FLUORESCENCE DYNAMICS OF N-TERMINAL TRYPTOFAN-X RESIDUES IN POLYPEPTIDE: pH RESPONSE
Abstract
The influence of the second amino acid on the pH response of the genetically encoded pH indicators is studied. The second Trp has been substituted by Ala and Glu to reduce self-quenching. Compared with the previously reported Trp-Trp-Ala-Ser (WWAS), the two new tetrapeptides, Trp-Ala-Ala-Ser (WAAS) and Trp-Glu-Ala-Ser (WEAS), have higher fluorescence quantum yields, longer fluorescence lifetimes, and more sensitive pH responses. As novel genetically encoded pH indicators, the N-terminal Trp-Ala and Trp-Glu could be fused to proteins for monitoring the environmental pH values during the studies of functional proteins.
About the Authors
L. . Li
East China Normal University, State Key Laboratory of Precision Spectroscopy; School of Science, Jiangsu Provincial Research Center of Light Industrial Optoelectronic Engineering and Technology, Jiangnan University
Russian Federation
Russian Federation
X. . Cao
East China Normal University, State Key Laboratory of Precision Spectroscopy
Russian Federation
Russian Federation
Zh. . Zhou
East China Normal University, State Key Laboratory of Precision Spectroscopy
Russian Federation
Russian Federation
S. . Zhang
East China Normal University, State Key Laboratory of Precision Spectroscopy; Collaborative Innovation Center of Extreme Optics, Shanxi University
Russian Federation
Russian Federation
H. . Pan
East China Normal University, State Key Laboratory of Precision Spectroscopy
Russian Federation
Russian Federation
J. . Chen
East China Normal University, State Key Laboratory of Precision Spectroscopy
Russian Federation
Russian Federation
J. . Xu
East China Normal University, State Key Laboratory of Precision Spectroscopy; Collaborative Innovation Center of Extreme Optics, Shanxi University
Russian Federation
Russian Federation
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Review
For citations:
Li L., Cao X., Zhou Zh., Zhang S., Pan H., Chen J., Xu J. FLUORESCENCE DYNAMICS OF N-TERMINAL TRYPTOFAN-X RESIDUES IN POLYPEPTIDE: pH RESPONSE. Zhurnal Prikladnoii Spektroskopii. 2017;84(4):610-615. (In Russ.)
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