CTAB-CAPPED GOLD NANOPARTICLES AS A NEW PROBE FOR SPECTROPHOTOMETRIC DETERMINATION OF HEPARIN
Abstract
A simple, fast, and sensitive spectrophotometric method is reported for the determination of heparin concentration in human plasma samples. The assay is based on enhancing the extinction intensity of CTAB-capped gold nanoparticles in the presence of heparin molecules. The strong electrostatic interaction of the positively-charged gold nanoparticles with the negatively-charged heparin molecules induces a marked increase in size of gold nanoparticles, which results in a dramatic rise in the extinction spectrum of gold nanoparticles. Since the enhanced extinction intensity at 528 nm is proportional to the concentration of heparin, quantitative determination of heparin is possible. Under optimum conditions, the enhanced extinction intensity is linearly correlated with the concentration of heparin in the range 0.18-11.98 U/mL with limit of detection 0.08 U/mL.
About the Authors
R. . Faham
Marand Branch of Islamic Azad University
Russian Federation
Russian Federation
A. . Samadi
Tabriz Branch of Islamic Azad University
Russian Federation
Russian Federation
J. . Abolhasani
Tabriz Branch of Islamic Azad University
Russian Federation
Russian Federation
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Review
For citations:
Faham R., Samadi A., Abolhasani J. CTAB-CAPPED GOLD NANOPARTICLES AS A NEW PROBE FOR SPECTROPHOTOMETRIC DETERMINATION OF HEPARIN. Zhurnal Prikladnoii Spektroskopii. 2017;84(3):404-409. (In Russ.)
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