RAMAN SPECTRAL IMAGING BASED HISTOPATHOLOGY OF HUMAN SKIN TISSUE
Abstract
Confocal Raman microspectral imaging (CRMI) provides a versatile tool to extract biochemical information on histologically defined regions with improved spatial resolution and sensitivity. Here, we investigate spectral features and perform both uni- and multi-variate analyses to obtain precise correlation between the biochemical profile and histological architecture of ex vivo human skin tissue. The skin dermis layer is visualized by integrated images of collagen peaks at 855, 940, and 1248 cm- 1 , while the stratum corneum layer is described by chemical images of ceramides. Melanin accumulation patterns in epidermis are obtained by integration of 1375 and 1580 cm- 1 Raman peaks. In addition, distinct spectral features of the melanin precursor are revealed by certain Raman vibrations, which structurally characterize the molecular composition and organization of hair follicles. These results offer a unique insight into the skin histology, biochemistry, and biophysics, which provide the possibility of interpreting the underlying mechanisms of skin malignant processes.
About the Authors
Y. . Yin
Northwest University
Russian Federation
Russian Federation
Y. . Gong
Northwest University
Russian Federation
Russian Federation
X. . Zhang
Northwest University
Russian Federation
Russian Federation
J. . Li
Northwest University
Russian Federation
Russian Federation
K. . Wang
Institute of Photonics and Photon-Technology, Northwest University
Russian Federation
Russian Federation
Q. . He
Northwest University
Russian Federation
Russian Federation
J. . Bai
Northwest University
Russian Federation
Russian Federation
J. . Qin
The Second Affiliated Hospital of Xi'an Jiaotong University
Russian Federation
Russian Federation
Sh. . Wang
Institute of Photonics and Photon-Technology, Northwest University
Russian Federation
Russian Federation
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Review
For citations:
Yin Y., Gong Y., Zhang X., Li J., Wang K., He Q., Bai J., Qin J., Wang Sh. RAMAN SPECTRAL IMAGING BASED HISTOPATHOLOGY OF HUMAN SKIN TISSUE. Zhurnal Prikladnoii Spektroskopii. 2018;85(3):518(1)-518(7). (In Russ.)
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