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TWO-DIMENSIONAL AND THREE-DIMENSIONAL RAMAN SPECTROSCOPIC IMAGING OF Spirulina platensis CELLS
Abstract
Two-dimensional (2D) and 3D Raman spectroscopic imaging of Spirulina platensis was carried out to investigate its applicability in studying the chemical distribution and morphology of algal cells. The Spirulina trichome was easily damaged by laser illumination unless the laser parameters were properly adjusted. Raman spectra exhibited an evident sign of fluorescence, which changed during cumulative laser illumination on the same spot. Six peaks were selected from the spectra for chemical mapping, which showed distinct cellular features, including the central and peripheral parts of the cytoplasm, cell wall, sheath, and medium residues. Possible chemicals/organelles in these parts and mechanisms were discussed.
Supporting agencies: We gratefully thank the Educational and Scientific Research Program for Young Scholars Sponsored by the Educational Department of Fujian Province (JAT200733) and the Emerging and Future Industry Development Funds in Shenzhen (20170503163617880) for funding this study. This study was funded by the Educational and Scientific Research Program for Young Scholars Sponsored by the Educational Department of Fujian Province (JAT200733) and the Emerging and Future Industry Development Funds in Shenzhen (20170503163617880).
About the Authors
S. Zhang
Engineering Research Center of Natural Cosmeceuticals College of Fujian Province, Xiamen Medical College; Department of Public Health and Medical Technology, Xiamen Medical College
China
China
Shudi Zhang
Xiamen, Fujian
S. Gao
Engineering Research Center of Natural Cosmeceuticals College of Fujian Province, Xiamen Medical College; Department of Public Health and Medical Technology, Xiamen Medical College
China
China
Shuitian Gao
Xiamen, Fujian
F. Chen
Engineering Research Center of Natural Cosmeceuticals College of Fujian Province, Xiamen Medical College; Department of Public Health and Medical Technology, Xiamen Medical College
China
China
Fangfang Chen
Xiamen, Fujian
H. Pang
Engineering Research Center of Natural Cosmeceuticals College of Fujian Province, Xiamen Medical College; Department of Public Health and Medical Technology, Xiamen Medical College
China
China
Haiyue Pang
Xiamen, Fujian
Y. Gao
Shenzhen Space Food Analysis and Test Center Co. Ltd
China
China
Yanfen Gao
Shenzheng, Guangdong
G. Wang
Engineering Research Center of Natural Cosmeceuticals College of Fujian Province, Xiamen Medical College; Department of Public Health and Medical Technology, Xiamen Medical College
China
China
Gueyhorng Wang
Xiamen, Fujian
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Review
For citations:
Zhang S., Gao S., Chen F., Pang H., Gao Y., Wang G. TWO-DIMENSIONAL AND THREE-DIMENSIONAL RAMAN SPECTROSCOPIC IMAGING OF Spirulina platensis CELLS. Zhurnal Prikladnoii Spektroskopii. 2022;89(5):741.
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