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Rapid Spectrophotometric Method for Diazepam Quantification in Pharmaceutical Formulations Using Ion Pair Formation with Methyl Orange and Bromophenol Blue
Abstract
An extractive visible spectrophotometric procedure has been developed for diazepam (DZ) determination in pure and pharmaceutical forms using methyl orange (MO) and bromophenol blue (BPB) dyes. The proposed method was based on the formation of an ion-pair colored complex between diazepam and indicators via extracting them into chloroform whereby absorbance measured at 411 and 415 nm, respectively. The extracts are intensely colored and very stable at room temperature. The molar absorptivity for DZ-MO and DZ-BPB ion pairs were determined to be 5.3×103 and 6.66×103 L/mol • cm, respectively. The stoichiometry of the reaction was found to be 1:1 in all cases and the conditional stability constant (Kf) of the complexes was calculated. The effective range of concentration for an accurate determination as ascertained from Ringbom's plot was obtained at 16.67-50 μg/mL. The proposed method has been applied successfully to the analysis of drug dosage forms and no interference was observed from common excipients present in pharmaceutical formulations. The results obtained by the proposed method were statistically compared by means of the student t-test and by the variance ratio, and F-test with the HPLC method. Here it is shown to be in excellent agreement with the official method.
Supporting agencies: Financial support from the research affairs of the university of Guilan is acknowledged. The authors are grateful to the Sobhan pharmaceutical company, Rasht, Iran, for the supply of pure drug samples.
About the Authors
M. Mahjoub
Department of Chemistry, University of Guilan
Islamic Republic of Iran
Islamic Republic of Iran
Mohammad Mahjoub.
Rasht
N. Alizadeh
Department of Chemistry, University of Guilan
Islamic Republic of Iran
Islamic Republic of Iran
Nina Alizadeh.
Rasht
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Review
For citations:
Mahjoub M., Alizadeh N. Rapid Spectrophotometric Method for Diazepam Quantification in Pharmaceutical Formulations Using Ion Pair Formation with Methyl Orange and Bromophenol Blue. Zhurnal Prikladnoii Spektroskopii. 2023;90(3):525-1-525-10.
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