zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-564Research Article******ОПТИМИЗАЦИЯ И ВАЛИДАЦИЯ СПЕКТРОФОТОМЕТРИЧЕСКИХ МЕТОДОВ АНАЛИЗА ГИДРОХЛОРИДА ДЕКСМЕДЕТОМИДИНА В ЧИСТОМ ВИДЕ И ЛЕКАРСТВЕННЫХ ФОРМАХOPTIMIZATION AND VALIDATION OF THE SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF DEXMEDETOMIDINE HYDROCHLORIDE IN PURE AND DOSAGE FORMSRoopaK. P.RoopaK. P.

Бангалор

Department of chemistry

Bangalore

roopakp@sapthagiri.edu.inBasavaiahK.BasavaiahK.

Майсур

Department of Studies in Chemistry, Manasagangothri Mysore

JayannaB. K.JayannaB. K.

Бангалор

Department of chemistry

Bangalore

Саптагири инженерный колледжИндияSapthagiri college of EngineeringIndiaМайсурский университетИндияUniversity of MysoreIndiaТехнологический институт Б.Н.М.ИндияB.N.M. Institute of technologyIndia201920032020864670(1)-670(8)Copyright © Roopa K.P., Basavaiah K., Jayanna B.K., 20202020Roopa K.P., Basavaiah K., Jayanna B.K.Roopa K.P., Basavaiah K., Jayanna B.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/564

Для анализа лекарственного препарата дексмедетомидина гидрохлорида в чистых и фармацевтических лекарственных формах разработаны четыре простых, точных, недорогих, чувствительных и применимых для различных целей УФ-вид-спектрофотометрических метода. Методы основаны на окислении лекарственного средства избытком N-бромсукцинимида (NBS) при комнатной температуре и оценке количества неиспользованного NBS с помощью амарантного красителя при λmax = 530 нм (метод A), сафранинового красителя при λmax = 530 нм (метод B), анилинового синего при λmax = 610 нм (метод C), родамина B при λmax = 560 нм (метод D). Регрессионный анализ зависимостей Ламберта-Бера подтвердил отличную корреляцию в диапазонах концентраций 2-9, 4-11, 670-2 2-10 и 1.2-3.5 мкг/мл для методов A, B, C и D соответственно. Рассчитаны кажущаяся молярная абсорбционная способность, чувствительность по Сэнделлу, пределы обнаружения и количественного определения.

Four simple, precise, low-cost, sensitive, and diversely applicable UV-Vis spectrophotometric methods have been developed for the assay of dexmedetomidine hydrochloride in pure and pharmaceutical dosage forms. The methods are based on the oxidation of the drug by N-bromosuccinimide (NBS) (excess) at room temperature and estimating the amount of unconsumed NBS by amaranth dye at λmax = 530 nm (method A), safranin dye at λmax = 530 nm (method B), aniline blue at λmax = 610 nm (method C), or rhodamine B at λmax = 560 nm (method D). Regression analysis of Beer-Lambert’s plots proves excellent correlation in the concentration ranges 2-9, 4-11, 2-10, and 1.2-3.5 μg/ml for methods A, B, C, and D, respectively. The apparent molar absorptivity, Sandell’s sensitivity, and detection and quantification limits are calculated. The proposed methods can be applied to drug formulation and recommended for routine analysis in quality control laboratories. Statistical comparison of the results with the reference method shows excellent agreement.

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The authors declare that there are no conflicts of interest present.