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DESIGN OF A FLUORESCENCE ENHANCED APTASENSOR FOR SENSITIVE DETECTION OF SILVER IONS
Abstract
A highly sensitive fluorescence-enhanced aptasensor was designed to detect silver ions (Ag+) using metal-enhanced fluorescence. Interaction of Ag+ with cytosine nucleobases was used to achieve a low detection limit. Aptamer-modified gold nanoparticles (Au NPs) were mixed with 6-carboxyfluorescein (FAM)-labeled DNA to prepare the sensor. In a solution without Ag+, aptamer and FAM-labeled DNA strands remained free because of repulsion between cytosine. In the presence of Ag+, pairs of aptamer and FAM-labeled DNA strands formed double helices through cytosine–Ag+–cytosine interactions. These interactions brought FAM close to the Au NPs. The number of adenines repeats in the aptamer was altered to adjust the distance between the Au NPs and FAM, and provide controllable localized surface plasmon resonance. With the optimum number of adenine repeats (n = 24), the linear range for detection of Ag+ was 0.694 to 6.94 nmol/L and the detection limit was 0.694 nmol/L. The aptasensor showed excellent specificity and gave a strong detection signal for Ag+ present at trace concentrations to overcome issues associated with the detection of weak signals.
Supporting agencies: This research was supported by the National Natural Science Foundation of China (grant Nos. 62073299 and 61801436), the Project of Central Plains Science and Technology Innovation Leading Talents (grant No. 224200510026), the Program for Innovative Research Team (in Science and Technology) in Henan University (grant No. 20IRTSTHN017), the Science and Technology project of Henan Province (grants Nos. 212102210196 and 212102210278).
About the Authors
L. Ren
School of Electrical and Information Engineering at Zhengzhou University of Light Industry
China
China
Linjiao Ren
Zhengzhou
G. Chen
School of Electrical and Information Engineering at Zhengzhou University of Light Industry
China
China
Gaokai Chen
Zhengzhou
Z. Peng
School of Electrical and Information Engineering at Zhengzhou University of Light Industry
China
China
Zheng Peng
Zhengzhou
X. Xu
School of Electrical and Information Engineering at Zhengzhou University of Light Industry
China
China
Xiaoping Xu
Zhengzhou
P. Zhang
School of Electrical and Information Engineering at Zhengzhou University of Light Industry
China
China
Pei Zhang
Zhengzhou
Z. Qin
School of Electrical and Information Engineering at Zhengzhou University of Light Industry
China
China
Zirui Qin
Zhengzhou
Q. Chen
School of Electrical and Information Engineering at Zhengzhou University of Light Industry
China
China
Qinghua Chen
Zhengzhou
Y. Yan
School of Electrical and Information Engineering at Zhengzhou University of Light Industry
China
China
Yanxia Yan
Zhengzhou
L. Jiang
School of Electrical and Information Engineering at Zhengzhou University of Light Industry
China
China
Liying Jiang
Zhengzhou
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Review
For citations:
Ren L., Chen G., Peng Z., Xu X., Zhang P., Qin Z., Chen Q., Yan Y., Jiang L. DESIGN OF A FLUORESCENCE ENHANCED APTASENSOR FOR SENSITIVE DETECTION OF SILVER IONS. Zhurnal Prikladnoii Spektroskopii. 2022;89(5):745.
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