zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-714Research ArticleСтатьиОПРЕДЕЛЕНИЕ ЛОРАЗЕПАМА В ЛЕКАРСТВЕННЫХ ПРЕПАРАТАХ И БИОЛОГИЧЕСКИХ ЖИДКОСТЯХ С ИСПОЛЬЗОВАНИЕМ ЧУВСТВИТЕЛЬНОЙ СПЕКТРОФОТОМЕТРИИ И МЕТОДОЛОГИИ ПОВЕРХНОСТИ ОТКЛИКАDETERMINATION OF LORAZEPAM IN DRUG FORMULATION AND BIO FLUIDS USING A SPECTROPHOTOMETRIC METHOD AND RESPONSE SURFACE METHODOLOGYGhorbanpoorS.GhorbanpoorSara

Йезд

Yazd

ShishehborM. R.ShishehborM. Reza

Йезд

Yazd

shishehbor47@iauyazd.ac.irSheibaniA.SheibaniAli

Йезд

Yazd

SafaeiM.SafaeiMohadeseh

Йезд

Yazd

NazariA.NazariAli

Йезд

Yazd

Исламский университет АзадDepartment of Chemistry, Yazd Branch, Islamic Azad University202002112020875854(1)-854(11)Copyright © Ghorbanpoor S., Shishehbor M.R., Sheibani A., Safaei M., Nazari A., 20202020Ghorbanpoor S., Shishehbor M.R., Sheibani A., Safaei M., Nazari A.Ghorbanpoor S., Shishehbor M.R., Sheibani A., Safaei M., Nazari A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/714

Разработан простой, чувствительный и селективный спектрофотометрический метод определения лоразепама в фармацевтических препаратах и биологических образцах. Процедура основана на каталитическом влиянии лоразепама на реакционную систему Янус Грин-бромат. Изменение оптической плотности зарегистрировано с помощью спектрофотометра на λ = 618 нм. Для получения максимальной чувствительности концентрация реагентов температура и время оптимизировались последовательно по одному параметру. При оптимальных условиях эксперимента калибровочная кривая в диапазоне концентраций 0.3–19.5 мкг/мл лоразепама состоит из двух линейных участков. Относительные стандартные отклонения (n = 5) для концентраций лоразепама 1.0, 5.0 и 15.0 мкмоль/л составили 1.09, 1.03 и 0.97 % соответственно. Предел обнаружения лоразепама 0,08 мкг/мл. Экспериментальная проверка в оптимальных условиях в соответствии со схемой спецификаций требований показала хорошие результаты. Разработанный метод успешно применен для определения лоразепама в реальных образцах. Результаты хорошо согласуются с полученными методами высокоэффективной жидкостной хроматографии.

A novel, simple, sensitive, and selective kinetic spectrophotometric method has been developed for the determination of lorazepam in pharmaceutical and bioloical samples. The procedure is based on the catalytic effect of lorazepam on the Janus Green-bromate reaction system. The change in absorbance was followed spectrophotometrically at 618 nm. To obtain the maximum sensitivity, the reagents concentration, temperature, and time were optimized by one at the time method. Under optimum experimental conditions, the calibration curve was linear over the range 0.3–19.5 μg/mL of lorazepam, including two linear segments. The relative standard deviations (n = 5) for 1.0, 5.0, and 15.0 μmol/L of lorazepam were 1.09, 1.03, and 0.97%, respectively. The limit of detection was 0.08 μg/mL of lorazepam. An experimental check under these optimal conditions confirmed good agreement in the RSM results. The developed method was successfully applied for the determination of lorazepam in real samples, and the obtained results are in a good agreement with those using HPLC.

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The authors declare that there are no conflicts of interest present.