zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-2170Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESФлуоресцентный зонд для обнаружения Cu2+ в клетках с хорошей биосовместимостью и селективностьюNovel Fluorescence Probe with Good Biocompatibility and Selectivity for the Detection of Cu2+ in CellsZhaoX.ZhaoX.

Школа культурного наследия.

Сиань

Xing Zhao - School of Cultural Heritage, Northwest University.

Xi’an

ZhangP.ZhangP.

Школа культурного наследия.

Сиань

Peiyue Zhang - School of Cultural Heritage, Northwest University.

Xi’an

LengX.LengX.

Колледж биологических наук, Северо-Западный университет; Колледж химии и материаловедения, Северо-Западный университет.

Сиань

Xin Leng - College of Life Sciences, Northwest University; College of Chemistry & Materials Science, Northwest University.

Xi’an

lengxin@nwu.edu.cnLiuJ.LiuJ.

Колледж химии и материаловедения.

Сиань

Jing Liu - College of Chemistry & Materials Science, Northwest University.

Xi’an

ZhangY.ZhangY.

Колледж химии и материаловедения.

Сиань

Yang Zhang - College of Chemistry & Materials Science, Northwest University.

Xi’an

BaiY.BaiY.

Колледж химии и материаловедения.

Сиань

Yinjuan Bai - College of Chemistry & Materials Science, Northwest University.

Xi’an

YangB.YangB.

Колледж химии и материаловедения.

Сиань

Bingqin Yang - College of Chemistry & Materials Science, Northwest University.

Xi’an

Северо-Западный университетNorthwest University2026300320269322918Copyright © Zhao X., Zhang P., Leng X., Liu J., Zhang Y., Bai Y., Yang B., 20262026Zhao X., Zhang P., Leng X., Liu J., Zhang Y., Bai Y., Yang B.Zhao X., Zhang P., Leng X., Liu J., Zhang Y., Bai Y., Yang B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/2170

Разработан и охарактеризован обратимый флуоресцентный зонд со специфическим распознаванием Cu2⁺ для обнаружения клеток и биовизуализации. По сравнению с другими катионами в растворе, зонд продемонстрировал исключительную селективность и чувствительность к Cu2⁺. Взаимодействие между зондом и Cu2⁺ ускорило процесс раскрытия кольца, что привело к появлению новой полосы излучения при 525 нм в определенном диапазоне pH. Обнаруженная сильная линейная зависимость между интенсивностью флуоресценции и концентрацией Cu2⁺ в диапазоне 0.0–1.0 эквивалента с пределом обнаружения 4.52 мкМ. Зависимости Джоба и анализ ИК-спектра показали, что зонд образует комплекс с Cu2⁺ в соотношении 1:1. Показано также, что зонд обладает хорошей биосовместимостью и может эффективно использоваться для визуализации клеток на наличие Cu2⁺. На основании этого можно разработать и изготовить обратимые флуоресцентные зонды, специфичные для Cu2⁺, с хорошими характеристиками флуоресцентного отклика и объединить их с технологией флуоресцентной спектроскопии для точного количественного определения Cu2⁺ в сложных образцах.

Trace element analysis of ancient human remains plays an important role in reconstructing dietary habits and living environments. However, conventional analytical methods have critical limitations such as destructive sampling, high costs, and inadequate sensitivity. To address these challenges, a reversible fluorescent probe with specific recognition for Cu2⁺ was fabricated and characterized in this study for cell detection and bioimaging. Compared with other cations in solution, the probe exhibited exceptional selectivity and sensitivity towards Cu2⁺. The interaction between the probe and Cu2⁺ accelerated the ring-opening process, resulting in a new emission band at 525 nm within a specific pH range. A strong linear relationship between the fluorescence intensity and Cu2⁺ concentration, ranging from 0.0 to 1.0 equivalents, with a detection limit of 4.52 μM, was observed. In addition, Job plots and infrared spectral analysis revealed that B probe complexed Cu2+ at a 1:1 ratio. Biological testing revealed that the probe has good biocompatibility and can be effectively used for Cu2⁺ cell imaging. Cu2+-specific reversible fluorescent probes can be designed and fabricated with good fluorescence response characteristics and combined with fluorescence spectroscopy technology to accurately quantify Cu2+ in complex ancient human samples.

флуоресцентный зондмикроэлементобнаружениебиосовместимостьfluorescent probetrace elementdetectionbiocompatibilityThis research was funded by the Shaanxi Provincial Natural Science Foundation funds research projects in basic scientific areas (grant No. 2024JC-YBMS-135), the National Natural Science Foundation of China (grant No. 52203126), the Xi and Natural Science Foundation (grant No. 2026JH-SZRJ-0272), the Archaeological Talent Promotion Program of China (grant No. 2025-207), and the Major Science and Technology Project of Gansu Province (grant No. 25ZDFA014). The authors acknowledge Fulin Chen, Northwest University, for his help in interpreting the significance of the results obtained from the current study.ReferencesSijia Xiang, Yangzhong Liu, J. Univ. Chem., 37, No. 3, 7–13 (2022).Sijia Xiang, Yangzhong Liu, J. Univ. Chem., 37, No. 3, 7–13 (2022).R. A. Festa, D. J. Thiele, Current Biology, 21, No. 21, R877–R883 (2011).R. A. Festa, D. J. 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The authors declare that there are no conflicts of interest present.