zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-2165Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESКоличественное определение ципрофлоксацина методом ядерно-магнитной резонансной спектроскопии в бездейтериевой средеCiprofloxacin Quantification with Nuclear Magnetic Resonance Spectroscopy in a Deuterium-Free EnvironmentAzamM.AzamM.

Muhammad Azam - Department of Chemistry, University of Okara.

azamshah71@yahoo.comAltafA. A.AltafA. A.

Университет Окара; Институт химии, Университет инженерных и информационных технологий Хваджи Фарида.

Рахим-Яр-Хан

Ataf Ali Altaf - Department of Chemistry, University of Okara; Institute of Chemistry, Khwaja Fareed University of Engineering and Information Technology.

Rahim Yar Khan

atafali_altaf@uo.edu.pkHamayunM.HamayunM.

Muhammad Hamayun - Department of Chemistry, University of Gujrat.

hamayunf@uog.edu.pkУниверситет ОкараUniversity of OkaraУниверситет Окара; Университет инженерных и информационных технологий Хваджи ФаридаUniversity of Okara; Khwaja Fareed University of Engineering and Information TechnologyУниверситет ГуджратаUniversity of Gujrat2026300320269322887Copyright © Azam M., Altaf A.A., Hamayun M., 20262026Azam M., Altaf A.A., Hamayun M.Azam M., Altaf A.A., Hamayun M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/2165

Недейтерированный растворитель (H2O) использован для количественного определения ципрофлоксацина (ЦИП) методом количественной ядерно-магнитной резонансной спектроскопии (ЯМР). В качестве внутреннего стандарта для решения задачи абсолютного интегрирования сигналов в спектрах ЯМР использован п-нитрофенол. Результаты количественного определения сравнивались с полученными методами высокоэффективной жидкостной хроматографии (ВЭЖХ) и анализировались с помощью дисперсионного анализа. Показано, что регрессионная модель является значимой (F = 37515.62, p < 0.001), а фактическое количество аналита является мощным предиктором количественной концентрации. Результаты дисперсионного анализа (ANOVA) показали, что для количественного анализа ципрофлоксацина использование простой воды в качестве растворителя в методе количественной ЯМР-спектроскопии столь же эффективно, как и метод ВЭЖХ. Рассматриваемый метод компенсирует недостатки метода ВЭЖХ для количественного измерения ципрофлоксацина, а использование п-нитрофенола позволяет преодолеть проблему абсолютного интегрирования сигналов, связанных со спектрами ЯМР.

The quantitative nuclear magnetic resonance (qNMR) method of quantifying the content of pharmaceuticals is an important and growing science in the current era. The deuterium-based solvents contribute to the main costs of the NMR-based analysis. In this study, we introduced the use of deuterium-free solvent (i.e., water H2O) for the quantification of ciprofloxacin (CIP) using the qNMR method. p-Nitrophenol (PNP) was used as an internal standard to solve the absolute integration of signals in NMR spectra. Quantification results were compared with literature-reported high-performance liquid chromatographic (HPLC) methods and analyzed by analysis of variance (ANOVA). The ANOVA results show that the regression model is highly significant (F = 37515.62, p < 0.001), indicating that the actual quantity of the analyte is a powerful predictor of the quantized concentration. An ANOVA study demonstrated that using simple water as a solvent in the qNMR method is as effective as the HPLC method for the quantitative analysis of CIP. The investigated method accurately compensated for the deficiencies of the current HPLC method for quantitative measurement of CIP, and the use of PNP overcame the problem of absolute integration of signals associated with NMR spectra.

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The authors declare that there are no conflicts of interest present.