zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-306Research Article******СПЕКТРОСКОПИЧЕСКОЕ ИССЛЕДОВАНИЕ ВЗАИМОДЕЙСТВИЯ МЕЖДУ КОМПЛЕКСОМ КРАСИТЕЛЬ БИСМАРКА КОРИЧНЕВЫЙ R-Eu(III) И ДНКA SPECTROSCOPIC STUDY OF THE INTERACTION BETWEEN THE BISMARCK BROWN R-Eu(III) COMPLEX AND DNALiH. .LiH. .lihongbo112@126.comTangY. .TangY. .noemail@neicon.ruLeiSh. .LeiSh. .noemail@neicon.ruYouZ. .YouZ. .noemail@neicon.ruYangT. .YangT. .noemail@neicon.ruWangS. .WangS. .noemail@neicon.ruКолледж химии и химической инженерии, Цзянсиский педагогический университетCollege of Chemistry and Chemical Engineering, Jiangxi Normal University201810032020854688(1)-688(9)Copyright © Li H..., Tang Y..., Lei S..., You Z..., Yang T..., Wang S..., 20202020Li H..., Tang Y..., Lei S..., You Z..., Yang T..., Wang S...Li H..., Tang Y..., Lei S..., You Z..., Yang T..., Wang S...Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/306

C использованием акридинового оранжевого (АО) зонда для спектроскопии поглощения УФ излучения и флуоресцентной спектроскопии изучено взаимодействие комплекса Eu(III)-BBR с ДНК, выделенной из молок сельди. Показано, что как АО, так и комплекс Eu(III)-BBR могут интеркалировать в пары оснований ДНК. Константы связывания комплекса Eu(III)-BBR с ДНК Kθ298.15K = = 1.58×104 и Kθ308.15K = 9.35×104 л/моль, при этом процесс связывания управляется энтропией. Показано, что моды взаимодействия между комплексом Eu(III)-BBR и ДНК включают связывание канавок и интеркалирующее связывание. Механизм взаимодействия между комплексом Eu(III)-BBR- и ДНК является теоретической основой для синтеза новых лекарств.

The interaction of the Eu(III)-BBR complex with herring sperm DNA is investigated using an acridine orange (AO) probe for UV-vis absorption spectroscopy and fluorescence spectroscopy. The results indicate that both the AO probe and the Eu(III)-BBR complex can intercalate into the base pairs of DNA. The binding constants of the Eu(III)-BBR complex with DNA are Kθ298.15K = 1.58 × 104 L/mol and Kθ308.15K = 9.35 × 104 L/mol, and the binding process is entropy-driven. It is shown that the interaction modes between the Eu(III)-BBR complex and DNA include groove binding and intercalative binding. The mechanism of the interaction between the Eu(III)-BBR complex and DNA will provide an important theoretical basis for the synthesis of new drugs.

краситель Бисмарка коричневый REu(III)ДНКакридиновый оранжевыймода взаимодействияBismarck brown REu(III)DNAacridine orangeinteraction modeReferencesG. Zhang, J. Guo, N. Zhao, J. Wang, Sens. Actuat. B: Chem., 144, No. 1, 239-246 (2010).G. Zhang, J. Guo, N. Zhao, J. Wang, Sens. Actuat. B: Chem., 144, No. 1, 239-246 (2010).B. R. You, S. Z. Kim, S. H. Kim, W. H. Park, Mol. Cell. Biochem., 357, No. 1, 295-303 (2011).B. R. You, S. Z. Kim, S. H. Kim, W. H. Park, Mol. Cell. Biochem., 357, No. 1, 295-303 (2011).D. K. Maurya, N. Nandakumar, T. P. A. Devasagayam, J. Clin. Biochem. Nutr., 48, No. 1, 85-90 (2010).D. K. Maurya, N. Nandakumar, T. P. A. Devasagayam, J. Clin. Biochem. Nutr., 48, No. 1, 85-90 (2010).A. Faried, D. Kurnia, L. S. Faried, N. Usman, T. Miyazaki, H. Kato, H. Kuwano, Int. J. Oncol., 30, No. 3, 605-613 (2007).A. Faried, D. Kurnia, L. S. Faried, N. Usman, T. Miyazaki, H. Kato, H. Kuwano, Int. J. Oncol., 30, No. 3, 605-613 (2007).J. D. Hsu, S. H. Kao, T. T. Ou, Y. J. Chen, Y. J. Li, C. J. Wang, J. Agric. Food Chem., 59, No. 5, 1996-2003 (2011).J. D. Hsu, S. H. Kao, T. T. Ou, Y. J. Chen, Y. J. Li, C. J. Wang, J. Agric. Food Chem., 59, No. 5, 1996-2003 (2011).M. López-Lázaro, J. M. Calderón-Montaño, E. Burgos-Morón, C. A. Austin, Mutagenesis, 26, No. 4, 489-498 (2011).M. López-Lázaro, J. M. Calderón-Montaño, E. Burgos-Morón, C. A. Austin, Mutagenesis, 26, No. 4, 489-498 (2011).G. Zhang, J. Guo, J. Pan, X. Chen, J. Wang, J. Mol. Struct., 923, No. 1-3, 114-119 (2009).G. Zhang, J. Guo, J. Pan, X. Chen, J. Wang, J. Mol. Struct., 923, No. 1-3, 114-119 (2009).X. Q. He, Q. Y. Lin, R. D. Hu, X.-H. Lu, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 68, No. 1, 184-190 (2007).X. Q. He, Q. Y. Lin, R. D. Hu, X.-H. Lu, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 68, No. 1, 184-190 (2007).G. J. Chen, X. Qiao, C. Y. Gao, G. J. Xu, Z. L. Wang, J. L. Tian, J. Y. Xu, W. Gu, X. Liu, S.-P. Yan, J. Inorg. Biochem., 109, 90-96 (2012).G. J. Chen, X. Qiao, C. Y. Gao, G. J. Xu, Z. L. Wang, J. L. Tian, J. Y. Xu, W. Gu, X. Liu, S.-P. Yan, J. Inorg. Biochem., 109, 90-96 (2012).D. H. Tjahjono, T. Akutsu, N. Yoshioka, H. Inoue, Biochim. Biophys. Acta (BBA) - Gen. Subjects, 1472, No. 1-2, 333-343 (1999).D. H. Tjahjono, T. Akutsu, N. Yoshioka, H. Inoue, Biochim. Biophys. Acta (BBA) - Gen. Subjects, 1472, No. 1-2, 333-343 (1999).F. Arjmand, S. Parveen, M. Afzal, M. Shahid, J. Photochem. Photobiol. B: Biol., 114, 15-26 (2012). 688-9F. Arjmand, S. Parveen, M. Afzal, M. Shahid, J. Photochem. Photobiol. B: Biol., 114, 15-26 (2012). 688-9Y. Li, Z. Y. Yang, M. F. Wang, Eur. J. Med. Chem., 44, No. 11, 4585-4595 (2009).Y. Li, Z. Y. Yang, M. F. Wang, Eur. J. Med. Chem., 44, No. 11, 4585-4595 (2009).R. Hu, Q. Lin, W. Huang, Q. Yu, Rare Earths, 23, No. 3, 372-376 (2005).R. Hu, Q. Lin, W. Huang, Q. Yu, Rare Earths, 23, No. 3, 372-376 (2005).Z. A. Taha, A. M. Ajlouni, W. Al Momani, A. A. Al-Ghzawi, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 81, No. 1, 570-577 (2011).Z. A. Taha, A. M. Ajlouni, W. Al Momani, A. A. Al-Ghzawi, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 81, No. 1, 570-577 (2011).X. B. Li, A.R. Zhou, W. H. Yu, X. A. Chen, Rare Earths, 18, 2, 156-159 (2000).X. B. Li, A.R. Zhou, W. H. Yu, X. A. Chen, Rare Earths, 18, 2, 156-159 (2000).V. Trusova, A. Yudintsev, L. Limanskaya, G. Gorbenko, T. Deligeorgiev, J. Fluoresc., 23, No. 1, 193-202 (2013).V. Trusova, A. Yudintsev, L. Limanskaya, G. Gorbenko, T. Deligeorgiev, J. Fluoresc., 23, No. 1, 193-202 (2013).H. A. Azab, S. S. Al-Deyab, Z. M. Anwar, R. G. Ahmed, J. Chem. Eng. Data, 56, No. 4, 833-849 (2011).H. A. Azab, S. S. Al-Deyab, Z. M. Anwar, R. G. Ahmed, J. Chem. Eng. Data, 56, No. 4, 833-849 (2011).M. Taha, I. Khan, J. A. P. Coutinho, J. Inorg. Biochem., 157, 25-33 (2016).M. Taha, I. Khan, J. A. P. Coutinho, J. Inorg. Biochem., 157, 25-33 (2016).F. H. Hussein, M. H. Obies, A. A. A. Drea, Int. J. Chem. Sci., 8, No. 4, 2763-2774 (2010).F. H. Hussein, M. H. Obies, A. A. A. Drea, Int. J. Chem. Sci., 8, No. 4, 2763-2774 (2010).M. W. Girolami, R. W. Rousseau, J. Cryst. Growth, 71, No. 1, 220-224 (1985).M. W. Girolami, R. W. Rousseau, J. Cryst. Growth, 71, No. 1, 220-224 (1985).Y. Miao, Y. Li, Z. Zhang, G. Yan, Y. Bi, Analyt. Biochem., 475, 32-39 (2015).Y. Miao, Y. Li, Z. Zhang, G. Yan, Y. Bi, Analyt. Biochem., 475, 32-39 (2015).E. B. Brauns, C. J. Murphy, M. A. Berg, J. Am. Chem. Soc., 120, No. 10, 2449-2456 (1998).E. B. Brauns, C. J. Murphy, M. A. Berg, J. Am. Chem. Soc., 120, No. 10, 2449-2456 (1998).R. Christian, K. Thomas, Biochem., 17, No. 23, 4845-4854 (1978).R. Christian, K. Thomas, Biochem., 17, No. 23, 4845-4854 (1978).Y. Ni, S. Du, S. Kokot, Anal. Chim. Acta, 584, No. 1, 19-27 (2007).Y. Ni, S. Du, S. Kokot, Anal. Chim. Acta, 584, No. 1, 19-27 (2007).L. X. Xiao, N. Zhao, X. M. Wang, Lumin., 31, No. 1, 210-216 (2016).L. X. Xiao, N. Zhao, X. M. Wang, Lumin., 31, No. 1, 210-216 (2016).S. Clerc, Y. Barenholz, Anal. Biochem., 259, No. 1, 104-111 (1998).S. Clerc, Y. Barenholz, Anal. Biochem., 259, No. 1, 104-111 (1998).A. I. Kononov, E. B. Moroshkina, N. V. Tkachenko, H. Lemmetyinen, J. Phys. Chem. B, 105, No. 2, 535-541 (2001).A. I. Kononov, E. B. Moroshkina, N. V. Tkachenko, H. Lemmetyinen, J. Phys. Chem. B, 105, No. 2, 535-541 (2001).M. B. Lyles, I. L. Cameron, Biophys. Chem., 96, No. 1, 53-76 (2002).M. B. Lyles, I. L. Cameron, Biophys. Chem., 96, No. 1, 53-76 (2002).N. K. Modukuru, K. J. Snow, B. S. Perrin Jr., A. Bhambhani, M. Duff, C. V. Kumar, J. Photochem. Photobiol. A: Chem., 177, No. 1, 43-54 (2006).N. K. Modukuru, K. J. Snow, B. S. Perrin Jr., A. Bhambhani, M. Duff, C. V. Kumar, J. Photochem. Photobiol. A: Chem., 177, No. 1, 43-54 (2006).X. Ling, W. Zhong, Q. Huang, K. Ni, J. Photochem. Photobiol. B: Biol., 93, No. 3, 172-176 (2008).X. Ling, W. Zhong, Q. Huang, K. Ni, J. Photochem. Photobiol. B: Biol., 93, No. 3, 172-176 (2008).Mudasir, N. Yoshioka, H. Inoue, J. Inorg. Biochem., 77, No. 3-4, 239-247 (1999).Mudasir, N. Yoshioka, H. Inoue, J. Inorg. Biochem., 77, No. 3-4, 239-247 (1999).A. Kapur, J. L. Beck, M. M. Sheil, Rapid Commun. Mass Spectrom., 13, No. 24, 2489-2497 (1999).A. Kapur, J. L. Beck, M. M. Sheil, Rapid Commun. Mass Spectrom., 13, No. 24, 2489-2497 (1999).X. Jiang, X. Lin, Bioelectrochemistry, 68, No. 2, 206-212 (2006).X. Jiang, X. Lin, Bioelectrochemistry, 68, No. 2, 206-212 (2006).S. Q. Liu, M. L. Cao, S. L. Dong, Bioelectrochemistry, 74, No. 1, 164-169 (2008).S. Q. Liu, M. L. Cao, S. L. Dong, Bioelectrochemistry, 74, No. 1, 164-169 (2008).M. Carcelli, P. Mazza, C. Pelizzi, F. Zani, J. Inorg. Biochem., 57, No. 1, 43-62 (1995).M. Carcelli, P. Mazza, C. Pelizzi, F. Zani, J. Inorg. Biochem., 57, No. 1, 43-62 (1995).S. Bi, C. Qiao, D. Song, Y. Tian, D. Gao, Y. Sun, H. Zhang, Sens. Actuat. B: Chem., 119, No. 1, 199-208 (2006).S. Bi, C. Qiao, D. Song, Y. Tian, D. Gao, Y. Sun, H. Zhang, Sens. Actuat. B: Chem., 119, No. 1, 199-208 (2006).S. Bi, H. Zhang, C. Qiao, Y. Sun, C. Liu, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 69, No. 1, 123-129 (2008).S. Bi, H. Zhang, C. Qiao, Y. Sun, C. Liu, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 69, No. 1, 123-129 (2008).C. Qiao, S. Bi, Y. Sun, D. Song, H. Zhang, W. Zhou, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 70, No. 1, 136-143 (2008).C. Qiao, S. Bi, Y. Sun, D. Song, H. Zhang, W. Zhou, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 70, No. 1, 136-143 (2008).D. Xu, X. Wang, L. Ding, Carbohydr. Polym., 83, No. 3, 1257-1262 (2011).D. Xu, X. Wang, L. Ding, Carbohydr. Polym., 83, No. 3, 1257-1262 (2011).Y. Zhang, J. Pan, G. Zhang, X. Zhou, Sens. Actuat. B: Chem., 206, 630-639 (2015).Y. Zhang, J. Pan, G. Zhang, X. Zhou, Sens. Actuat. B: Chem., 206, 630-639 (2015).A. A. Ouameur, R. Marty, H. A. Tajmir-Riahi, Biopolymers, 77, No. 3, 129-136 (2005).A. A. Ouameur, R. Marty, H. A. Tajmir-Riahi, Biopolymers, 77, No. 3, 129-136 (2005).G. Zhang, X. Hu, J. Pan, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 78, No. 2, 687-694 (2011).G. Zhang, X. Hu, J. Pan, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 78, No. 2, 687-694 (2011).H. Ojha, B. M. Murari, S. Anand, M. I. Hassan, F. Ahmad, N. K. Chaudhury, Chem. Pharm. Bull., 57, No. 5, 481-486 (2009).H. Ojha, B. M. Murari, S. Anand, M. I. Hassan, F. Ahmad, N. K. Chaudhury, Chem. Pharm. Bull., 57, No. 5, 481-486 (2009).P. D. Ross, S. Subramanian, Biochemistry, 20, No. 11, 3096-3102 (1981).P. D. Ross, S. Subramanian, Biochemistry, 20, No. 11, 3096-3102 (1981).J. Min, X. M. Xia, Z. Dong, L. Yuan, L. X. Yu, C. Xing, J. Mol. Struct., 692, No. 1-3, 71-80 (2004).J. Min, X. M. Xia, Z. Dong, L. Yuan, L. X. Yu, C. Xing, J. Mol. Struct., 692, No. 1-3, 71-80 (2004).Y. Sun, S. Bi, D. Song, C. Qiao, D. Mu, H. Zhang, Sens. Actuat. B: Chem., 129, No. 2, 799-810 (2008).Y. Sun, S. Bi, D. Song, C. Qiao, D. Mu, H. Zhang, Sens. Actuat. B: Chem., 129, No. 2, 799-810 (2008).J. R. Lakowicz, G. Weber, Biochemistry, 12, No. 21, 4161-4170 (1973).J. R. Lakowicz, G. Weber, Biochemistry, 12, No. 21, 4161-4170 (1973).G. Chen, X. Huang, Z. Zheng, J. Xu, Z. Wang, Fluorescence Analytical Method, Science Press, Beijing (1990).G. Chen, X. Huang, Z. Zheng, J. Xu, Z. Wang, Fluorescence Analytical Method, Science Press, Beijing (1990).W. R. Ware, J. Phys. Chem., 66, No. 3, 455-458 (1962).W. R. Ware, J. Phys. Chem., 66, No. 3, 455-458 (1962).Y. Gao, J. Li, G. Huang, L. Yan, Z. Dong, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 141, 239-243 (2015).Y. Gao, J. Li, G. Huang, L. Yan, Z. Dong, Spectrochim. Acta, A: Mol. Biomol. Spectrosc., 141, 239-243 (2015).Y. Zhou, Y. Li, Coll. Surfaces A: Physicochem. Eng. Aspects, 233, No. 1-3, 129-135 (2004).Y. Zhou, Y. Li, Coll. Surfaces A: Physicochem. Eng. Aspects, 233, No. 1-3, 129-135 (2004).Z. Zhang, X. Dong, BioMetals, 22, No. 2, 283-288 (2009).Z. Zhang, X. Dong, BioMetals, 22, No. 2, 283-288 (2009).M. Guo, P. Yang, B. Yang, Z. Zhang, Chin. Sci. Bull., 49, No. 13, 1098-1103 (1996).M. Guo, P. Yang, B. Yang, Z. Zhang, Chin. Sci. Bull., 49, No. 13, 1098-1103 (1996).S. Tabassum, S. Parveen, F. Arjmand, Acta Biomater., 1, No. 6, 677-689 (2005).S. Tabassum, S. Parveen, F. Arjmand, Acta Biomater., 1, No. 6, 677-689 (2005).

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