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Development and Validation of a Spectrophotometric Method for the Quantitative Determination of Total Triterpenoid and Steroid Compounds in Polypore Fungi
Abstract
Polypore fungi represent a promising source of biologically active triterpenoid and steroid compounds; however, their quantitative determination is complicated by the lack of reference standards as well as the variety and structural similarity of the compounds. In this study, a spectrophotometric method based on the Liebermann–Burchard reaction was developed and validated for the determination of total triterpenes and steroids in polypore extracts. The optimal analytical conditions were established as follows: chloroform as the extraction solvent, extract-to-reagent ratio of 1:1, analytical wavelength of 665 nm, and reaction time of 90 minutes. The method demonstrated satisfactory specificity, accuracy (recoveries of 100.6–108.6%), precision (RSD ≤ 3.4%), and linearity in the range of 31.25–1000 μg/mL (R2 > 0.99). The obtained results confirm the applicability of the proposed approach for the standardization of fungal raw materials and the quality control of polypore-based products.
About the Authors
H. I. Harbatsevich
Belarusian State Medical University
Belarus
Belarus
Minsk
I. A. Komlach
Belarusian State Medical University
Belarus
Belarus
Minsk
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For citations:
Harbatsevich H.I., Komlach I.A. Development and Validation of a Spectrophotometric Method for the Quantitative Determination of Total Triterpenoid and Steroid Compounds in Polypore Fungi. Zhurnal Prikladnoii Spektroskopii. 2026;93(1):78-85. (In Russ.)
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