FLUORESCENCE QUENCHING OF CARBOXYFLUORESCEINS COVALENTLY CONJUGATED WITH OLIGONUCLEOTIDES

The dynamic and static quenching of 6-(2',7'-dimethoxy-4',5'-dichloro)carboxyfluorescein (JOE) by nucleosides (deoxyadenosine, deoxycytidine, deoxyguanosine, thymidine, and deoxycyridine) in the tris-acetate buffer solution was analyzed using the Stern-Volmer equation. Only one of the five nucleosides - deoxyguanosine - was found to exhibit predominantly static quenching. The quantum yields of the fluorescence of 5- and 6-carboxyfluorescein (FAM), as well as of 5- and 6-JOE, covalently bound by a rigid (4-transaminocyclohexanol) linker to the oligonucleotide, is greater than for their analogues with flexible (6-aminohexanol) linker. It is shown that quenching of fluorescence in systems with a flexible linker takes place mainly with the van der Waals contact of the fluorophore with guanine. An increase in the number of consecutively located guanines at the 5'-end linked to the dye in the composition of oligonucleotides and their duplexes results in a decrease in the quantum yield of fluorescence. Quantum-chemical calculations show a shift of the absorption and fluorescence spectra of 5-FAM and 5-JOE isomers to the low-frequency region compared to 6-isomers

дезоксирибоолигонуклеотид, ДНК-дуплекс, спектр поглощения, флуоресценция, квантовый выход, тушение флуоресценции, перенос электрона, deoxyribooligonucleotide, duplex, absorption spectra, fluorescence, quantum yield, quenching of fluorescence, electron transfe

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