zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1775Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESРазработка и валидация колориметрического комплекса с переносом заряда для оценки цефподоксима проксетила в нерасфасованном виде и фармацевтических препаратахDEVELOPMENT AND VALIDATION OF THE COLORIMETRIC CHARGE TRANSFER COMPLEX FOR ESTIMATION OF CEFPODOXIME PROXETIL IN BULK AND PHARMACEUTICAL PREPARATIONSOzdemirE.OzdemirElif

Стамбул

Pharmacy Faculty, Department of Analytical Chemistry 

Istanbul

KarasakalA.KarasakalAyca

Текирдаг

Science and Letters Faculty, Department of Chemistry 

Tekirdag

akarasakal@nku.edu.trСтамбульский университет Йени ЮзылIstanbul Yeni Yuzyil UniversityТекирдагский университет имени Намика КемаляTekirdag Namik Kemal University202507022025921136136Copyright © Ozdemir E., Karasakal A., 20252025Ozdemir E., Karasakal A.Ozdemir E., Karasakal A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1775

Разработан простой, быстрый и чувствительный спектрофотометрический метод определения цефподоксима проксетила – антибиотика группы цефалоспоринов третьего поколения, антибактериального β-лактамного средства, которое используется при лечении острого отита, фарингита, тонзиллита и инфекций нижних дыхательных путей (пневмонии и бронхита), в фармацевтических препаратах. Метод основан на взаимодействиях с переносом заряда цефподоксима проксетила как донора n-электронов с 7,7,8,8-тетрацианохинодиметаном (TCNQ) в качестве π-акцептора. Метод проверен на соответствие требованиям ICH. Поглощение измерено при 596 нм. Метод показал линейность в диапазоне концентраций 3.0–15.0 мкг/мл, коэффициент корреляции 0.9997, LOD = 0.036 мкг/мл и LOQ = 0.108 мкг/мл. Восстановление в диапазоне 99.97–101.3% соответствует методу стандартных добавок. Эффективность предлагаемого метода оценена с помощью t-критерия Стьюдента и F-критерия. Разработанный метод успешно применен для оценки цефподоксима проксетилa в чистом виде и в фармацевтическом препарате. 

Cefpodoxime proxetil (CFP) is a third-generation cephalosporins group antibiotic which is an antibacterial beta-lactam agent that is used in the treatment of acute otitis, pharyngitis, tonsillitis and lower respiratory tract infections (e.g. pinomonia and bronchitis). A simple, fast and sensitive spectrophotometric method was developed for the determination of cefpodoxime proxetile in bulk and pharmaceutical preparations. The proposed method was based on the charge transfer interactions of cefpodoxime proxetil as n-electron donor with 7,7,8,8-tetracyanoquinodimethane (TCNQ) as the π-acceptor. In addition, the described method was validated in accordance with the requirements of ICH guidelines. The absorbance was measured at 596 nm. The parameter of linearity, LOD, LOQ, accuracy, precision and recovery were also evaluated. The assay was linear over the concentration range of 3.0–15.0 µg/mL. The correlation coefficient was obtained 0.9997 for CFP. LOD and LOQ values were found to be 0.036 and 0.108 µg/mL, respectively. Recovery values were obtained ranging from 99.97 to 101.3% according to the standard addition method. The performance of the proposed method was evaluated in terms of the student’s t-test and variance ratio Ftest to find out the significance of the proposed methods over the reference spectrophotometric method. The developed method was successfully applied for estimation of CFP in a pure form and as a pharmaceutical preparate.

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The authors declare that there are no conflicts of interest present.