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STUDY OF ADRENALIN AUTOOXIDATION WITH UV-VIS SPECTROSCOPY METHODS
https://doi.org/10.47612/0514-7506-2022-89-5-701-708
Abstract
The process of adrenaline autoxidation in an alkaline medium (pH=10.65) in the presence of sulfite was experimentally studied by UV-Vis spectroscopy. The absorption spectra of the final product of autoxidation (λm = 290–300 nm, εm = 8400 M–1 · cm–1), the intermediate adduct of adrenochrome with sulfite (λm =343 nm, εm = 68000 M–1 · cm–1), and the reactive an intermediate product, presumably adrenolutin (λm = 311, εm = =140000 M–1 · cm–1 and λm=373 nm, εm =129000 M–1 · cm–1) were determined with experimental data processing. The estimated equilibrium constant for the formation of adrenochrome adduct with sulfite 5000 M-1 agrees with the literature data for carbonyl compounds. The results obtained explain the contradictions in the results of various authors concerning the oxidation and autoxidation of adrenaline in an alkaline medium.
About the Authors
M. A. Ivanova
State Medical University
Russian Federation
Russian Federation
Kemerovo
E. K. Nefyodova
State Medical University
Russian Federation
Russian Federation
Kemerovo
E. M. Maltseva
State Medical University
Russian Federation
Russian Federation
Kemerovo
A. V. Kalenskii
Kemerovo State University
Russian Federation
Russian Federation
Kemerovo
A. A. Zvekov
State Medical University; Kemerovo State University
Russian Federation
Russian Federation
Kemerovo
References
1. R. Alhasan, D. Njus. Anal. Biochem., 381 (2008) 142—147
2. S. Garnayak, S. Patel. Ind. Eng. Chem. Res., 53 (2014) 12249—12256
3. M. Z. A. Rafiquee, M. R. Siddiqui, M. S. Ali, H. A. Al-Lohedan. Spectrochim. Acta A, 126 (2014) 21—27
4. L. Hu, N. Gao, J. Shan, X. Wang, H. Wang, X. Yang, H. Ma, Q. Wei. Langmuir, 36, N 18 (2020) 5040—5047
5. Е. И. Рябинина, Е. Е. Зотова, Е. Н. Ветрова, Н. И. Пономарева, Т. Н. Илюшина. Химия растит. сырья, № 3 (2011) 117—121
6. T. V. Sirota. Biophysics, 65, N 4 (2020) 548–556
7. Т. В. Сирота. Способ определения антиоксидантной активности супероксиддисмутазы и химических соединений, патент РФ 2 144 674, заявка 99103194/14 (1999)
8. E. A. Guzov, V. N. Kazin, V. A. Moshareva, A. A. Zhukova. J. Appl. Spectr., 85, N 6 (2019) 1107–1113
9. I. Cs. Szigyártó, L. Szabó, L. I. Simándi. J. Mol. Cat. A, 372 (2013) 66—71
10. J. Brustugun, S. Kristensen, H.H. Tønnesen. Pharmazie, 59, N 6 (2004) 457—463
11. S. I. Baskin, G. S. Behonick, R. J. Schafer, M. J. Novak, C. M. Arroyo. Toxic Subst. Mech., 19 (2000) 239—252
12. M. P. Rigobello, G. Scutari, R. Boscolo, A. Bindoli. Biol. and Chem., 5, N 1 (2001) 39—46
13. A. Palumbo, M. d'Ischia, G. Misuraca, G. Prota. Biochim. Biophys. Acta, 990 (1989) 297—302
14. S. Corona-Avendaño, G. Alarcón-Angeles, A. Rojas-Hernández, M. A. Romero-Romo, M. T. Ramirez-Silva. Spectrochim. Acta A, 61 (2005) 305—311
15. И. Я. Берштейн, Ю. Л. Каминский. Спектрофотометрический анализ в органической химии, Ленинград, Химия (1975) 26—45
16. M. D. Hawley, S. V. Tatawawadi, S. Piekarski, R. N. Adams. J. Am. Chem. Soc., 89, N 2 (1967) 447—450
17. P. Guthrie, Y.-Y. Wu, A. R. Bannister, S. Peiris, I. Povar, E.A. Wilson, Q. Wang. Can. J. Chem., 93 (2015) 227—233
18. P. Neta, R. E. Huie. Env. Health Pers., 64 (1985) 209—217
Review
For citations:
Ivanova M.A., Nefyodova E.K., Maltseva E.M., Kalenskii A.V., Zvekov A.A. STUDY OF ADRENALIN AUTOOXIDATION WITH UV-VIS SPECTROSCOPY METHODS. Zhurnal Prikladnoii Spektroskopii. 2022;89(5):701-708. (In Russ.) https://doi.org/10.47612/0514-7506-2022-89-5-701-708
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