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APPLICATION OF RAMAN SPECTROSCOPY FOR STUDYING THE MECHANISMS OF NEUTROPHIL ACTIVATION BY CARBON NANOTUBES
Abstract
Using the Raman spectroscopy method we determined the mechanisms of neutrophil activation under the interaction with multi-walled сarbon nanotubes (MWCNTs) functionalized by the COOH groups (MWCNT-COOH) and in complexes with DNA (MWCNT-COOH/DNA). It was revealed that short (100–300 nm) MWCNT-COOH and MWCNT-COOH/DNA, when interacting with neutrophils, cause cytoskeleton reorganization, namely, they activate G-actin polymerization to F-actin in cells. Raman spectroscopy revealed that the investigated CNTs are not phagocytosed by neutrophils. MWCNT-COOH enhance the generation of reactive oxygen and chlorine species by neutrophils, which is confirmed by increasing the NADPH oxidase activity in the cell suspension. The interaction of neutrophils with MWCNT-COOH/DNA leads to the inhibition of the phagocyte oxygen-activating ability and to the formation of neutrophilic extracellular traps with the inclusion of carbon nanostructures. The free radical products generated by neutrophils disrupt the crystal structure of MWCNTs.
About the Authors
L. N. Golubewa
Institute for Nuclear Problems of Belarusian State University
Belarus
Minsk, 220006
Belarus
Minsk, 220006
T. A. Kulahava
Institute for Nuclear Problems of Belarusian State University;
Belarusian State University
Belarus
Belarus
Minsk, 220006;
Minsk, 220030
Y. S. Leonik
Belarusian State University
Belarus
Minsk, 220030
Belarus
Minsk, 220030
M. V. Shuba
Institute for Nuclear Problems of Belarusian State University
Belarus
Minsk, 220006
Belarus
Minsk, 220006
G. N. Semenkova
Belarusian State University
Belarus
Minsk, 220030
Belarus
Minsk, 220030
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Review
For citations:
Golubewa L.N., Kulahava T.A., Leonik Y.S., Shuba M.V., Semenkova G.N. APPLICATION OF RAMAN SPECTROSCOPY FOR STUDYING THE MECHANISMS OF NEUTROPHIL ACTIVATION BY CARBON NANOTUBES. Zhurnal Prikladnoii Spektroskopii. 2021;88(1):87-95. (In Russ.)
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