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Fluorescent Characteristics of Hoechst 33258 Complexes with Serum Albumin and DNA
https://doi.org/10.47612/0514-7506-2022-89-4-519-524
Abstract
We study the interaction of the bisbenzimodazole compound Hoechst 33258 with bovine serum albumin and calf thymus DNA by the fluorescence spectroscopy method. The fluorescence spectra of the complexes are obtained. Based on their maxima, the dependence curves of the fluorescence intensities of complexes of this ligand with protein and DNA on the ratio r = macromolecule/ligand are constructed. It is shown that the dependence curve in the case of albumin decreases at low protein concentrations and increases at its high concentrations. In the case of DNA, this curve increases significantly. This fact is due to the binding of the ligand to both DNA and albumin.
About the Authors
A. P. Antonyan
Yerevan State University
Armenia
Armenia
Yerevan
M. A. Parsadanyan
Yerevan State University
Armenia
Armenia
Yerevan
N. H. Petrosyan
Yerevan State University
Armenia
Armenia
Yerevan
P. O. Vardevanyan
Yerevan State University
Armenia
Armenia
Yerevan
References
1. А. T. Buddanavar, Sh. T. Nandibewoor. J. Pharm. Anal., 7 (2017) 148—155
2. H. Ojha, Bh. M. Murari, S. Anand, M. I. Hassan, F. A. N. K. Chaudhury. Chem. Pharm. Bull., 57, N 5 (2009) 481—486
3. X.-X. Zhang, Sh. L. Brantley, S. A. Corcelli, A. Tokmakoff. Comm. Biol., 3 (2020) 1—9
4. A. J. Angelbello, J. L. Childs-Disney, P. Zhang, Z.-F. Wang, M. D. Disney. Chem. Rev., 118, N 4 (2018) 1599—1663
5. R. Fritzsch, P. M. Donadson, G. M. Greetham, M. Towrie, A. W. Parker, M. J. Baker, N. T. Hunt. Anal. Chem., 90, N 4 (2018) 2732—2740
6. K. A. Majorek, P. J. Porebski, A. Dayal, M. D. Zimmerman, K. Jablouska, A. J. Stewart. Mol. Immunol., 52, N 3 (2012) 174—182
7. J. Bucevicius, G. Lukinavicius, R. Gerasimaite. Chemosensors, 6, N 18 (2018) 1—12
8. P. O. Vardevanyan, M. A. Parsadanyan, A. P. Antonyan, M. A. Shahinyan, A. T. Karapetian. J. Biomol. Struct. Dyn., 39, N 4 (2021) 1519—1523
9. P. O. Vardevanyan., A. P. Antonyan, M. A. Parsadanyan, K. V. Pirumyan, A. M. Muradyan, A. T. Karapetian. J. Biomol. Struct. Dyn., 25, N 6 (2008) 641—646
10. P. O. Vardevanyan, M. A. Parsadanyan, A. P. Antonyan, M. A. Shahinyan. J. Biomol. Struct. Dyn., 40, N 3 (2020) 1182—1188, doi: 10.1080/07391102.2020.1823883
11. Z.-Y. Tian, Li-Na Song, Y. Zhao, F.-L. Zang, Zh.-H. Zhao, N.-H. Chen, X.-J. Xu, Ch.-J. Wang. Molecules, 20 (2015) 16491—16523
12. R. Choudhury, A. K. Sharma, P. Paudel, P. Wilson, A. B. Pereira. Anal. Biochem., 646, Article N 114630, doi: 10.1016/j.ab.2022.114630
13. M. Ronzetti, B. Baljinnyam, A Yesgar, A. Simeonov. Expert Opinion Drug Dis., 13 (2018) 1005—1014
14. W. Yang, C. Liu, S. Lu, S. Cheng, J. Du, Q. Gao, P. Shen, H. Luo, Y. Liu, C. Yang. J. Lumin., 192 (2017) 478—485
15. S. I. Reja, L. A. Khan, V. Bhalla, M. Kumar. Chem. Commun., 52 (2016) 1182—1185
16. А. П. Антонян, Н. Г. Петросян, П. О. Вардеванян. Журн. прикл. спектр., 88, № 6 (2021) 942—947 [A. P. Antonyan, N. H. Petrosyan, P. O. Vardevanyan. J. Appl. Spectr., 88, N 6 (2021) 1210—1214]
17. J. R. Lakowicz. Principles of Fluorescence Spectroscopy, 3rd ed., University of Maryland, School of Medicine Baltimore, Maryland, USA, Printed in Singapore (KYO) (2006) 16—17
Review
For citations:
Antonyan A.P., Parsadanyan M.A., Petrosyan N.H., Vardevanyan P.O. Fluorescent Characteristics of Hoechst 33258 Complexes with Serum Albumin and DNA. Zhurnal Prikladnoii Spektroskopii. 2022;89(4):519-524. (In Russ.) https://doi.org/10.47612/0514-7506-2022-89-4-519-524
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