zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-709Research ArticleСтатьиФЛУОРЕСЦЕНТНОЕ ОБНАРУЖЕНИЕ ГЛУТАТИОНА С ИСПОЛЬЗОВАНИЕМ НАНОСИСТЕМЫ N-ЛЕГИРОВАННЫЕ ГРАФЕНОВЫЕ КВАНТОВЫЕ ТОЧКИ–MnO2FLUORESCENCE DETECTION OF GLUTATHIONE USING N-DOPED GRAPHENE QUANTUM DOTS−MnO2 NANOARCHITECTURELiZh.-M.LiZhi-Mei

Наньчан 330031

Nanchang 330031

PiT.PiTing

Наньчан 330031

Nanchang 330031

ShengY.-P.ShengYa-Ping

Хэфэй 230026

Hefei 230026

ZhengX.-J.ZhengXiang-Juan

Наньчан, 330031

Nanchang 330031

zhengxiangjuan@ncu.edu.cnХимический колледж Наньчанского университетаCollege of Chemistry, Nanchang UniversityШкола химии и материаловедения Китайского университета науки и технологийSchool of Chemistry and Materials Science, University of Science and Technology of ChinaХимический колледж Наньчанского университета; Главная лаборатория многоуровневых междисциплинарных исследований ЦзянсиCollege of Chemistry, Nanchang University; Jiangxi Key Laboratory for Multiscale Interdisciplinary Study202002112020875849(1)-849(8)Copyright © Li Z., Pi T., Sheng Y., Zheng X., 20202020Li Z., Pi T., Sheng Y., Zheng X.Li Z., Pi T., Sheng Y., Zheng X.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/709

Предложен флуоресцентный метод определения уровней глутатиона (GSH) в сыворотке крови человека с использованием нанокомпозита квантовых точек графена (NGQD) и MnO2, легированного N. Нанолисты MnO2 на поверхности NGQD служат в качестве тушителя. Флуоресценция NGQD может восстанавливаться за счет добавления GSH, который восстанавливает MnO2 до Mn2+ , таким образом можно контролировать уровень GSH. Платформа MnO2 обеспечивает минимальный фон и высокую чувствительность для обнаружения GSH в предлагаемой схеме. Отслежены соответствующие процессы включения-выключения флуоресценции и исследован механизм восприятия.

We build a novel fluorescence resonance energy transfer (FRET) method based on N-doped graphene quantum dots (NGQDs)-MnO2 nanocomposite for rapid, sensitive detection of glutathione (GSH) levels in human serum. In this strategy, MnO2 nanosheets on the NGQDs surface serve as a quencher. NGQDs fluorescence can make a recovery by the addition of GSH, which can reduce MnO2 to Mn2+, and thus the GSH can be monitored. The MnO2 platform affords minimal background and high sensitivity for detecting GSH in this proposed scheme. Meanwhile, relevant fluorescence on-off-on processes were monitored, and the sensing mechanism was explored.

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The authors declare that there are no conflicts of interest present.