FIRST- AND THIRD-DERIVATIVE SPECTROPHOTOMETRY FOR SIMULTANEOUS DETERMINATION OF DEXAMETHASONE AND CYTARABINE IN PHARMACEUTICAL FORMULATIONS AND BIOLOGICAL FLUIDS
Abstract
The derivative spectrophotometric method was established for simultaneous determination of dexamethasone and cytarabine (cytosine arabinoside). Measurements were made in the zero-crossing wavelengths at 268.0 nm (first derivative) and 264.0 nm (third derivative) for determining dexamethasone and cytarabine, respectively. The calibration graphs were linear in the concentration ranges 0.10 to 10 µg/mL of dexamethasone and 0.25 to 50.0 µg/mL of cytarabine. The limits of detection 0.08 and 0.10 µg/mL and relative standard deviations 3.0 and 1.0% were obtained for dexamethasone and cytarabine, respectively. The possible interfering effect of other substances was also studied to investigate selectivity of the developed method. The proposed method was applied satisfactorily for the simultaneous determination of both drugs in the pharmaceutical formulation and biological fluid samples.
Keywords
dexamethasone,
cytarabine,
derivative spectrophotometry,
pharmaceutical formulation,
biological fluid
Supporting agencies: The authors thank Islamic Azad University (IAU), Yazd Branch, Yazd, Iran for the financial support of this research project.
About the Authors
V. Montazeralmahdi
Yazd Branch, Islamic Azad University
Islamic Republic of Iran
Islamic Republic of Iran
Department of Chemistry
Yazd
A. Sheibani
Yazd Branch, Islamic Azad University
Islamic Republic of Iran
Islamic Republic of Iran
Department of Chemistry
Yazd
M. Reza Shishehbore
Yazd Branch, Islamic Azad University
Islamic Republic of Iran
Islamic Republic of Iran
Department of Chemistry
Yazd
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Review
For citations:
Montazeralmahdi V., Sheibani A., Reza Shishehbore M. FIRST- AND THIRD-DERIVATIVE SPECTROPHOTOMETRY FOR SIMULTANEOUS DETERMINATION OF DEXAMETHASONE AND CYTARABINE IN PHARMACEUTICAL FORMULATIONS AND BIOLOGICAL FLUIDS. Zhurnal Prikladnoii Spektroskopii. 2019;86(5):760-764.
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