zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-625Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESСВЕРХЧУВСТВИТЕЛЬНОЕ ФЛУОРЕСЦЕНТНОЕ ОПРЕДЕЛЕНИЕ 6-ТИОГУАНИНА В БИОЛОГИЧЕСКИХ ОБРАЗЦАХ НА ОСНОВЕ ОПОСРЕДОВАННОГО НАНОЧАСТИЦАМИ СЕРЕБРА ВЫСВОБОЖДЕНИЯ ЗОНДА АКРИДИНОВОГО ОРАНЖЕВОГОULTRASENSITIVE FLUORESCENCE DETERMINATION OF 6-THIOGUANINE IN BIOLOGICAL SAMPLES BASED ON THE SILVER NANOPARTICLE-MEDIATED RELEASE OF ACRIDINE ORANGE PROBEVermaV. K.VermaV. K.

492010, Райпур

Department of Chemistry

492010, Raipur

TapadiaK.TapadiaK.

492010, Райпур

Department of Chemistry

492010, Raipur

ktapadia.chy@nitrr.ac.inMaharanaT.MaharanaT.

492010, Райпур

Department of Chemistry

492010, Raipur

SharmaA.SharmaA.

492010, Райпур

Department of Chemistry

492010, Raipur

Национальный технологический институт, ЧхаттисгархNational Institute of Technology, CG202008052020872345(1)-345(8)Copyright © Verma V.K., Tapadia K., Maharana T., Sharma A., 20202020Verma V.K., Tapadia K., Maharana T., Sharma A.Verma V.K., Tapadia K., Maharana T., Sharma A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/625

Представлен простой и чувствительный метод определения содержания 6-тиогуанина (6-TG). Метод основан на резонансном переносе энергии флуоресценции, при котором донором служит акридиновый оранжевый (АО), акцептором — стабилизированные цитратом наночастицы серебра (Ag-НЧ). Обнаружена нековалентная связь между поверхностями Ag-НЧ и АО, наблюдается эффект тушения флуоресценции. Спектры флуоресценции АО, регистрируемые после дальнейшего добавления 6-TG, свидетельствуют об агрегации Ag-НЧ. В идеальных условиях линейная зависимость имеет место в диапазоне концентраций 0.005—0.04 мкм, предел обнаружения 6-TG 5.3 нмоль. Метод дает возможность проведения экспресс-анализа содержания 6-TG в образцах сыворотки, крови и мочи человека.

This article reports a simple, convenient, and very sensitive method for the determination of 6-thioguanine (6-TG). The basic phenomenon in the proposed method is fluorescence resonance energy transfer, where acridine orange (AO) act as a donor and citrate-stabilized silver nanoparticles (AgNPs) as an acceptor. A noncovalent bond between the surfaces of AgNPs and AO was found, and the effect of fluorescence quenching was observed. The fluorescence spectra of AO recovered after further addition of 6-TG are responsible for the aggregation of AgNPs. Under ideal conditions, the linear relationship of 6-TG in the concentration range of 0.005 to 0.04 μM was displayed, and the limit of detection of 6-TG was obtained as 5.3 nM. Under ideal conditions, the linear relationship of 6-TG was displayed in the concentration range of 0.005 to 0.04 μM, and the limit of detection of 6-TG was obtained as 5.3 nM. The proposed method offers a rapid analysis to determine 6-TG in human serum, blood, and urine samples.

флуоресценциянаночастица серебра6-тиогуанинакридиновый оранжевыйfluorescencesilver nanoparticles6-thioguanineacridine orangeThe authors thank the CRF laboratory of IIT Kharagpur for HR-TEM analysis and National Institute of Technology, Raipur for providing necessary facilities.ReferencesT. P. Huynh, A. Wojnarowicz, M. Sosnowska, S. Srebnik, T. Benincori, F. Sannicolo, F. D’Souza, W. Kutner, Biosens. Bioelectron, 70, 153–160 (2015).T. P. Huynh, A. Wojnarowicz, M. Sosnowska, S. Srebnik, T. Benincori, F. Sannicolo, F. D’Souza, W. Kutner, Biosens. Bioelectron, 70, 153–160 (2015).A. A. Ensafi, H. Karimi-Maleh, J. Electroanal. Chem., 640, 75–83 (2010).A. A. Ensafi, H. Karimi-Maleh, J. Electroanal. Chem., 640, 75–83 (2010).Munshi, N. Pashna, M. Lubin, J. R. Bertino, Oncologist, 19, 760–765 (2014).Munshi, N. Pashna, M. Lubin, J. R. Bertino, Oncologist, 19, 760–765 (2014).H. Beitollahi, S. G. Ivari, M. T. Mahani, Mater. Sci. Eng. C, 69, 128–133 (2016).H. Beitollahi, S. G. Ivari, M. T. Mahani, Mater. Sci. Eng. C, 69, 128–133 (2016).Q. Gueranger, F. Li, M. Peacock, A. Larnicol-Fery, R. Brem, P. Macpherson, J. M. Egly, P. Karran, J. Invest. Dermatol., 134, 1408–1417 (2014).Q. Gueranger, F. Li, M. Peacock, A. Larnicol-Fery, R. Brem, P. Macpherson, J. M. Egly, P. Karran, J. Invest. Dermatol., 134, 1408–1417 (2014).M. T. Osterman, R. Kundu, G. R. Lichtenstein, J. D. Lewis, Gastroenterology, 130, 1047–1053 (2006).M. T. Osterman, R. Kundu, G. R. Lichtenstein, J. D. Lewis, Gastroenterology, 130, 1047–1053 (2006).R. Zakrzewski, J. Anal. Chem., 64, 1235–1241 (2009).R. Zakrzewski, J. Anal. Chem., 64, 1235–1241 (2009).B. B. Prasad, R. Singh, A. Kumar, Carbon, 102, 86–96 (2016).B. B. Prasad, R. Singh, A. Kumar, Carbon, 102, 86–96 (2016).U. Hindorf, M. Lindqvist, C. Peterson, P. Soderkvist, M. Strom, H. Hjortswang, Inflamm. Bowel Dis., 55, 1423–1431 (2006).U. Hindorf, M. Lindqvist, C. Peterson, P. Soderkvist, M. Strom, H. Hjortswang, Inflamm. Bowel Dis., 55, 1423–1431 (2006).G. Cangemi, A. Barabino, S. Barco, A. Parodi, S. Arrigo, G. Melioli, Int. J. Immunopathol. Pharmacol., 25, 435–444 (2012).G. Cangemi, A. Barabino, S. Barco, A. Parodi, S. Arrigo, G. Melioli, Int. J. Immunopathol. Pharmacol., 25, 435–444 (2012).H. Li, X. Chong, Y. Chen, L. Yang, L. Luo, B. Zhao, Y. Tian, Colloids Surf. A, 493, 52–58 (2016).H. Li, X. Chong, Y. Chen, L. Yang, L. Luo, B. Zhao, Y. Tian, Colloids Surf. A, 493, 52–58 (2016).Jacobsen, J. H. Schmiegelow, K. Nersting, J. Chromatogr. B, 881-882, 115–118 (2012).Jacobsen, J. H. Schmiegelow, K. Nersting, J. Chromatogr. B, 881-882, 115–118 (2012).S. A. Coultharda, P. Berry, S. McGarrity, A. Ansari, Christopher P. F. Redfern, J. Chromatogr. B, 1028, 175–180 (2016).S. A. Coultharda, P. Berry, S. McGarrity, A. Ansari, Christopher P. F. Redfern, J. Chromatogr. B, 1028, 175–180 (2016).N. Bi, M. Hu, H. Zhu, H. Qi, Y. Tian, Hanqi Zhang, Spectrochim. Acta A, 107, 4–30 (2013).N. Bi, M. Hu, H. Zhu, H. Qi, Y. Tian, Hanqi Zhang, Spectrochim. Acta A, 107, 4–30 (2013).X. Y. Deng, Y. Tang, L. H. Wu, L. J. Liu, X. Wang, Y. H. Chen, H. Q. Zhang, Y. Tian, Chin. J. Anal. Chem., 37, 79 (2009).X. Y. Deng, Y. Tang, L. H. Wu, L. J. Liu, X. Wang, Y. H. Chen, H. Q. Zhang, Y. Tian, Chin. J. Anal. Chem., 37, 79 (2009).P. D. Tzanavaras, D. G. Themelis, A. Economou, Anal. Chim. Acta, 505, 167–171 (2004).P. D. Tzanavaras, D. G. Themelis, A. Economou, Anal. Chim. Acta, 505, 167–171 (2004).W. Wang, S. F. Wang, F. Xie, Sens. Actuat. B, 120, 238–244 (2016).W. Wang, S. F. Wang, F. Xie, Sens. Actuat. B, 120, 238–244 (2016).M. Amjadi, L. Farzampour, J. Lumin., 29, 689 (2014).M. Amjadi, L. Farzampour, J. Lumin., 29, 689 (2014).E. Mozioglu, M. Akgoz, T. Kocagoz, C. Tamerler, Anal. Methods, 8, 4017–4021 (2016).E. Mozioglu, M. Akgoz, T. Kocagoz, C. Tamerler, Anal. Methods, 8, 4017–4021 (2016).Shi, Jingyu, F. Tian, J. Lyu, M. Yang, J. Mater. Chem. B, 35, 6989–7005 (2015).Shi, Jingyu, F. Tian, J. Lyu, M. Yang, J. Mater. Chem. B, 35, 6989–7005 (2015).J. N. Miller, Analyst, 130, 265–270 (2005).J. N. Miller, Analyst, 130, 265–270 (2005).P. Blaszkiewicz, M. Kotkowiak, A. Dudkowiak, J. Lumin., 183, 303–310 (2017).P. Blaszkiewicz, M. Kotkowiak, A. Dudkowiak, J. Lumin., 183, 303–310 (2017).Y. Chen, L. Chen, Y. Ou, L. Guo, F. Fu, Sens. Actuat. B: Chem., 233, 691–696 (2016).Y. Chen, L. Chen, Y. Ou, L. Guo, F. Fu, Sens. Actuat. B: Chem., 233, 691–696 (2016).Y. L. Xu, X. Y. Niu, H. J. Zhang, L. F. Xu, S. G. Zhao, H. L. Chen, X. G. Chen, J. Agric. Food Chem., 63, 1747–1755 (2015).Y. L. Xu, X. Y. Niu, H. J. Zhang, L. F. Xu, S. G. Zhao, H. L. Chen, X. G. Chen, J. Agric. Food Chem., 63, 1747–1755 (2015).F. Gao, Q. Ye, P. Cui, X. Chen, M. Li, L. Wang. Anal. Methods, 3, 1180–1185 (2011).F. Gao, Q. Ye, P. Cui, X. Chen, M. Li, L. Wang. Anal. Methods, 3, 1180–1185 (2011).K. H. Lee, S. J. Chen, J. Y. Jeng, Y. C. Cheng, J. T. Shiea, H. T. Chang, Colloid Interface Sci., 307, 340 (2007).K. H. Lee, S. J. Chen, J. Y. Jeng, Y. C. Cheng, J. T. Shiea, H. T. Chang, Colloid Interface Sci., 307, 340 (2007).P. Liu, Y. Zhou, M. Guo, S. Yang, Nanoscale, 10, 848–855 (2018).P. Liu, Y. Zhou, M. Guo, S. Yang, Nanoscale, 10, 848–855 (2018).N. Y. Chena, H. F. Lia, Z. F. Gaoa, F. Qub, N. B. Lia, H. Q. Luoa, Sens. Actuat. B: Chem., 193, 730–736 (2014).N. Y. Chena, H. F. Lia, Z. F. Gaoa, F. Qub, N. B. Lia, H. Q. Luoa, Sens. Actuat. B: Chem., 193, 730–736 (2014).W. Leesutthiphonchai, W. Dungchai, W. Siangproh, N. Ngamrojnavanich, O. Chailapakul, Talanta, 85, 870–876 (2011).W. Leesutthiphonchai, W. Dungchai, W. Siangproh, N. Ngamrojnavanich, O. Chailapakul, Talanta, 85, 870–876 (2011).U. P. Raghavendra, J. Thipperudrappa, M. Basanagouda, R. M. Melavanki, J. Lumin., 172, 139–146 (2016).U. P. Raghavendra, J. Thipperudrappa, M. Basanagouda, R. M. Melavanki, J. Lumin., 172, 139–146 (2016).L. Farzampour, M. Amjadi, J. Lumin., 155, 226–230 (2014).L. Farzampour, M. Amjadi, J. Lumin., 155, 226–230 (2014).S. Qadri, A. Ganoe, Y. Haik, J. Hazard. Mater., 169, 318–323 (2009).S. Qadri, A. Ganoe, Y. Haik, J. Hazard. Mater., 169, 318–323 (2009).J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Kluwer, New York (1999).J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Kluwer, New York (1999).G. L. Wang, W. M. Dong, X. Y. Zhu, W. J. Zhang, C. Wang, H. J. Jiao, Analyst, 136, 5256–5260 (2011).G. L. Wang, W. M. Dong, X. Y. Zhu, W. J. Zhang, C. Wang, H. J. Jiao, Analyst, 136, 5256–5260 (2011).H. C. Dai, Y. Shi, Y. L. Wang, Y. J. Sun, J. T. Hu, P. J. Ni, Sens. Actuat. B: Chem., 202, 201–208 (2014).H. C. Dai, Y. Shi, Y. L. Wang, Y. J. Sun, J. T. Hu, P. J. Ni, Sens. Actuat. B: Chem., 202, 201–208 (2014).A. M. E. Badawy, T. P. Luxton, R. G. Silva, K. G. Scheckel, M. T. Suidan, T. M. Tolaymat, Environ. Sci. Technol., 44, 1260–1266 (2010).A. M. E. Badawy, T. P. Luxton, R. G. Silva, K. G. Scheckel, M. T. Suidan, T. M. Tolaymat, Environ. Sci. Technol., 44, 1260–1266 (2010).

The authors declare that there are no conflicts of interest present.