zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-1936Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESОпределение комбинации антимикробных препаратов методами in silico и УФ-спектроскопии со спектральным разрешениемUV Method for Determination of Novel Combination of Antimicrobials by Spectral Resolution and in silico Pharmacokinetic StudyPrashantK. N.PrashantK. N.

Нагар, Карнатака

K. Naga Prashant - Faculty of Pharmacy, Sri Adichunchanagiri College of Pharmacy.

B.G. Nagar, Karnataka

VenkateshK. G.VenkateshK. G.

Нагар, Карнатака

Faculty of Pharmacy, Sri Adichunchanagiri College of Pharmacy.

B.G. Nagar, Karnataka

SuvarnaY.SuvarnaY.

Нагар, Карнатака

Yenduri Suvarna - Faculty of Pharmacy, Sri Adichunchanagiri College of Pharmacy.

B.G. Nagar, Karnataka

Suvarna.ph52@gmail.comФармацевтический колледж Шри Адичунчанагири Университета АдичунчанагириAdichunchanagiri University202507092025924558558Copyright © Prashant K.N., Venkatesh K.G., Suvarna Y., 20252025Prashant K.N., Venkatesh K.G., Suvarna Y.Prashant K.N., Venkatesh K.G., Suvarna Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/1936

Представлены четыре УФ-спектрофотометрических метода для одновременного определения тетрациклина HCl и тинидазола в бинарных смесях и таблетированных лекарственных формах. Для разрешения спектрального перекрытия использованы методы первой производной, площади под кривой, двойной длины волны и разности отношений. Методы продемонстрировали линейность в диапазоне 10–40 мкг/мл для обоих препаратов, в то время как пределы обнаружения находились в диапазоне 1.562–2.974 мкг/мл для тетрациклина HCl и 0.077–1.65 мкг/мл для тинидазола. Результаты ANOVA не показали статистических различий между методами. Разработанные методы устойчивые и экологически чистые по оценке, проведенной методами MoGAPI, RGB и BAGI. Исследование in silico в отношении ферментов CYP450 не выявило взаимодействия лекарственных средств. Разработанные методы показали хорошее соответствие параметрам валидации и признаны эффективными, экономичными, не содержащими токсичных растворителей.

Tetracycline HCl and Tinidazole combination drugs are used for antimicrobial therapy. In order to quantify them in dosage forms by UV spectroscopy, resolving the spectral overlap is necessary to avoid interference. The present study introduced four simple, sustainable, mathematically processed UV spectrophotometric methods for the simultaneous determination of Tetracycline HCl and Tinidazole in binary mixtures and tablet dosage forms. The approaches used for resolving spectral overlap include first derivative, area under curve, dual wave length and ratio difference methods. The methods demonstrated linearity in the range of 10–40 μg/mL for both drugs, while detection limits were in the range of 1.562–2.974 mcg/mL for tetracycline HCl and tinidazole showed 0.077–1.65 μg/mL. ANOVA results showed no statistical difference among the methods. Sustainability assessment made by MoGAPI, RGB, and BAGI declared the developed method to be sustainable and eco-friendly. In silico drug-drug interaction study with respect to CYP450 enzymes showed no drug interactions. The developed method showed good agreement with validation parameters and was found to be efficient, economical, free of toxic solvents, and the complex sample preparation procedures indicated its sustainability.

УФ-спектроскопияспектральное разрешениематематические манипуляцииустойчивостьисследование in silicoUV spectroscopyspectral resolutionmathematical manipulationssustainabilityin silico studiesAll the authors are grateful to the staff of Adichunchanagiri University for their continuous support.Referenceshttps://www.Chemspider.Com/Chemical-Structure.5279.Html (2024).https://www.Chemspider.Com/Chemical-Structure.5279.Html (2024).C. Zhang, R. Liu, R. Liu, W. Cui, Y. Sun, W.-D. Yang, Ultrason Sonochem., 110, 107056 (2024).C. Zhang, R. Liu, R. Liu, W. Cui, Y. Sun, W.-D. Yang, Ultrason Sonochem., 110, 107056 (2024).A. Rusu, E. L. Buta, Pharmaceutics, 13, 2085 (2021).A. Rusu, E. L. Buta, Pharmaceutics, 13, 2085 (2021).https://www.Chemspider.Com/Chemical-Structure.10257122.Html (2024).https://www.Chemspider.Com/Chemical-Structure.10257122.Html (2024).L. T. Nguyen, V. B. Nguyen, T. V. Tran, H. Q. Duong, L. T. T. Le, M. H. T. Phuong, T. Nguyen, Gastroenterol Insights, 13, 365 (2022).L. T. Nguyen, V. B. Nguyen, T. V. Tran, H. Q. Duong, L. T. T. Le, M. H. T. Phuong, T. Nguyen, Gastroenterol Insights, 13, 365 (2022).Y. Wang, Q. Wang, Z. Zhao, A. Liu, Y. Tian, J. Qin, Talanta, 190, 284 (2018).Y. Wang, Q. Wang, Z. Zhao, A. Liu, Y. Tian, J. Qin, Talanta, 190, 284 (2018).B. Tegegne, B. S. Chandravanshi, F. Zewge, L. Pillay, L. Chimuka, Bull. Chem. Soc. Ethiop., 35, 17 (2021).B. Tegegne, B. S. Chandravanshi, F. Zewge, L. Pillay, L. Chimuka, Bull. Chem. Soc. Ethiop., 35, 17 (2021).M. Gashaw, T. Layloff, A. Hymete, A. Ashenef, BMC Chem., 18, 82 (2024).M. Gashaw, T. Layloff, A. Hymete, A. Ashenef, BMC Chem., 18, 82 (2024).M. N. Uddin, A. Hasnaine, Md. S. Haque, S. Das, M. Uddin, D. Chakraborty, M. Mostafa, J. Food Prot., 87, 100279 (2024).M. N. Uddin, A. Hasnaine, Md. S. Haque, S. Das, M. Uddin, D. Chakraborty, M. Mostafa, J. Food Prot., 87, 100279 (2024).N. A. Alassaf, A. S. Hamody, S. B. Dikran1, F. H. Zankanah, Int. J. Drug Delivery Technology, 9 (2019).N. A. Alassaf, A. S. Hamody, S. B. Dikran1, F. H. Zankanah, Int. J. Drug Delivery Technology, 9 (2019).N. C. Rompicherla, M. G. Ahmed, P. Nayak, Int. J. Pharm. Sci. Rev. Res., 28, 207 (2014).N. C. Rompicherla, M. G. Ahmed, P. Nayak, Int. J. Pharm. Sci. Rev. Res., 28, 207 (2014).T. N. Al-Sabha, M. Y. Dhamra, T. S. Al-Ghabsha, Eur. Chem. Bull., 6, 336 (2017).T. N. Al-Sabha, M. Y. Dhamra, T. S. Al-Ghabsha, Eur. Chem. Bull., 6, 336 (2017).Y.-M. Liang, H. Yang, B. Zhou, Y. Chen, M. Yang, K.-S. Wei, X.-F. Yan, C. Kang, Anal. Chim. Acta, 1191, 339269 (2022).Y.-M. Liang, H. Yang, B. Zhou, Y. Chen, M. Yang, K.-S. Wei, X.-F. Yan, C. Kang, Anal. Chim. Acta, 1191, 339269 (2022).A. Rahaman Shaik, A. S. Rahaman, Int. J. Pharm. Ind. Res., 3, 295 (2013).A. Rahaman Shaik, A. S. Rahaman, Int. J. Pharm. Ind. Res., 3, 295 (2013).S. Gummadi, D. Thota, S. V. Varri, P. Vaddi, V. L. N. S. Rao, Int. Current Pharm. J., 1, 317 (2012).S. Gummadi, D. Thota, S. V. Varri, P. Vaddi, V. L. N. S. Rao, Int. Current Pharm. J., 1, 317 (2012).R. K. Maheshwari, M. S. Rajput, S. Sinha, Asian J. Pharm., 3, 319 (2009).R. K. Maheshwari, M. S. Rajput, S. Sinha, Asian J. Pharm., 3, 319 (2009).A. Goyal, S. Choudhary, G. D. Singh, Int. J. Pharm. Chem. Analysis, 2, 22 (2015).A. Goyal, S. Choudhary, G. D. Singh, Int. J. Pharm. Chem. Analysis, 2, 22 (2015).A. Pyka-Pająk, Processes, 12, 643 (2024).A. Pyka-Pająk, Processes, 12, 643 (2024).D. B. Meshram, S. Desai, Int. J. Adv. Res., 2, 855–863 (2014).D. B. Meshram, S. Desai, Int. J. Adv. Res., 2, 855–863 (2014).C. Zhang, R. Liu, R. Liu, W. Cui, Y. Sun, W.-D. Yang, Ultrason Sonochem., 110, 107056 (2024).C. Zhang, R. Liu, R. Liu, W. Cui, Y. Sun, W.-D. Yang, Ultrason Sonochem., 110, 107056 (2024).M. Tobiszewski, A. Mechlińska, J. Namieśnik, Chem. Soc. Rev., 39, 2869 (2010).M. Tobiszewski, A. Mechlińska, J. Namieśnik, Chem. Soc. Rev., 39, 2869 (2010).N. P. K. Yenduri Suvarna, Recent Res. Trends Pharm. Sci., 2, Integrated Publ. (2023).N. P. K. Yenduri Suvarna, Recent Res. Trends Pharm. Sci., 2, Integrated Publ. (2023).R. A. Sayed, A. R. Mohamed, A. Shalaby, H. Ibrahim, Sust. Chem. Pharm., 32, 100976 (2023).R. A. Sayed, A. R. Mohamed, A. Shalaby, H. Ibrahim, Sust. Chem. Pharm., 32, 100976 (2023).S. Yenduri, K. N. Prashant, Futur. J. Pharm. Sci., 10, 128 (2024).S. Yenduri, K. N. Prashant, Futur. J. Pharm. Sci., 10, 128 (2024).A. V. Dmitriev, A. V. Rudik, D. A. Karasev, P. V. Pogodin, A. A. Lagunin, D. A. Filimonov, V. V. Poroikov, Pharmaceutics, 13, 538 (2021).A. V. Dmitriev, A. V. Rudik, D. A. Karasev, P. V. Pogodin, A. A. Lagunin, D. A. Filimonov, V. V. Poroikov, Pharmaceutics, 13, 538 (2021).S. I. Aboras, A. A. Megahed, F. El-Yazbi, H. M. Maher, BMC Chem., 18, 50 (2024).S. I. Aboras, A. A. Megahed, F. El-Yazbi, H. M. Maher, BMC Chem., 18, 50 (2024).S. A. Mahmoud, A. M. El-Kosasy, F. A. Fouad, J. Chromatogr. Sci., 61, 907 (2023).S. A. Mahmoud, A. M. El-Kosasy, F. A. Fouad, J. Chromatogr. Sci., 61, 907 (2023).K. S. Natraj, Y. Suvarna, G. Prasanti, S. V Saikumar, Int. Res. J. Pharm., 4, 178 (2013).K. S. Natraj, Y. Suvarna, G. Prasanti, S. V Saikumar, Int. Res. J. Pharm., 4, 178 (2013).K. H. Elgendy, M. Zaky, A. E. Mohamed, M. Altorky, S. Fadel, BMC Chem., 18, 115 (2024).K. H. Elgendy, M. Zaky, A. E. Mohamed, M. Altorky, S. Fadel, BMC Chem., 18, 115 (2024).M. K. Deka, A. Ansary, T. K. Das, A. K. Das, B. J. Sahariah, M. Majumder, Green Anal. Chem., 8, 100088 (2024).M. K. Deka, A. Ansary, T. K. Das, A. K. Das, B. J. Sahariah, M. Majumder, Green Anal. Chem., 8, 100088 (2024).I. J. Soni, H. J. Panchal, Indian J. Pharm. Biol. Res., 2, 76 (2014).I. J. Soni, H. J. Panchal, Indian J. Pharm. Biol. Res., 2, 76 (2014).Z. Kayali, R. H. Obaydo, A. Alhaj Sakur, Heliyon, 9, e15260 (2023).Z. Kayali, R. H. Obaydo, A. Alhaj Sakur, Heliyon, 9, e15260 (2023).K. Attala, S. S. Abd El-Hay, M. M. El-Henawee, M. S. Eissa, Spectrochim. Acta A: Mol. Biomolec. Spectrosc., 255, 119715 (2021).K. Attala, S. S. Abd El-Hay, M. M. El-Henawee, M. S. Eissa, Spectrochim. Acta A: Mol. Biomolec. Spectrosc., 255, 119715 (2021).M. G. Fawzy, W. E. Hassan, A. A. Mostafa, R. A. Sayed, Spectrochim. Acta A: Mol. Biomolec. Spectrosc., 272, 120998 (2022).M. G. Fawzy, W. E. Hassan, A. A. Mostafa, R. A. Sayed, Spectrochim. Acta A: Mol. Biomolec. Spectrosc., 272, 120998 (2022).H. M. Lotfy, M. A. Hegazy, S. Mowaka, E. H. Mohamed, Spectrochim. Acta A: Mol. Biomolec. Spectrosc., 153, 321 (2016).H. M. Lotfy, M. A. Hegazy, S. Mowaka, E. H. Mohamed, Spectrochim. Acta A: Mol. Biomolec. Spectrosc., 153, 321 (2016).A. Gałuszka, Z. Migaszewski, J. Namieśnik, TrAC Trends Anal. Chem., 50, 78 (2013).A. Gałuszka, Z. Migaszewski, J. Namieśnik, TrAC Trends Anal. Chem., 50, 78 (2013).A. Ivanković, Int. J. Sust. Green Energy, 6, 39 (2017).A. Ivanković, Int. J. Sust. Green Energy, 6, 39 (2017).P. K. Naga, Y. Pragathi, S. Yenduri, Green Anal. Chem., 100182 (2024).P. K. Naga, Y. Pragathi, S. Yenduri, Green Anal. Chem., 100182 (2024).K. Naga Prashant, Y. Suvarna, H. N. Varalakshmi, Res. J. Pharm. Tech., 17, 6132 (2024).K. Naga Prashant, Y. Suvarna, H. N. Varalakshmi, Res. J. Pharm. Tech., 17, 6132 (2024).S. Yenduri, H. N. Varalakshmi, N. P. Koppuravuri, Indian J. Pharm. Ed. Res., 58, s543 (2024).S. Yenduri, H. N. Varalakshmi, N. P. Koppuravuri, Indian J. Pharm. Ed. Res., 58, s543 (2024).F. R. Mansour, J. Płotka-Wasylka, M. Locatelli, Analytica, 5, 451 (2024).F. R. Mansour, J. Płotka-Wasylka, M. Locatelli, Analytica, 5, 451 (2024).S. Yenduri, H. Sulthana, N. P. Koppuravuri, Green Anal. Chem., 6, 100074 (2023).S. Yenduri, H. Sulthana, N. P. Koppuravuri, Green Anal. Chem., 6, 100074 (2023).V. Mazzaracchio, A. Sassolini, K. Y. Mitra, D. Mitra, G. M. Stojanović, A. Willert, E. Sowade, R. R. Baumann, R. Zichner, D. Moscone, F. Arduini, Green Anal. Chem., 1, 100006 (2022).V. Mazzaracchio, A. Sassolini, K. Y. Mitra, D. Mitra, G. M. Stojanović, A. Willert, E. Sowade, R. R. Baumann, R. Zichner, D. Moscone, F. Arduini, Green Anal. Chem., 1, 100006 (2022).N. Manousi, W. Wojnowski, J. Płotka-Wasylka, V. Samanidou, Green Chem., 25, 7598 (2023).N. Manousi, W. Wojnowski, J. Płotka-Wasylka, V. Samanidou, Green Chem., 25, 7598 (2023).S. Yenduri, N. P. Koppuravuri, Green Anal. Chem., 9, 100108 (2024).S. Yenduri, N. P. Koppuravuri, Green Anal. Chem., 9, 100108 (2024).

The authors declare that there are no conflicts of interest present.