zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-2041Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESФизико-химические свойства и противогрибковая эффективность в отношении Candida albicans синтезированных с использованием экстракта миндаля биогенных наночастиц серебраBiogenic Silver Nanoparticles Synthesized Using Almond Extract: Physicochemical Properties and Antifungal Efficacy Against Candida albicansElywaM.ElywaM.

Загазиг

Mohammed Elywa

Zagazig

elywa2006@gmail.comAbd El-HamidM.Abd El-HamidM.

Загазиг

Marwa Abd El-Hamid

Zagazig

AdelT.AdelT.

Toqa Adel

Zagazig

HanafyM.HanafyM.

Загазиг

Magda Hanafy

Zagazig

Факультет естественных наук Университета ЗагазигаDepartment of Physics, Biophysics Branch, Faculty of Science, Zagazig UniversityФакультет ветеринарной медицины Университета ЗагазигаDepartment of Microbiology, Faculty of Veterinary Medicine, Zagazig University202526112025926829829Copyright © Elywa M., Abd El-Hamid M., Adel T., Hanafy M., 20252025Elywa M., Abd El-Hamid M., Adel T., Hanafy M.Elywa M., Abd El-Hamid M., Adel T., Hanafy M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/2041

Наночастицы серебра (НЧ Ag) синтезированы с использованием экстракта ядер миндаля в качестве природного восстановителя и стабилизатора и охарактеризованы с помощью спектрофотометрии в УФ-видимой области спектра, ИК-Фурье-спектроскопии, рентгеноструктурного анализа и просвечивающей электронной микроскопии. Размер частиц Ag NP, полученный с помощью всех методов, составил 18–23 нм. Показано, что синтезированные Ag NP проявляют умеренное цитотоксическое действие на нормальную линию фибробластов человека (BJ1), IC50 = 98.4 мкг/мл. Получены данные количественной ПЦР, оценивающей экспрессию двух генов вирулентности – ALS3 и HWP1. Показано, что синтезированные НЧ Ag подавляют экспрессию основных генов вирулентности Candida albicans более эффективно (в 1.5 раза для ALS3 и в 1.2 раза для HWP1), чем крем с миконазолом. In vivo показано, что НЧ Ag эффективно снижают грибковую нагрузку в инфицированных тканях кожи с течением времени.

Silver nanoparticles (Ag NPs) were synthesized by using the extract of almond kernels as a natural reducing and stabilizing agent. The synthesized Ag NPs were characterized by UV-Vis spectrophotometer, FTIR spectroscopy, XRD characterization, and TEM imaging. As a result, every technique showed that the particle size of Ag NPs was 18–23 nm. It also showed that the synthesized Ag NPs exhibited a moderate cytotoxic effect on normal human fibroblast cell line (BJ1), IC50 was 98.4 μg/mL. According to quantitative PCR (qPCR) data, which evaluate the expression of two virulence-related genes, ALS3 and HWP1, in Candida albicans, it showed that the synthesized Ag NPs suppresses the expression of the main virulence genes of Candida albicans more effectively (1.5 times for ALS3 and 1.2 times for HWP1) than miconazole cream. In vivo, in rats, it was shown that Ag NPs also effectively reduced the fungal load in infected skin tissues over time.

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The authors declare that there are no conflicts of interest present.