zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-772Research ArticleСтатьиВЫДЕЛЕНИЕ И ИДЕНТИФИКАЦИЯ 9-цис-АСТАКСАНТИНА С ПОМОЩЬЮ ВЫСОКОЖИДКОСТНОЙ ЭЛЕКТРОННОЙ ХРОМАТОГРАФИИ, ИК-ФУРЬЕ И ЯМР-СПЕКТРОВISOLATION AND IDENTIFICATION OF 9-cis ASTAXANTHIN BY HPLC, FT-IR, AND NMR SPECTRAWangY.WangY.

Нинбо, 315211

Ningbo 315211

WuY.WuY.

Синьчанг, 312500

High-Tech Industry Zone, XinChang 312500

WangT.WangT.

Нинбо, 315211

Ningbo 315211

QiuD.QiuD.

Нинбо, 315211;

Синьчанг, 312500

Ningbo 315211;

High-Tech Industry Zone, XinChang 312500

wangyajuan@nbut.edu.cnШкола материалов и химической инженерии Технологического университета НинбоSchool of Materials and Chemical Engineering at Ningbo University of TechnologyZhejiang NVB Co. Ltd.Zhejiang NVB Co. Ltd.Школа материалов и химической инженерии Технологического университета Нинбо; Zhejiang NVB Co. Ltd.School of Materials and Chemical Engineering at Ningbo University of Technology; Zhejiang NVB Co. Ltd.202129012021881109118Copyright © Wang Y., Wu Y., Wang T., Qiu D., 20212021Wang Y., Wu Y., Wang T., Qiu D.Wang Y., Wu Y., Wang T., Qiu D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/772

Выполнен детальный анализ изомеров астаксантина. Разработан метод быстрой хроматографии на открытой колонке для разделения геометрических изомеров астаксантина на уровне граммов. Хроматографическое разделение проведено с использованием двух силикагелевых колонок с дихлорметаном/н-гексаном/диэтиловым эфиром (2:1:1) в качестве системы элюирования. Выделенные изомеры идентифицированы как полностью транс-, 9-цис-, 13-цис- и 15-цис-астаксантин, согласно ранее опубликованным данным анализа C30 высокожидкостной электронной хроматографии. Тонкая структура единственного изомера 9-цис-астаксантина, а также полностью трансастаксантина определена с помощью ИК-Фурье-спектроскопии, одномерной 1H и 13C ЯМР-спектроскопии и двумерной гомоядерной корреляционной и гетероядерной корреляционной ЯМР-спектроскопии.

This study aims to perform a detailed qualitative analysis of astaxanthin isomers. A rapid, open column chromatography method was developed to separate astaxanthin geometrical isomers in gram-scale levels. Chromatographic separation was performed using two silica gel columns with dichloromethane/nhexane/diethyl ether (2:1:1, v/v/v) as elution system. The isolated isomers were identified as all-trans, 9-cis, 13-cis, and 15-cis astaxanthin, according to previously reported C30 high-performance liquid chromatography (HPLC) analysis data. Further, the fine structure of the single 9-cis astaxanthin isomer, as well as all-trans astaxanthin, was determined successfully for the first time by Fourier-transform infrared spectroscopy (FT-IR), one-dimensional 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, and twodimensional homonuclear correlation and heteronuclear correlation NMR spectroscopy.

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The authors declare that there are no conflicts of interest present.