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Optimization of Structural and Optical Parameters of Plasmon Silver Films for IgG-FITC Fluorescent Analysis
https://doi.org/10.47612/0514-7506-2023-90-1-48-55
Abstract
Optimization of the fluorescence enhancement factor of the conjugate of immunoglobulin G labeled with fluorescein isothiocyanate (IgG-FITC) as an immunofluorescent marker was carried out depending on the optical and topographic parameters of a colloidal silver film on the surface of a standard polystyrene plate for immunoassay. Factors influencing fluorescence enhancement were identified. Using time-resolved spectroscopy, analysis of fluorescence excitation spectra, and enzyme immunoassay determination of the relative concentrations of IgG-FITC adsorbed on the solid phase, it was shown that fluorescence enhancement is a plasmon-resonance process. The most important parameter correlated with the fluorescence enhancement factor is the optical density of silver colloidal film at the excitation and emission wavelengths of IgG-FITC. The maximum enhancement factor of 10.2 times was obtained for silver films with the highest optical density.
About the Authors
O. S. Kulakovich
B. I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus
Belarus
Belarus
Minsk
A. A. Shcherbovich
International Sakharov Environmental Institute of Belarusian State University
Belarus
Belarus
Minsk
A. A. Ramanenka
B. I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus
Belarus
Belarus
Minsk
I. V. Koktysh
International Sakharov Environmental Institute of Belarusian State University
Belarus
Belarus
Minsk
Ya. I. Melnikova
International Sakharov Environmental Institute of Belarusian State University
Belarus
Belarus
Minsk
S. V. Gaponenko
B. I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus
Belarus
Belarus
Minsk
S. A. Maskevich
International Sakharov Environmental Institute of Belarusian State University
Belarus
Belarus
Minsk
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Review
For citations:
Kulakovich O.S., Shcherbovich A.A., Ramanenka A.A., Koktysh I.V., Melnikova Ya.I., Gaponenko S.V., Maskevich S.A. Optimization of Structural and Optical Parameters of Plasmon Silver Films for IgG-FITC Fluorescent Analysis. Zhurnal Prikladnoii Spektroskopii. 2023;90(1):48-55. (In Russ.) https://doi.org/10.47612/0514-7506-2023-90-1-48-55
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