zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-948Research ArticleСтатьиИзучение кристаллической структуры, взаимодействия ДНК/белок и цитотоксичности мононуклеарного комплекса цинка(II) с ниациномNovel mononuclear zinc(II) complex with niacin: crystal structure, DNA/protein interaction, and cytotoxicity studiesZhaoC.ZhaoC.

Яньчэн, Цзянсу, 224051

Yancheng, Jiangsu, 224051

HuangL.HuangL.

Яньчэн, Цзянсу, 224005

Yancheng, Jiangsu, 224005

WangQ.WangQ.

Яньчэн, Цзянсу, 224005

Yancheng, Jiangsu, 224051

wangqm@yctu.edu.cnФармацевтическая школа Яньчэнского педагогического университетаSchool of Pharmacy, Yancheng Teachers’ UniversityНародная больница № 1 города ЯньчэнDepartment of Thoracic Surgery, Yancheng City No.1 People’s Hospital202129112021886972972Copyright © Zhao C., Huang L., Wang Q., 20212021Zhao C., Huang L., Wang Q.Zhao C., Huang L., Wang Q.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/948

Синтезирован новый одноядерный комплекс цинка(II) Zn(ниацин)2(H2O)4 (1). Взаимодействие комплекса 1 с ДНК тимуса теленка исследовано с помощью УФ-видимой и флуоресцентной спектрометрии. Установлена константа связывания Kapp = 4.6´105 М–1. Показано, что механизм тушения флуоресценции бычьего сывороточного альбумина в присутствии комплекса 1 может быть статическим гашением с одним местом связывания. Исследована противораковая активность комплекса на трех линиях опухолевых клеток человека (HT29, HepG2 и MCF-7). Значения IC50 комплекса 1 для HT29, HepG2, MCF-7 составляют 114.1±8, 146.7±14 и 319.2±13 мкМ соответственно. 

A new mononuclear zinc(II) complex Zn(niacin)2(H2O)4 (1) is synthesized. The interaction of 1 with calf thymus-DNA is investigated by UV-Vis and fluorescence spectrometry, and the apparent binding constant (Kapp) value is established as 4.6×105 M–1 . It is found that the quenching mechanism between bovine serum albumin and 1 might be a static quenching procedure with one binding site. Further, the anticancer activity of the complex on three human tumor cells lines (HT29, HepG2, and MCF-7) is studied. The IC50 values of 1 on HT29, HepG2, MCF-7 are 114.1±8, 146.7±14, and 319.2±13 µM, respectively. 

комплексы цинка(II)кристаллическая структурасвязывание ДНКсвязывание с белкамипротивораковая активностькруговой дихроизмzinc(II) complexescrystal structureDNA bindingprotein bindinganticancer activitycircular dichroismReferencesK. B. Garbutcheon-Singh, M. P. Grant, B. W Harper, A. M. Krause-Heuer, M. Manohar, N. Orkey, J. R. Aldrich-Wright, Curr. Top. Med. Chem., 11, 521–542 (2011).K. B. Garbutcheon-Singh, M. P. Grant, B. W Harper, A. M. Krause-Heuer, M. Manohar, N. Orkey, J. R. Aldrich-Wright, Curr. Top. Med. Chem., 11, 521–542 (2011).G. Y. Li, R. L. Guan, L. N. Ji, H. Chao, Coord. Chem. Rev., 281, 100–113 (2014).G. Y. Li, R. L. Guan, L. N. Ji, H. Chao, Coord. Chem. Rev., 281, 100–113 (2014).A. C. Komor, J. K. Barton, Chem. Commun., 49, 3617–3630 (2013).A. C. Komor, J. K. Barton, Chem. Commun., 49, 3617–3630 (2013).R. Senthil Kumar, S. Arunachalam, Polyhedron, 26, 3255–3262 (2007).R. Senthil Kumar, S. Arunachalam, Polyhedron, 26, 3255–3262 (2007).F. Xue, C. Z. Xie, Y. W. Zhang, Z. Qiao, X. Qiao, J. Y. Xu, S. Y. Yan, J. Inorg. Biochem., 115, 78–86 (2012).F. Xue, C. Z. Xie, Y. W. Zhang, Z. Qiao, X. Qiao, J. Y. Xu, S. Y. Yan, J. Inorg. Biochem., 115, 78–86 (2012).X. F. Zhao, Y. Ouyang, Y. Z. Liu, Q. J. Su, H. Tian, C. Z. Xie, J. Y. Xu. New J. Chem., 38, 955–965 (2011).X. F. Zhao, Y. Ouyang, Y. Z. Liu, Q. J. Su, H. Tian, C. Z. Xie, J. Y. Xu. New J. Chem., 38, 955–965 (2011).W. J. Lian, X. T. Wang, C. Z. Xie, H. Tian, X. Q. Song, H. T. Pan, X. Qiao, J. Y. Xu, Dalton Trans., 45, 9073–9087 (2016).W. J. Lian, X. T. Wang, C. Z. Xie, H. Tian, X. Q. Song, H. T. Pan, X. Qiao, J. Y. Xu, Dalton Trans., 45, 9073–9087 (2016).C. Y. Gao, Z. Y. Ma, Y. P. Zhang, S. T. Li, W. Gu, X. Liu, J. L. Tian, J. Y. Xu, J. Z. Zhao, S. P. Yan, RSC Adv., 5, 30768–30779 (2015).C. Y. Gao, Z. Y. Ma, Y. P. Zhang, S. T. Li, W. Gu, X. Liu, J. L. Tian, J. Y. Xu, J. Z. Zhao, S. P. Yan, RSC Adv., 5, 30768–30779 (2015).K. D. Mjos, C. Orvig, Chem. Rev., 114, 4540–4563 (2014).K. D. Mjos, C. Orvig, Chem. Rev., 114, 4540–4563 (2014).G. Barone, A. Terenzi, A. Lauria, A. M. Almerico, J. M. Leal, N. Busto, B. García, Coord. Chem. Rev., 257, 2848–2862 (2013).G. Barone, A. Terenzi, A. Lauria, A. M. Almerico, J. M. Leal, N. Busto, B. García, Coord. Chem. Rev., 257, 2848–2862 (2013).Q. Jiang, N. Xiao, P. Shi, Y. Zhu, Z. Guo, Coord. Chem. Rev., 251, 1951–1972 (2007).Q. Jiang, N. Xiao, P. Shi, Y. Zhu, Z. Guo, Coord. Chem. Rev., 251, 1951–1972 (2007).J. J. Wilson, S. J. Lippard, Chem. Rev., 114, 4470–4495 (2014).J. J. Wilson, S. J. Lippard, Chem. Rev., 114, 4470–4495 (2014).N. Muhammad, Z. Guo, Curr. Opin. Chem. Biol., 19, 144–153 (2014).N. Muhammad, Z. Guo, Curr. Opin. Chem. Biol., 19, 144–153 (2014).A. M. Florea, D. Busselberg, Cancers, 3, 1351–1371 (2011).A. M. Florea, D. Busselberg, Cancers, 3, 1351–1371 (2011).Z. Y. Ma, Z. Qiao, D. B. Wang, X. Hou, X. Qiao, C. Z. Xie, Z. Y. Qiang, J. Y. Xu, Appl. Organomet. Chem., 31, No. 7, e3651 (2017).Z. Y. Ma, Z. Qiao, D. B. Wang, X. Hou, X. Qiao, C. Z. Xie, Z. Y. Qiang, J. Y. Xu, Appl. Organomet. Chem., 31, No. 7, e3651 (2017).X. D. Lin, Y. H. Liu, C. Z. Xie, W. G. Bao, J. Shen, J H. Xu, RSC Adv., 7, No. 42, 26478–26486 (2017).X. D. Lin, Y. H. Liu, C. Z. Xie, W. G. Bao, J. Shen, J H. Xu, RSC Adv., 7, No. 42, 26478–26486 (2017).Q. M. Wang, L. Yang, J. H. Wu, H. Wang, J. L. Song, X. H. Tang, Biometals, 30, No. 1, 17–26 (2017).Q. M. Wang, L. Yang, J. H. Wu, H. Wang, J. L. Song, X. H. Tang, Biometals, 30, No. 1, 17–26 (2017).C. H. Leung, H. Z. He, L. J. Liu, M. Wang, D. S. H. Chan, D. L. Ma, Coord. Chem. Rev., 257, 3139–3151 (2013).C. H. Leung, H. Z. He, L. J. Liu, M. Wang, D. S. H. Chan, D. L. Ma, Coord. Chem. Rev., 257, 3139–3151 (2013).H. K. Liu, P. J. Sadler, Acc. Chem. Res., 44, 349–359 (2011).H. K. Liu, P. J. Sadler, Acc. Chem. Res., 44, 349–359 (2011).Y. Aiba, J. Sumaoka, M. Komiyama, Chem. Soc. Rev., 40, 5657–5668 (2011).Y. Aiba, J. Sumaoka, M. Komiyama, Chem. Soc. Rev., 40, 5657–5668 (2011).O. K. Abou-Zied, Curr. Pharm. Des., 21, 1800–1816 (2015).O. K. Abou-Zied, Curr. Pharm. Des., 21, 1800–1816 (2015).R. E. Olson, D. D. Christ, Annu. Rep. Med. Chem., 31, 327–336 (1996).R. E. Olson, D. D. Christ, Annu. Rep. Med. Chem., 31, 327–336 (1996).J. Maiti, S. Biswas, A. Chaudhuri, S. Chakraborty, S. Chakraborty, R. Das, Spectrochim. Acta A, 175, 191–199 (2017).J. Maiti, S. Biswas, A. Chaudhuri, S. Chakraborty, S. Chakraborty, R. Das, Spectrochim. Acta A, 175, 191–199 (2017).S. Ghosh, S. Chakrabarty, D. Bhowmik, G. S. Kumar, N. Chattopadhyay, J. Phys. Chem. B, 119, 2090–2102 (2015).S. Ghosh, S. Chakrabarty, D. Bhowmik, G. S. Kumar, N. Chattopadhyay, J. Phys. Chem. B, 119, 2090–2102 (2015).V. Kolenko, E. Teper, A. Kutikov, R. Uzzo, Nat. Rev. Urol., 10, 219–226 (2013).V. Kolenko, E. Teper, A. Kutikov, R. Uzzo, Nat. Rev. Urol., 10, 219–226 (2013).Q. M. Wang, H. Mao, W. L. Wang, H. M. Zhu, L.H. Dai, Y. L. Chen, Xinhui Tang, Biometals, 30, No. 4, 575–587 (2017).Q. M. Wang, H. Mao, W. L. Wang, H. M. Zhu, L.H. Dai, Y. L. Chen, Xinhui Tang, Biometals, 30, No. 4, 575–587 (2017).G. M. Sheldrick. SADABS, University of Göttingen, Germany (2000).G. M. Sheldrick. SADABS, University of Göttingen, Germany (2000).G. M. Sheldrick. SHELXS 97 and SHELXL 97, University of Göttingen, Germany (1997).G. M. Sheldrick. SHELXS 97 and SHELXL 97, University of Göttingen, Germany (1997).Bruker, SMART (Version 5.0) and SAINT (Version 6.02), Bruker AXS Inc., Madison, Wisconsin, USA (2000).Bruker, SMART (Version 5.0) and SAINT (Version 6.02), Bruker AXS Inc., Madison, Wisconsin, USA (2000).P. Grzegorz, Arch Biochem. Biophys., 453, 54–62 (1978).P. Grzegorz, Arch Biochem. Biophys., 453, 54–62 (1978).J. Carmichael, W. G. DeGraff, A. F. Gazdar, J. D. Minna, J. B. Mitchell, Cancer Res., 47, 936–942 (1978).J. Carmichael, W. G. DeGraff, A. F. Gazdar, J. D. Minna, J. B. Mitchell, Cancer Res., 47, 936–942 (1978).H. Han, L. P. Lu, Q. M. Wang, M. L. Zhu, C. X. Yuan, S. Xing, X. Q. Fu, Dalton Trans., 41, 111116–111124 (2012).H. Han, L. P. Lu, Q. M. Wang, M. L. Zhu, C. X. Yuan, S. Xing, X. Q. Fu, Dalton Trans., 41, 111116–111124 (2012).Q. M. Wang, M. L. Zhu, L. P. Lu, C. X. Yuan, S. Xing, X. Q. Fu. Dalton Trans., 2011, 40, 12926–12934.Q. M. Wang, M. L. Zhu, L. P. Lu, C. X. Yuan, S. Xing, X. Q. Fu. Dalton Trans., 2011, 40, 12926–12934.L. E. Ta, L. Laura Espeset, J. Jewel Podratz, A. J. Windebank, Neurotoxicology, 27, 992–1002 (2006).L. E. Ta, L. Laura Espeset, J. Jewel Podratz, A. J. Windebank, Neurotoxicology, 27, 992–1002 (2006).S. Dhar, D. Senapati, P. K. Das, P. Chatopadhyay, M. Nethaji, A. R. Chakravarty, J. Am. Chem. Soc., 125, 12118–12124 (2003).S. Dhar, D. Senapati, P. K. Das, P. Chatopadhyay, M. Nethaji, A. R. Chakravarty, J. Am. Chem. Soc., 125, 12118–12124 (2003).E. R. Jamieson, S. J. Lippard, Chem. Rev., 99, 2467–2498 (1999).E. R. Jamieson, S. J. Lippard, Chem. Rev., 99, 2467–2498 (1999).S. S. Bhat, A. A. Kumbhar, H. Heptullah, A. A. Khan, V. V. Gobre, S. P. Gejji, V. G. Puranik, Inorg. Chem., 50, 545–558 (2011).S. S. Bhat, A. A. Kumbhar, H. Heptullah, A. A. Khan, V. V. Gobre, S. P. Gejji, V. G. Puranik, Inorg. Chem., 50, 545–558 (2011).P. Sevilla, J. M. Rivas, F. GarcÍa-Blanco, J. V. García-Ramos, S. Sánchez-Cortés, Biochim. Biophys. Acta, 1774, 1359–1369 (2007).P. Sevilla, J. M. Rivas, F. GarcÍa-Blanco, J. V. García-Ramos, S. Sánchez-Cortés, Biochim. Biophys. Acta, 1774, 1359–1369 (2007).J. B. LePecq, C. C. Paoletti, J. Mol. Biol., 27, 87–106 (1976).J. B. LePecq, C. C. Paoletti, J. Mol. Biol., 27, 87–106 (1976).A. Gohel, M. B. McCarthy, G. Gronowicz, Endocrinology, 140, 5339–5347 (1999).A. Gohel, M. B. McCarthy, G. Gronowicz, Endocrinology, 140, 5339–5347 (1999).G. Y. Li, K. J. Du, J. Q. Wang, J. W. Liang, J. F. Kou, X. J. Hou, L. N. Ji, H. Chao, J. Inorg. Biochem., 119, 43–53 (2013).G. Y. Li, K. J. Du, J. Q. Wang, J. W. Liang, J. F. Kou, X. J. Hou, L. N. Ji, H. Chao, J. Inorg. Biochem., 119, 43–53 (2013).A. T. M. Marcelis, J. H. J. Den Hartog, G. A. Van der Marel, G. Wille, J. Reedijk, Eur. J. Biochem., 135, 343–349 (1983).A. T. M. Marcelis, J. H. J. Den Hartog, G. A. Van der Marel, G. Wille, J. Reedijk, Eur. J. Biochem., 135, 343–349 (1983).R. C. Srivastava, J. Froehlich, G. L. Eichhorn, Biochimie, 60, 879–891 (1978).R. C. Srivastava, J. Froehlich, G. L. Eichhorn, Biochimie, 60, 879–891 (1978).J. P. Macquet, J. L. Butour, Eur. J. Biochem., 83, 375–387 (1978).J. P. Macquet, J. L. Butour, Eur. J. Biochem., 83, 375–387 (1978).L. Cheng, C. Bulmer, A. Margaritis, Curr. Drug Deliv., 12, 351–357 (2009).L. Cheng, C. Bulmer, A. Margaritis, Curr. Drug Deliv., 12, 351–357 (2009).X. L. Shi, X. W. Li, M. Y. Gui, H. Y. Zhou, R. J. Yang, H. Q. Zhang, J. R. Jin, J. Lumin., 130, 637–644 (2009).X. L. Shi, X. W. Li, M. Y. Gui, H. Y. Zhou, R. J. Yang, H. Q. Zhang, J. R. Jin, J. Lumin., 130, 637–644 (2009).X. J. Guo, L. Zhang, X. D. Sun, X. W. Han, C. Guo, P. L. Kang, J. Mol. Struct., 928, 114–120 (2009).X. J. Guo, L. Zhang, X. D. Sun, X. W. Han, C. Guo, P. L. Kang, J. Mol. Struct., 928, 114–120 (2009).P. D. Ross, S. Subramanian, Biochemistry, 20, 3096–3102 (1978).P. D. Ross, S. Subramanian, Biochemistry, 20, 3096–3102 (1978).

The authors declare that there are no conflicts of interest present.