологической” крови.  

Line width studies of high field nuclear magnetic resonance (NMR) peaks have been seen more frequently in the recent literature. This study aims to compare the NMR line widths of HOD peaks of healthy and diseased blood, and densely bloody cysts taken from the jaw. Comparisons of HOD peak line widths  in healthy and diseased urine were also made. For this purpose, the blood, serum, and urine of 29 cancer patients, 17 diabetic patients, 28 healthy volunteers, and 20 intensely bloody samples from jaw cysts were collected. Mixtures were prepared by adding the 0.02-mL sample to 0.98 mL D2O. Single pulse proton NMR measurements were performed at 400 MHz, and line widths were obtained from the half-height of the blood HOD peak. Statistical evaluations show that the line width of cancerous blood is different from normal blood (P = 0.032), while the line width of diabetic blood is not different (p = 0.072), whereas other groups and bloody jaw cysts are completely different (p<0.05). Similar results were found for urine groups. Line widths show a moderate correlation with each of the albumin and total protein groups (R around 0,56). Present data suggest that a comparison of healthy and diseased body fluids can be made by line width measurements of the HOD peak in the high NMR field. Data also suggest that the relaxivities of albumin (SerAlb), total protein (Ser-TP) are potential diagnostic indicators for cancerous blood.

ЯМР 400 МГцсмеси с избыточным D2Oширина линиикровьмочакиста400 MHz NMRmixtures with excessive D2Oline widthsbloodurinebloody cystsWe would like to thank Mehmet Ali Kaplan from the Oncology Clinic and Abbas Zulfuogullari from Internal Medicine for their assistance in collecting blood and urine samples. We would also like to thank Erkan Yilmaz for his assistance in measuring hematological and biochemical parameters.ReferencesA. Zipp, T. L. James, I. D. Kuntz, S. B. Shohet, Biochim. Biophys. Acta, 473, 29l–303 (1976).A. Zipp, T. L. James, I. D. Kuntz, S. B. Shohet, Biochim. Biophys. Acta, 473, 29l–303 (1976).P. Liu, L. Chalak, L. C. Krishnamurthy, I. Mir, S.-L. Peng, H. Huang, et al., Magn. Res. Med., 275, No. 4, 1730–1735 (2016).P. Liu, L. Chalak, L. C. Krishnamurthy, I. Mir, S.-L. Peng, H. Huang, et al., Magn. Res. Med., 275, No. 4, 1730–1735 (2016).D. P. Cistola, M. D. Robinson, TrAC. Trends Anal. Chem. Part A, 83, 53–64 (2016).D. P. Cistola, M. D. Robinson, TrAC. Trends Anal. Chem. Part A, 83, 53–64 (2016).M. J. Kransdorf, J. S. Jelinek, R. P. Moser, J. A. Utz, A. C. Brower, T. M. Hudson, et al., AJR Am. J. Roentgenol., 153, 541–547 (1989)M. J. Kransdorf, J. S. Jelinek, R. P. Moser, J. A. Utz, A. C. Brower, T. M. Hudson, et al., AJR Am. J. Roentgenol., 153, 541–547 (1989)U. N. Yilmaz, B. D. Yilmaz, M. Z. Köylü, J. Appl. Spectrosc., 89, No. 1, 69–74 (2022).U. N. Yilmaz, B. D. Yilmaz, M. Z. Köylü, J. Appl. Spectrosc., 89, No. 1, 69–74 (2022).A. Carrington, A. D. McLachlan. Introduction to Magnetic Resonance, Harper & Row, New York (1967).A. Carrington, A. D. McLachlan. Introduction to Magnetic Resonance, Harper & Row, New York (1967).G. Mladenov, V. S. Dimitrov, Magn. Res. Chem., 39, 672–680 (2001).G. Mladenov, V. S. Dimitrov, Magn. Res. Chem., 39, 672–680 (2001).K. Chen, N. Tjandra, J. Magn. Res., 197, 71–76 (2009).K. Chen, N. Tjandra, J. Magn. Res., 197, 71–76 (2009).M. J. Wilhelm, H. H. Ong, F. W. Wehrli, Proc. 20th Annual Meeting of ISMRM, Melbourne, Victoria, Australia, 2394 (2012).M. J. Wilhelm, H. H. Ong, F. W. Wehrli, Proc. 20th Annual Meeting of ISMRM, Melbourne, Victoria, Australia, 2394 (2012).A. Ahlner, M. Carlsson, B. H. Jonsson, P. Lundström, J. Biomol. NMR, 56, 191(2013).A. Ahlner, M. Carlsson, B. H. Jonsson, P. Lundström, J. Biomol. NMR, 56, 191(2013).M. Niklasson, R. Otten, A. Ahlner, C. Andresen, J. Schlagnitweit, K. Petzold, P. Lundström, J. Biomol. NMR, 69, No. 2, 93–99 (2017).M. Niklasson, R. Otten, A. Ahlner, C. Andresen, J. Schlagnitweit, K. Petzold, P. Lundström, J. Biomol. NMR, 69, No. 2, 93–99 (2017).A. B. Siemer, K. Y. Huang, A. E. McDermott, PLoS One, 7, e47242 (2012), doi: 10.1371/journal.pone.0047242.A. B. Siemer, K. Y. Huang, A. E. McDermott, PLoS One, 7, e47242 (2012), doi: 10.1371/journal.pone.0047242.S. Maity, R. K. Gundampati, T. K. S. Kumar, Nat. Prod. Commun., 14, No. 5, 1–17 (2019), https://doi.org/10.1177/1934578X19849296.S. Maity, R. K. Gundampati, T. K. S. Kumar, Nat. Prod. Commun., 14, No. 5, 1–17 (2019), https://doi.org/10.1177/1934578X19849296.A. A. Malär, S. S. Penzel, G. M. Camenisch, T. Wiegand, A. Samoson, A. Böckmann, et al., Phys. Chem. Chem. Phys., 21, 18850–18865 (2019).A. A. Malär, S. S. Penzel, G. M. Camenisch, T. Wiegand, A. Samoson, A. Böckmann, et al., Phys. Chem. Chem. Phys., 21, 18850–18865 (2019).S. Y. Hauge, M. Esmaeili, T. E. Sjøbakk, R. Grüner, K. Woiek, H. M. Werner, et al., Acta Radiol., 59, No. 4, 497–505 (2018).S. Y. Hauge, M. Esmaeili, T. E. Sjøbakk, R. Grüner, K. Woiek, H. M. Werner, et al., Acta Radiol., 59, No. 4, 497–505 (2018).V. Kumar, D. K. Dwivedi, N. R. Jagannathan, NMR Biomed., 27, No. 1, 80–89 (2014).V. Kumar, D. K. Dwivedi, N. R. Jagannathan, NMR Biomed., 27, No. 1, 80–89 (2014).S. Mendes, C. A. Rinne, J. C. Schmidt, D. D. Berndt, C. Walter, Clin. Oral. Inv., 24, No. 2, 547–557 (2020).S. Mendes, C. A. Rinne, J. C. Schmidt, D. D. Berndt, C. Walter, Clin. Oral. Inv., 24, No. 2, 547–557 (2020).K. Zia, T. Siddiqui, S. Ali, I. Farooq, M. S. Zafar, Z. Khurshid. Eur. J. Dent., 13, No. 1, 124–128 (2019).K. Zia, T. Siddiqui, S. Ali, I. Farooq, M. S. Zafar, Z. Khurshid. Eur. J. Dent., 13, No. 1, 124–128 (2019).H. M. De Feyter, K. L. Behar, Z. A. Corbin, R. K. Fulbright, P. B. Brown, S. McIntyre S, et al., Sci. Adv., 4, eaat7314 (2018), doi: 10.1126/sciadv.Aat 7314.H. M. De Feyter, K. L. Behar, Z. A. Corbin, R. K. Fulbright, P. B. Brown, S. McIntyre S, et al., Sci. Adv., 4, eaat7314 (2018), doi: 10.1126/sciadv.Aat 7314.F. N. Wang, S. L. Peng, C. T. Lu, H. H. Peng, T. C. Yeh, NMR Biomed., 26, No. 6, 692–698 (2013).F. N. Wang, S. L. Peng, C. T. Lu, H. H. Peng, T. C. Yeh, NMR Biomed., 26, No. 6, 692–698 (2013).A. Yilmaz, B. Zengin, F. S. Ulak, J. Appl. Spectrosc., 81, No. 3, 365–370 (2014).A. Yilmaz, B. Zengin, F. S. Ulak, J. Appl. Spectrosc., 81, No. 3, 365–370 (2014).A. Yilmaz, B. Zengin, J. Appl. Spectrosc., 80, No. 3, 335–340 (2013).A. Yilmaz, B. Zengin, J. Appl. Spectrosc., 80, No. 3, 335–340 (2013).U. N. Yılmaz, B. D. Yılmaz, J. Appl. Spectrosc., 87, No. 5, 946–950 (2020).U. N. Yılmaz, B. D. Yılmaz, J. Appl. Spectrosc., 87, No. 5, 946–950 (2020).U. N. Yilmaz, B. D. Yilmaz, M. Z. Köylü, J. Appl. Spectrosc., 89, No. 6, 1100–1106 (2023).U. N. Yilmaz, B. D. Yilmaz, M. Z. Köylü, J. Appl. Spectrosc., 89, No. 6, 1100–1106 (2023).U. N. Yilmaz, R. Güner, B. Erol, Biol. Trace Elem. Res., 133, No. 2, 144–152 (2009).U. N. Yilmaz, R. Güner, B. Erol, Biol. Trace Elem. Res., 133, No. 2, 144–152 (2009).A. Yilmaz, O. Denli, I. Demirel, Turk. J. Phys., 13, 219–222 (1989).A. Yilmaz, O. Denli, I. Demirel, Turk. J. Phys., 13, 219–222 (1989).M. E. Fabry, M. Eisenstadt, Biophys. J., 15, 1101–1110 (1975).M. E. Fabry, M. Eisenstadt, Biophys. J., 15, 1101–1110 (1975).B. R. R. Persson, L. Malmgren, L. G. Salford, Acta Sci. Lunden., ISSN 1651-5013, 1–22 (2012).B. R. R. Persson, L. Malmgren, L. G. Salford, Acta Sci. Lunden., ISSN 1651-5013, 1–22 (2012).R. G. Bryan, K. Marill, C. Blackmore, C. Francis, Magn. Res. Med., 13, 133–144 (1990).R. G. Bryan, K. Marill, C. Blackmore, C. Francis, Magn. Res. Med., 13, 133–144 (1990).H. H. Raeymeakers, D. Borgyhs, H. Eisendraht, Magn. Res. Med., 6, 212–216 (1988).H. H. Raeymeakers, D. Borgyhs, H. Eisendraht, Magn. Res. Med., 6, 212–216 (1988).P. A. Bottomley, C. J. Hardy, R. E. Argersinger, A. Allen-Moore, Med. Phys., 14, 127 (1987).P. A. Bottomley, C. J. Hardy, R. E. Argersinger, A. Allen-Moore, Med. Phys., 14, 127 (1987).M. V. D. Graaf, Eur. Biophys. J., 39, No. 4, 527–540 (2010).M. V. D. Graaf, Eur. Biophys. J., 39, No. 4, 527–540 (2010).Y. Li, I. Park, S. J. Nelson, Cancer J., 21, No. 2, 123–128 (2015).Y. Li, I. Park, S. J. Nelson, Cancer J., 21, No. 2, 123–128 (2015).U. N. Yilmaz, F. Yaman, S. S. Atilgan, Dentomaxillofac. Radiol., 41, No. 5, 385–391 (2012).U. N. Yilmaz, F. Yaman, S. S. Atilgan, Dentomaxillofac. Radiol., 41, No. 5, 385–391 (2012).

The authors declare that there are no conflicts of interest present.