zhpsЖурнал прикладной спектроскопииZhurnal Prikladnoii Spektroskopii0514-7506B. I. Stepanov Institute of Physics of the National Academy of Scienceszhps-984Research ArticleАННОТАЦИИ АНГЛОЯЗЫЧНЫХ СТАТЕЙABSTRACTS ENGLISH-LANGUAGE ARTICLESУФ-спектрофотометрический метод определения хризина и его растворимостиDevelopment and Validation of UV Spectrophotometric Method for Determination of Chrysin and Its Solubility StudiesBansalA.BansalA.

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SrivastavaN.SrivastavaN.

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NagpalK.NagpalK.

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knchaswal@amity.eduФармацевтический институт Университета Уттар-ПрадешаИндияAmity Institute of Pharmacy, Amity University Uttar PradeshIndia202204022022891134142Copyright © Bansal A., Srivastava N., Nagpal K., 20222022Bansal A., Srivastava N., Nagpal K.Bansal A., Srivastava N., Nagpal K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zhps.ejournal.by/jour/article/view/984

Разработан УФ-спектрофотометрический метод количественной оценки хризина. Изучено влияние природы растворителя на растворимость хризина. Исходный и разбавленный растворы сканировали с использованием УФ-видимого спектрофотометра для получения λmax. Для построения калибровочной кривой регистрировали оптическую плотность образцов и с использованием программного обеспечения MS Excel и Sigma stat устанавливали регрессионную зависимость. Разработанный метод дополнительно проверен на линейность, точность, пределы обнаружения и количественной оценки, надежность. Растворимость хризина определяли при 35 °C в различных маслах, растворителях и сорастворителях. Обнаружено, что λmax хризина в метаноле составляет 367 нм. Калибровочная кривая препарата линейна в диапазоне 2–10 мкг/мл с коэффициентом корреляции 0.991. Точность метода проверена для концентрации аналита 80, 100 и 120 % при степени извлечения 97–99.5 %. Прецизионные исследования проводились с учетом суточных и межсуточных вариаций. Надежность предложенного метода изучалась с привлечением двух аналитиков. Обнаружено, что растворимость хризина максимальна в метаноле (216.80±0.0097 мкг/мл) в сравнении с используемыми маслами, растворителями и сорастворителями. Проведенные эксперименты показывают точность и воспроизводимoсть разработанного метода. Вязкость растворителя и возможность образования водородных связей – решающие факторы, влияющие на растворимость хризина.

The present work is based on the method development and validation of the UV-spectrophotometric method for the quantitative estimation of chrysin. We studied how the nature of the solvent affects chrysin solubility. The stock and diluted solutions were scanned using a UV visible spectrophotometer to obtain the λmax. The absorbance of the samples was recorded to obtain a calibration curve, which was followed by regression using MS Excel and Sigma stat software. The method developed by this process was further validated using parameters such as linearity, precision, limit of detection, limit of quantification, accuracy, and ruggedness. The solubility of chrysin was checked at 35oC in different oils, solvents, and co-solvents. The λmax of chrysin in methanol was found to be 367 nm. The calibration curve of the drug follows linearity (2–10 μg/mL) with a correlation coefficient of 0.991. At three different levels, i.e., 80, 100, and 120%, the method’s accuracy was checked utilizing the percent recovery (97–99.5%). The precision studies were carried out in terms of intraday and interday variations. The ruggedness of the proposed method was studied by taking two. The solubility of chrysin was found to be the maximum in methanol (216.80±0.0097 μg/mL) among the oils, solvents, and co-solvents used. Thus, based on the experiments done, the developed method was observed to be accurate, precise, and reproducible. The viscosity of the solvent and the possibility of hydrogen bonding are two crucial factors that affected the solubility of chrysin in the solvents.

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The authors declare that there are no conflicts of interest present.