Synthesis of Zinc Oxide Nanoparticles by Plasma Jet with Pseudomonas Aeruginosa Extract for Inhibiting Pathogenic Bacteria

Zinc oxide nanoparticles were synthesized within 15 min using an argon plasma jet and Pseudomonas aeruginosa bacteria extract. X-ray diffraction confirmed a high-purity hexagonal phase with 26.3 nm as the average crystallite size. Field emission scanning electron microscopy verified the surface topography, revealing spherical particles with sizes up to 46 nm, which was consistent with atomic force microscopy, indicating an average grain size of 46.81 nm. The material’s composition and purity were further verified by energydispersive X-ray spectroscopy, showing no impurities. Fourier transform infrared spectroscopy identified  Zn–O absorption peaks at 676, 608, and 477 cm⁻¹, and UV-Vis spectroscopy revealed an optical bandgap of 3.4 eV. Using both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, the disc diffusion technique was used to assess antibacterial efficiency, demonstrating the inhibition of these bacteria. Overall, this cost-effective method is effective in efficiently synthesizing zinc oxide nanoparticles, making it suitable for developing novel bactericides.

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