zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-2105Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESОценка антибактериальных и противораковых свойств “зеленых” наночастиц оксида цинка, синтезированных из листьев чая Camellia sinensisEvaluation of the Antibacterial and Anticancer Properties of Green Zinc Oxide Nanoparticles Synthesized from Spent Camellia Sinensis (Tea) LeavesIslamM. R.IslamM. R.

Ри-Бхой, Мегхалая

Department of Applied Biology

Ri-Bhoi, Meghalaya

AroraА.AroraA.

Гувахати, Ассам

Department of Biosciences and Bioengineering

Guwahati, Assam

LaskarI.LaskarI.

Ри-Бхой, Мегхалая

Department of Applied Biology

Ri-Bhoi, Meghalaya

SahaR.SahaR.

Ри-Бхой, Мегхалая

Department of Physics

Ri-Bhoi, Meghalaya

saharajib26@gmail.comУниверситет науки и технологийUniversity of Science and TechnologyИндийский технологический институт ГувахатиIndian Institute of Technology Guwahati202622012026931151151Copyright © Islam M.R., Arora А., Laskar I., Saha R., 20262026Islam M.R., Arora А., Laskar I., Saha R.Islam M.R., Arora A., Laskar I., Saha R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/2105

Наночастицы оксида цинка (НЧ ZnO) получены из отработанных листьев C. sinensis (чая), которые служили как восстановителем, так и стабилизатором. В качестве прекурсора для синтеза НЧ в экстрактах отработанных листьев C. sinensis использован дигидрат ацетата цинка [(CH3COO)2Zn × 2H2O]. Физико-химические характеристики НЧ ZnO определены с помощью УФ-видимой спектроскопии, ИК-Фурье-спектроскопии (FT-IR), сканирующей электронной микроскопии с полевой эмиссией (FE-SEM), энергодисперсионного рентгеновского спектрометра (EDX), рентгеновской дифракции (XRD), фотолюминесцентной спектроскопии и динамического рассеяния света. Синтезированные НЧ ZnO показали максимум поглощения в УФ-видимом диапазоне при 370 нм и гексагональную структуру вюрцита со средним размером кристаллитов 56 нм, что подтверждено методом XRD. FE-SEM подтвердил сферическую морфологию, анализ EDX показал, что цинк является преобладающим элементом, за которым следует кислород. Антибактериальная эффективность НЧ ZnO оценена в отношении как грамотрицательных, так и грамположительных патогенов. Показана оптимальная эффективность против Streptococcus pyogenes и Staphylococcus aureus. Анализ 3-(4,5-диметилтиазол-2-ил)-2,5-дифенилтетразолий бромида (MTT) показал, что НЧ ZnO проявляют зависимую от концентрации противораковую активность в отношении клеток HeLa с IC50 ~33.5 нм. Визуализация живых и мертвых клеток подтвердила 4.6-кратное и 3.5-кратное увеличение количества мертвых клеток при концентрациях IC50 через 24 и 48 ч. Обработка НЧ ZnO значительно повысила выработку активных форм кислорода. Это указывает на то, что их цитотоксичность опосредована генерацией активных форм кислорода и их летальными эффектами.

Plant-based food waste contains high concentrations of phytochemicals, rendering it an appealing candidate for the eco-friendly production of metal nanoparticles. In this study, zinc oxide nanoparticles (ZnO NPs) were prepared from spent C. sinensis (tea) leaves, serving as both a reducing and a stabilizing agent. Zinc acetate dihydrate [(CH3COO)2Zn × 2H2O] was used as a precursor in the spent C. sinensis leaf extracts for the NPs synthesis. The physicochemical characterization of ZnO NPs was characterized using ultraviolet–visible spectroscopy (UV-VIS), Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectrophotometer (EDX), X-ray diffraction (XRD), photoluminescence spectroscopy (PL), and dynamic light scattering (DLS). The synthesized ZnO NPs displayed a strong UV–Vis absorption peak at 370 nm, and a wurtzite hexagonal structure with an average crystalline size of 56 nm, as confirmed by XRD. The FESEM confirmed a spherical morphology, while EDX analysis indicated zinc as the predominant element, followed by oxygen. The antibacterial efficacy of ZnO NPs was assessed against both Gram-negative and Gram-positive pathogens, exhibiting optimal effectiveness against Streptococcus pyogenes and Staphylococcus aureus. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay revealed that ZnO NPs exhibited concentration-dependent anticancer activity against HeLa cells, with an IC50 of approximately 33.5 mM. Live–dead imaging confirmed a 4.6-fold and 3.5-fold increase in dead cells at IC50 concentrations after 24 and 48 h, respectively. Furthermore, treatment with ZnO NPs significantly elevated reactive oxygen species (ROS) production, indicating that their cytotoxicity is mediated through ROS generation and its lethal effects. Our findings suggest that ZnO NPs made from spent C. sinensis leaf extracts could have antibacterial and anticancer properties.

оксид цинкананочастицалистья чаяC. sinensisантибактериальные свойствапротивораковые свойстваzinc oxidenanoparticlespentC. sinensisantibacterialanticancerReferencesR. Farjana, Md. A. M. Patwary, Md. A. B. Siddique, B. M. Shahriar, Md. H. Aminul, A. Beauty, R. Rimi, Md. A. Haque, A. K. M. Royhan Uddin, Roy. Soc. Open Sci., 9, No. 11, 220858 (2022), http://doi.org/10.1098/rsos.220858.R. Farjana, Md. A. M. Patwary, Md. A. B. Siddique, B. M. Shahriar, Md. H. Aminul, A. Beauty, R. Rimi, Md. A. Haque, A. K. M. Royhan Uddin, Roy. Soc. Open Sci., 9, No. 11, 220858 (2022), http://doi.org/10.1098/rsos.220858.S. Chandrasekaran, V. Anbazhagan, S. Anusuya, Appl. Biochem. Biotechnol., 195, 3840–3854 (2023), https://doi.org/10.1007/s12010-022-03900-0.S. Chandrasekaran, V. Anbazhagan, S. Anusuya, Appl. Biochem. Biotechnol., 195, 3840–3854 (2023), https://doi.org/10.1007/s12010-022-03900-0.B. T. Delma, M. A. Princela, Y. Subbareddy, M. A. Malbi, S. L. Roselet, M. S. Treasa, M. C. Rao, J. Appl. Spectrosc., 91, 912–920 (2024), https://doi.org/10.1007/s10812-024-01799-5.B. T. Delma, M. A. Princela, Y. Subbareddy, M. A. Malbi, S. L. Roselet, M. S. Treasa, M. C. Rao, J. Appl. Spectrosc., 91, 912–920 (2024), https://doi.org/10.1007/s10812-024-01799-5.M. F. Zayed, O. M. Shalby, W. H. Eisa, S. M. El-Kousy, A. M. Eltorgoman, J. Appl. Spectrosc., 89, 381–390 (2022), https://doi.org/10.1007/s10812-022-01368-8.M. F. Zayed, O. M. Shalby, W. H. Eisa, S. M. El-Kousy, A. M. Eltorgoman, J. Appl. Spectrosc., 89, 381–390 (2022), https://doi.org/10.1007/s10812-022-01368-8.A. Sirelkhatim, S. Mahmud, A. Seeni, A. Kaus, N. H. M. Ann, L. C. Bakhori, S. K. M. Hasan, D. Mohamad, Nano Micro. Lett., 7, 219–242 (2015), https://doi.org/10.1007/s40820-015-0040-x.A. Sirelkhatim, S. Mahmud, A. Seeni, A. Kaus, N. H. M. Ann, L. C. Bakhori, S. K. M. Hasan, D. Mohamad, Nano Micro. Lett., 7, 219–242 (2015), https://doi.org/10.1007/s40820-015-0040-x.M. Chennimalai, V. Vijayalakshmi, T. S. Senthil, N. Sivakumar, Mater. Today., 47, 1842–1846 (2021), https://doi.org/10.1016/j.matpr.2021.03.409.M. Chennimalai, V. Vijayalakshmi, T. S. Senthil, N. Sivakumar, Mater. Today., 47, 1842–1846 (2021), https://doi.org/10.1016/j.matpr.2021.03.409.M. Muthu Kathija, M. S. M. Badhusha, V. Rama, Appl. Surf. Sci. Adv., 15, 100400 (2023), https://doi.org/10.1016/j.apsadv.2023.100400.M. Muthu Kathija, M. S. M. Badhusha, V. Rama, Appl. Surf. Sci. Adv., 15, 100400 (2023), https://doi.org/10.1016/j.apsadv.2023.100400.K. Sajini, P. Jayaprada, P. Pardhasaradhi, B. T. P. Madhav, M. C. Rao, D. Rama Sekhara Reddy, R. K. N. R. Manepalli, J. Appl. Spectrosc., 89, 1177–1184 (2023), https://doi.org/10.1007/s10812-023-01484-z.K. Sajini, P. Jayaprada, P. Pardhasaradhi, B. T. P. Madhav, M. C. Rao, D. Rama Sekhara Reddy, R. K. N. R. Manepalli, J. Appl. Spectrosc., 89, 1177–1184 (2023), https://doi.org/10.1007/s10812-023-01484-z.T. U. Rahman, S. Mukhtar, M. A. Zeb, W. Liaqat, J. Appl. Spectrosc., 89, 773–779 (2022), https://doi.org/10.1007/s10812-022-01424-3.T. U. Rahman, S. Mukhtar, M. A. Zeb, W. Liaqat, J. Appl. Spectrosc., 89, 773–779 (2022), https://doi.org/10.1007/s10812-022-01424-3.K. Ramasubbu, V. D. Rajeswari, Appl. Biochem. Biotechnol., 196, 2652–2672 (2024), https://doi.org/10.1007/s12010-023-04631-6.K. Ramasubbu, V. D. Rajeswari, Appl. Biochem. Biotechnol., 196, 2652–2672 (2024), https://doi.org/10.1007/s12010-023-04631-6.A. Umamaheswari, S. L. Prabu, S. A. John, A. Puratchikody, Biotechnol. Rep. (Amst.), 29, e00595 (2021), https://doi.org/10.1016/j.btre.2021.e00595.A. Umamaheswari, S. L. Prabu, S. A. John, A. Puratchikody, Biotechnol. Rep. (Amst.), 29, e00595 (2021), https://doi.org/10.1016/j.btre.2021.e00595.S. Gul, M. F. Maqbool, D. Zheng, Y. Li, M. Khan, T. Ma, Appl. Biochem. Biotechnol., 194, 4220–4243 (2022), https://doi.org/10.1007/s12010-022-03960-2.S. Gul, M. F. Maqbool, D. Zheng, Y. Li, M. Khan, T. Ma, Appl. Biochem. Biotechnol., 194, 4220–4243 (2022), https://doi.org/10.1007/s12010-022-03960-2.A. B. Mousa, R. Moawad, Y. Abdallah, M. Abdel-Rasheed, A. M. A. Zaher, Pharm. Res., 40, 2281–2290 (2023), https://doi.org/10.1007/s11095-023-03505-0.A. B. Mousa, R. Moawad, Y. Abdallah, M. Abdel-Rasheed, A. M. A. Zaher, Pharm. Res., 40, 2281–2290 (2023), https://doi.org/10.1007/s11095-023-03505-0.R. L. Kalyani, S. V. N. Pammi, P. N.Pallela, K. Vijay, S. P. Padavalaa, K. V. R. Murthy, Mater. Sci. Eng. C, 103, 109 (2019), https://doi.org/10.1016/j.msec.2019.109756.R. L. Kalyani, S. V. N. Pammi, P. N.Pallela, K. Vijay, S. P. Padavalaa, K. V. R. Murthy, Mater. Sci. Eng. C, 103, 109 (2019), https://doi.org/10.1016/j.msec.2019.109756.H. A. El-Refai, A. M. Saleh, S. I. A. Mohamed, A. F. Aboul Naser, R. A. Zaki, S. K. Gomaa, M. A. Hamed, Appl. Biochem. Biotechnol., 196, 5953–5973 (2024), https://doi.org/10.1007/s12010-023-04817-y.H. A. El-Refai, A. M. Saleh, S. I. A. Mohamed, A. F. Aboul Naser, R. A. Zaki, S. K. Gomaa, M. A. Hamed, Appl. Biochem. Biotechnol., 196, 5953–5973 (2024), https://doi.org/10.1007/s12010-023-04817-y.N. N. Son, V. M. Vu Minh Thanh, N. T. Huong, Chem. Select., 8, No. 45, e202303214 (2023), https://doi.org/10.1002/slct.202303214.N. N. Son, V. M. Vu Minh Thanh, N. T. Huong, Chem. Select., 8, No. 45, e202303214 (2023), https://doi.org/10.1002/slct.202303214.S. Hariharan, S. Chauhan, K. Velu, S. Dharmaraj, S. Ganesan, Appl. Biochem. Biotechnol., 195, 5823–5837 (2023), https://doi.org/10.1007/s12010-023-04348-6.S. Hariharan, S. Chauhan, K. Velu, S. Dharmaraj, S. Ganesan, Appl. Biochem. Biotechnol., 195, 5823–5837 (2023), https://doi.org/10.1007/s12010-023-04348-6.A. F. Saad, Y. A. Selim, M. Hamada, E. Mohammed, J. Appl. Spectrosc., 90, 1142–1148 (2023), https://doi.org/10.1007/s10812-023-01644-1.A. F. Saad, Y. A. Selim, M. Hamada, E. Mohammed, J. Appl. Spectrosc., 90, 1142–1148 (2023), https://doi.org/10.1007/s10812-023-01644-1.C. B. Ong, M. S. M. Annuar, Appl. Biochem. Biotechnol., 196, 7534–7553 (2024), https://doi.org/10.1007/s12010-024-04946-y.C. B. Ong, M. S. M. Annuar, Appl. Biochem. Biotechnol., 196, 7534–7553 (2024), https://doi.org/10.1007/s12010-024-04946-y.B. Naiel, M. Fawzy, M. W. A. Halmy, A. E. D. Mahmoud, Sci. Rep., 12, 20370 (2022), https://doi.org/10.1038/s41598-022-24805-2.B. Naiel, M. Fawzy, M. W. A. Halmy, A. E. D. Mahmoud, Sci. Rep., 12, 20370 (2022), https://doi.org/10.1038/s41598-022-24805-2.H. M. Abdelmigid, N. A. Hussien, A. A. Alyamani, M. M. Morsi, N. M. Al Sufyani, H. A. Kadi, Molecules, 27, No. 4, 1236 (2022), https://doi.org/10.3390/molecules27041236.H. M. Abdelmigid, N. A. Hussien, A. A. Alyamani, M. M. Morsi, N. M. Al Sufyani, H. A. Kadi, Molecules, 27, No. 4, 1236 (2022), https://doi.org/10.3390/molecules27041236.O. Y. Maslov, M. A. Komisarenko, Y. S. Kolisnyk, T. A. Kostina, J. Org. Pharm. Chem., 19, 28–33 (2021), https://doi.org/10.24959/ophcj.21.238177.O. Y. Maslov, M. A. Komisarenko, Y. S. Kolisnyk, T. A. Kostina, J. Org. Pharm. Chem., 19, 28–33 (2021), https://doi.org/10.24959/ophcj.21.238177.K. S. Ng, Z. M. Zin, N. M. Maidin, H. Mamat, N. H. Juhari, M. K. Zainol, Food Res., 5, No. 1, 65–71 (2021), https://doi.org/10.26656/fr.2017.5(1).311.K. S. Ng, Z. M. Zin, N. M. Maidin, H. Mamat, N. H. Juhari, M. K. Zainol, Food Res., 5, No. 1, 65–71 (2021), https://doi.org/10.26656/fr.2017.5(1).311.M. F. Sun, C. L. Jiang, Y. S. Kong, J. L. Luo, P. Yin, G. Y. Guo, Foods, 11, No. 10, 1425 (2022), https://doi.org/10.3390/foods11101425.M. F. Sun, C. L. Jiang, Y. S. Kong, J. L. Luo, P. Yin, G. Y. Guo, Foods, 11, No. 10, 1425 (2022), https://doi.org/10.3390/foods11101425.D. Wianowska, M. A. Olszowy-Tomczyk, Molecules, 28, No. 3, 1186 (2023), https://doi.org/10.3390%2Fmolecules2803118.D. Wianowska, M. A. Olszowy-Tomczyk, Molecules, 28, No. 3, 1186 (2023), https://doi.org/10.3390%2Fmolecules2803118.M. Esteki, N. Memarbashi, J. Simal-Gandara, J. Food Compos. Anal., 106, 104321(1–8) (2022), https://doi.org/10.1016/j.jfca.2021.104321.M. Esteki, N. Memarbashi, J. Simal-Gandara, J. Food Compos. Anal., 106, 104321(1–8) (2022), https://doi.org/10.1016/j.jfca.2021.104321.M. A. Brza, S. B. Aziz, H. Anuar, F. Ali, M. A. Elham, D. S. J. Mohammed, R. T. Abdulwahid, S. Al‑Zangana, Sci. Rep., 10, 18108 (2020), https://doi.org/10.1038/s41598-020-75138-x.M. A. Brza, S. B. Aziz, H. Anuar, F. Ali, M. A. Elham, D. S. J. Mohammed, R. T. Abdulwahid, S. Al‑Zangana, Sci. Rep., 10, 18108 (2020), https://doi.org/10.1038/s41598-020-75138-x.R. Devadharshini, S. Charulatha, A. Das, J. Bindhu, Int. J. Cur. Res. Rev., 13, No. 3 (2021), http://dx.doi.org/10.31782/IJCRR.2021.13333.R. Devadharshini, S. Charulatha, A. Das, J. Bindhu, Int. J. Cur. Res. Rev., 13, No. 3 (2021), http://dx.doi.org/10.31782/IJCRR.2021.13333.I. Nugrahani, M. Sundalian, Biointerface Res. Appl. Chem., 10, No. 1, 4721–4727 (2020), https://doi.org/10.33263/BRIAC10.1721727.I. Nugrahani, M. Sundalian, Biointerface Res. Appl. Chem., 10, No. 1, 4721–4727 (2020), https://doi.org/10.33263/BRIAC10.1721727.R. Md. Hasan, M. M. Haque, A. Md. Hoque, S. Sultana, M. M. Rahman, A. A. Md. Shaikh, K. U. Md. Sarker, Heliyon., 10, No. 1, e23514 (2024), https://doi.org/10.1016/j.heliyon.2023.e23514.R. Md. Hasan, M. M. Haque, A. Md. Hoque, S. Sultana, M. M. Rahman, A. A. Md. Shaikh, K. U. Md. Sarker, Heliyon., 10, No. 1, e23514 (2024), https://doi.org/10.1016/j.heliyon.2023.e23514.D. A. Bajrami, A. Ganiji, Z. Saiti-Musliji, S. Jordanovska, J. Food., 5, No. 4, 55–59 (2023), https://doi.org/10.24018/ejfood.2023.5.4.682.D. A. Bajrami, A. Ganiji, Z. Saiti-Musliji, S. Jordanovska, J. Food., 5, No. 4, 55–59 (2023), https://doi.org/10.24018/ejfood.2023.5.4.682.X. Xiao, B. Peng, L. Cai, X. Zhang, Sci. Rep., 8, 7571 (2018), https://doi.org/10.1038/s41598-018-26022-2.X. Xiao, B. Peng, L. Cai, X. Zhang, Sci. Rep., 8, 7571 (2018), https://doi.org/10.1038/s41598-018-26022-2.P. Ramesh, K. Saravanan, P. Manogar, J. Johnson, E. Vinoth, M. Mayakannan, Bio-Sens. Res., 31, 100399 (2021), https://doi.org/10.1016/j.sbsr.2021.100399.P. Ramesh, K. Saravanan, P. Manogar, J. Johnson, E. Vinoth, M. Mayakannan, Bio-Sens. Res., 31, 100399 (2021), https://doi.org/10.1016/j.sbsr.2021.100399.S. Alamdari, M. S. Ghamsari, C. Lee, W. Han, H.-H. Park, M. J. Tafreshi, H. Afarideh, M. H. M. Ara, Appl. Sci., 10, No. 10, 3620 (2020), https://doi.org/10.3390/app10103620.S. Alamdari, M. S. Ghamsari, C. Lee, W. Han, H.-H. Park, M. J. Tafreshi, H. Afarideh, M. H. M. Ara, Appl. Sci., 10, No. 10, 3620 (2020), https://doi.org/10.3390/app10103620.R. Álvarez-Chimal, V. I. García-Pérez, M. A. Álvarez-Pérez, J. A. Arenas-Alator, Mater. Sci. Eng., C118, 1115 (2021), https://doi.org/10.1016/j.msec.2020.111540.R. Álvarez-Chimal, V. I. García-Pérez, M. A. Álvarez-Pérez, J. A. Arenas-Alator, Mater. Sci. Eng., C118, 1115 (2021), https://doi.org/10.1016/j.msec.2020.111540.A. Jayachandran, T. R. Aswathy, A. S. Nair, Biochem. Biophys. Rep., 26, 100995 (2021), https://doi.org/10.1016/j.bbrep.2021.100995.A. Jayachandran, T. R. Aswathy, A. S. Nair, Biochem. Biophys. Rep., 26, 100995 (2021), https://doi.org/10.1016/j.bbrep.2021.100995.W. Muhammad, N. Ullah, M. Muhammad Haroona, B. H. Abbasi, RSC Adv., 9, 29541–29548 (2019), https://doi.org/10.1039/C9RA04424H.W. Muhammad, N. Ullah, M. Muhammad Haroona, B. H. Abbasi, RSC Adv., 9, 29541–29548 (2019), https://doi.org/10.1039/C9RA04424H.A. Dey, S. Somaiah, Microsc. Res. Tech., 85, No. 8, 2835–2847 (2022), https://doi.org/10.1002/jemt.24132.A. Dey, S. Somaiah, Microsc. Res. Tech., 85, No. 8, 2835–2847 (2022), https://doi.org/10.1002/jemt.24132.A. Iqbal, A. ul Haq, L. Rios-Aspajo, A. Iturriaga-Chavez, Global NEST. J., 25, No. 9, 150–158 (2023), https://doi.org/10.30955/gnj.005285.A. Iqbal, A. ul Haq, L. Rios-Aspajo, A. Iturriaga-Chavez, Global NEST. J., 25, No. 9, 150–158 (2023), https://doi.org/10.30955/gnj.005285.S. Raha, Md. Ahmaruzzaman, Nanoscale Adv., 4, 1868–1925 (2022), https://doi.org/10.1039/D1NA00880C.S. Raha, Md. Ahmaruzzaman, Nanoscale Adv., 4, 1868–1925 (2022), https://doi.org/10.1039/D1NA00880C.M. Naseer, U. Aslam, B. Khalid, B. Chen, Sci. Rep., 10, 9055 (2020), https://doi.org/10.1038/s41598-02065949-3.M. Naseer, U. Aslam, B. Khalid, B. Chen, Sci. Rep., 10, 9055 (2020), https://doi.org/10.1038/s41598-02065949-3.J. V. González-Fernández, D. D. Pinzón-Moreno, A. A. Neciosup-Puican, M. V. Carranza-Oropez, J. Renew. Mater., 10, No. 3, 833–847 (2022), https://doi.org/10.32604/jrm.2021.017377.J. V. González-Fernández, D. D. Pinzón-Moreno, A. A. Neciosup-Puican, M. V. Carranza-Oropez, J. Renew. Mater., 10, No. 3, 833–847 (2022), https://doi.org/10.32604/jrm.2021.017377.E. Tilahun, Y. Adimasu, Y. Dessie, ACS Omega, 8, No. 30, 27344–27354 (2023), https://doi.org/10.1021/acsomega.3c02709.E. Tilahun, Y. Adimasu, Y. Dessie, ACS Omega, 8, No. 30, 27344–27354 (2023), https://doi.org/10.1021/acsomega.3c02709.P. Perumal, N. A. Sathakkathulla, K. Kumaran, R. Ravikumar, J. J. Selvaraj, V. Nagendran, M. Gurusamy, N. Shaik, S. G. Prabhakaran, V. S. Palanichamy, V. Ganesan, P. P. Thiraviam, S. Gunalanand, S. Rathinasamy, Sci. Rep., 14, 2204 (2024), https://doi.org/10.1038/s41598-024-52217-x.P. Perumal, N. A. Sathakkathulla, K. Kumaran, R. Ravikumar, J. J. Selvaraj, V. Nagendran, M. Gurusamy, N. Shaik, S. G. Prabhakaran, V. S. Palanichamy, V. Ganesan, P. P. Thiraviam, S. Gunalanand, S. Rathinasamy, Sci. Rep., 14, 2204 (2024), https://doi.org/10.1038/s41598-024-52217-x.A. S. Al Rahbi, A. H. Al Mawali, S. S. Al Rawahi, R. K. Al Dighishi, F. A. Al Abri, A. Ahmed, S. Rahman, Water Pract. Technol., 19, No. 4, 1219–1231 (2024), https://doi.org/10.2166/wpt.2024.042.A. S. Al Rahbi, A. H. Al Mawali, S. S. Al Rawahi, R. K. Al Dighishi, F. A. Al Abri, A. Ahmed, S. Rahman, Water Pract. Technol., 19, No. 4, 1219–1231 (2024), https://doi.org/10.2166/wpt.2024.042.R. Saemi, E. Taghavi, H. Jafarizadeh-Malmiri, N. Anarjan, Green Process Synth., 10, No. 1, 112–124 (2021), https://doi.org/10.1515/gps-2021-0011.R. Saemi, E. Taghavi, H. Jafarizadeh-Malmiri, N. Anarjan, Green Process Synth., 10, No. 1, 112–124 (2021), https://doi.org/10.1515/gps-2021-0011.D. Kamarajan, B. Anburaj, V. Porkalai, A. Muthuvel, G. Nedunchezhian, N. Mahendran, J. Water Environ. Nanotechnol., 7, No. 2, 180–193 (2022), https://doi.org/10.22090/jwent.2022.02.006.D. Kamarajan, B. Anburaj, V. Porkalai, A. Muthuvel, G. Nedunchezhian, N. Mahendran, J. Water Environ. Nanotechnol., 7, No. 2, 180–193 (2022), https://doi.org/10.22090/jwent.2022.02.006.A. Sharma, R. Nagraik, S. Sharma, G. Sharma, S. Pandey, S. Azizov, P. K. Chauhan, D. Kumar, Results Chem., 4, 100509 (2022), https://doi.org/10.1016/j.rechem.2022.100509.A. Sharma, R. Nagraik, S. Sharma, G. Sharma, S. Pandey, S. Azizov, P. K. Chauhan, D. Kumar, Results Chem., 4, 100509 (2022), https://doi.org/10.1016/j.rechem.2022.100509.R. S. Brishti, M. A. Habib, M. H. Ara, K. M. R. Karim, M. K. Islam, J. Naime, M. M. H. Hasan Rumonand, M. A. R. Khan, Results Chem., 7, 101441 (2024), https://doi.org/10.1016/j.rechem.2024.101441.R. S. Brishti, M. A. Habib, M. H. Ara, K. M. R. Karim, M. K. Islam, J. Naime, M. M. H. Hasan Rumonand, M. A. R. Khan, Results Chem., 7, 101441 (2024), https://doi.org/10.1016/j.rechem.2024.101441.F. N. Alharbi, Z. M. Abaker, S. Z. A. Makawi, Inorganics, 11, No. 8, 328 (2023), https://doi.org/10.3390/inorganics11080328.F. N. Alharbi, Z. M. Abaker, S. Z. A. Makawi, Inorganics, 11, No. 8, 328 (2023), https://doi.org/10.3390/inorganics11080328.S. Chakraborty, S. Majumder, A. Ghosh, S. Saha, M. Bhattacharya, Bull. Natl. Res. Cent., 45, 48 (2021), https://doi.org/10.1186/s42269-021-00503-3.S. Chakraborty, S. Majumder, A. Ghosh, S. Saha, M. Bhattacharya, Bull. Natl. Res. Cent., 45, 48 (2021), https://doi.org/10.1186/s42269-021-00503-3.S. M. Ghamsari, S. Alamdari, W. Han, H.Park, Int. J. Nanomedicine, 12, 207–216 (2017), https://doi.org/10.2147/IJN.S118637.S. M. Ghamsari, S. Alamdari, W. Han, H.Park, Int. J. Nanomedicine, 12, 207–216 (2017), https://doi.org/10.2147/IJN.S118637.S. Umavathi, S. Mahboob, M. Govindarajan, K. A. Al-Ghanim, Z. Ahmed, P. Virik, N. Al-Mulhm, M. Subash, K. Gopinath, C. Kavitha, Saudi J. Biol. Sci., 28, No. 3, 1808–1815 (2021), https://doi.org/10.1016/j.sjbs.2020.12.025.S. Umavathi, S. Mahboob, M. Govindarajan, K. A. Al-Ghanim, Z. Ahmed, P. Virik, N. Al-Mulhm, M. Subash, K. Gopinath, C. Kavitha, Saudi J. Biol. Sci., 28, No. 3, 1808–1815 (2021), https://doi.org/10.1016/j.sjbs.2020.12.025.A. M. Abdo, A. Fouda, A. M. Eid, N. M. Fahmy, A. M. Elsayed, A. M. A. Khalil, O. M. Alzahrani, A. F. Ahmed, A. M. Soliman, Materials (Basel), 14, No. 22, 6983 (2021), https://doi.org/10.3390/ma14226983.A. M. Abdo, A. Fouda, A. M. Eid, N. M. Fahmy, A. M. Elsayed, A. M. A. Khalil, O. M. Alzahrani, A. F. Ahmed, A. M. Soliman, Materials (Basel), 14, No. 22, 6983 (2021), https://doi.org/10.3390/ma14226983.E. E. Imade, T. O. Ajiboye, A. E. Fadiji, D. C. Onwudiwe, O. O. Babalola, Sci. Afr., 16, e01152 (2022), https://doi.org/10.1016/j.sciaf.2022.e01152.E. E. Imade, T. O. Ajiboye, A. E. Fadiji, D. C. Onwudiwe, O. O. Babalola, Sci. Afr., 16, e01152 (2022), https://doi.org/10.1016/j.sciaf.2022.e01152.

The authors declare that there are no conflicts of interest present.