COMPLEX ANALYSIS OF FLUORESCENCE INTENSITY FLUCTUATIONS OF MOLECULAR COMPOUNDS
Abstract
A method is proposed for the complex analysis of fluctuations in the fluorescence intensity of molecular compounds, which allows determining the structural composition of protein oligomers. The idea of the method is to analyze the photon counting histograms of experimental measurements using principal component analysis to assess the presence of oligomeric compounds, and to perform hierarchical cluster analysis, to determine the data classes corresponding to various molecular compounds, followed by selecting cluster medoids to determine the oligomeric composition of protein complexes. The efficiency of the analysis algorithms developed within the framework of the proposed method was confirmed on simulated and experimental photon counting histograms of the measured fluorescence intensity fluctuations of monomeric and dimeric forms of green fluorescent protein (GFP).
About the Authors
M. M. Yatskou
Department of Systems Analysis and Computer Modelling, Belarusian State University
Belarus
Minsk, 220030
Belarus
Minsk, 220030
V. V. Skakun
Department of Systems Analysis and Computer Modelling, Belarusian State University
Belarus
Minsk, 220030
Belarus
Minsk, 220030
L. Nederveen-Schippers
University of Groningen
Netherlands
9747AG Groningen
Netherlands
9747AG Groningen
A. Kortholt
University of Groningen
Netherlands
9747AG Groningen
Netherlands
9747AG Groningen
V. V. Apanasovich
Institute of IT&Business Administration
Belarus
Minsk, 220004
Belarus
Minsk, 220004
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Review
For citations:
Yatskou M.M., Skakun V.V., Nederveen-Schippers L., Kortholt A., Apanasovich V.V. COMPLEX ANALYSIS OF FLUORESCENCE INTENSITY FLUCTUATIONS OF MOLECULAR COMPOUNDS. Zhurnal Prikladnoii Spektroskopii. 2020;87(4):628-636. (In Russ.)
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