A NAKED-EYE VISIBLE AND FLUORESCENCE “TURN-ON” PROBE FOR ACETYLCHOLINESTERASE ASSAY AND THE DISCRIMINATION OF BIOTHIOLS
Abstract
We demonstrate the utility of 4-chloro-7-nitrobenzofurazan (NBD-Cl), a simple, low-cost probe, for colorimetrically and fluorescently detecting and differentiating similar thiol species, including cysteine, homocysteine, and glutathione, as well as for assaying acetylcholinesterase (AChE) activity. This assay is a potential tool for investigating thiol functions in disease and diagnosis, and for screening AChE inhibitors. We envision that this versatile probe, which is commercially available and inexpensive, will be useful for further elucidating the roles of thiols in biology and also shows applicability for analysis of AChE inhibitors in physiological samples.
About the Authors
Lingzhi Zhao
Xi’an Medical College; School of Chemistry and Chemical Engineering, Shaanxi Normal University
Russian Federation
Russian Federation
Liu Zhao
Beijing Research Center of Agricultural Standards and Testing
Russian Federation
Russian Federation
Chenxiao Zhang
School of Chemistry and Chemical Engineering, Shaanxi Normal University
Russian Federation
Russian Federation
Yanqi Li
School of Chemistry and Chemical Engineering, Shaanxi Normal University
Russian Federation
Russian Federation
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Review
For citations:
Zhao L., Zhao L., Zhang Ch., Li Ya. A NAKED-EYE VISIBLE AND FLUORESCENCE “TURN-ON” PROBE FOR ACETYLCHOLINESTERASE ASSAY AND THE DISCRIMINATION OF BIOTHIOLS. Zhurnal Prikladnoii Spektroskopii. 2018;85(3):428-435. (In Russ.)
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