Spectrophotometric Estimation of Desidustat: Complex Formation with Iodine and Content Uniformity Test

A simple, sensitive, and cost-effective spectrophotometric method was developed for the quantitative determination of desidustat in pharmaceutical formulations, using molecular iodine as a complexing agent. Desidustat, a hypoxia-inducible factor prolyl hydroxylase inhibitor used to treat anaemia, was analyzed based on the interaction between iodine and a secondary amine group, where coordination occurs through the lone pair of electrons, resulting in the formation of a stable hyperiodine complex. The resulting coloured ion-pair complex exhibited a distinct hyperchromic shift, with maximum absorbance at 235 nm. The proposed method was validated in accordance with the International Council for Harmonisation guidelines. The method demonstrated excellent linearity over the concentration range of 0.25–1.0 μg/L, with a correlation coefficient (r²) of 0.9995. The limits of detection and quantitation were 0.00459 and 0.01378 μg/L, respectively, indicating high sensitivity. Method precision was confirmed through intra- and inter-day studies, yielding mean recovery values of 100.38 and 100.22%, respectively. Accuracy studies showed satisfactory results, with mean percentage recoveries ranging from 99.41 to 99.87%. The validated method was reliable, precise, and suitable for the routine analysis of desidustat in pharmaceutical dosage forms, offering a straightforward alternative to more complex analytical techniques.

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