zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-845Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESОптическое нанобиосенсирование стибоглюконата в плазме крови и урине человека с использованием флуоресцентных углеродных наноточек, синтезированных экологичным “зеленым” методомOptical Nanobiosensing of Stibogluconate in Plasma and Urine Using Green Synthesized Fluorescent Carbon NanodotsTalaatW.TalaatW.

Даманхур, 25111

Damanhour 25111

asmz68@sci.dmu.edu.egHassanA. F.HassanA. F.

Даманхур, 25111

Damanhour 25111

asmz68@sci.dmu.edu.egУниверситет Даман-ХурDamanhour University202129052021882333(1)-333(13)Copyright © Talaat W., Hassan A.F., 20212021Talaat W., Hassan A.F.Talaat W., Hassan A.F.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/845

Из кожуры чеснока синтезированы флуоресцентные углеродные наноточки в качестве оптического нанобиозонда для антилейшманиотического препарата стибоглюконата. Методы характеризации синтезированных углеродных наноточек подтвердили их термостойкость, наноразмерностъ в диапазоне 2-14 нм, аморфную природу и присутствие функциональных групп C-O. Разработан основанный на флуоресцентной природе углеродных наноточек оптический сенсор для нанобиозондирования лекарственных препаратов в таблетированной форме и биологических жидкостях человека. Препарат переваривали с высвобождением пятивалентного катиона сурьмы, который тушит флуоресценцию углеродных наноточек. Экстракция осуществлялась путем образования ионной пары с тринониламином. Анализ стибоглюконата оптическим методом осуществлен in vivo в реальной плазме человека. Характеристика обнаружения стибоглюконата линейная в пределах 0.01-0.10 мкг/мл при воспроизводимости 99.25±1.86 и 99.624±1.33 c добавленными плазмой крови и уриной.

Fluorescent carbon nanodots were synthetized from garlic peels as optical nanobiosensors for an anti-leishmaniotic stibogluconate drug. The characterization techniques for the synthetized carbon nanodots confirmed its thermal stability, nanosize in the range 2—14 nm, amorphous nature, and the presence of C—O functional groups. The optical sensor, based on the fluorescent nature of carbon nanodots, was developed for the drug nanobiosensor in dosage form and in human biological fluids. The drug was digested to release a pentavalent antimony cation that quenches the fluorescence intensity of carbon nanodots. The extraction method was performed through ion pairing with trinonylamine. The optical sensing was extended to the in vivo analysis of stibogluconate in real human plasma. The sensing of stibogluconate was found to be linear over the range 0.01-0.10 µg/mL with percentage recoveries of 99.25±1.86, and 99.624±1.33 in vials with spiked plasma and in spiked urine.

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The authors declare that there are no conflicts of interest present.