|Generate QR code
Open Access
Subscription Access
Computational Simulation, Multi-Spectroscopic and Degradation Analysis of the Interaction between Trametes versicolor Laccase and Bisphenol E
Abstract
Trametes versicolor laccase, one of the main enzymes used for the biodegradation of environmental pollutants, has received much attention in the degradation of phenolic pollutants. In this study, the binding energy between the Trametes versicolor laccase and bisphenol E (BPE) is first calculated by means of computational simulation. Moreover, the interaction between Trametes versicolor laccase and bisphenol E is studied with multi-spectroscopy. The results show that bisphenol E can be effectively degraded by crude Trametes versicolor laccase under optimal incubation conditions. The kinetic study is used to characterize the kinetic features of the laccase-catalytic degradation of BPE. The calculation results suggest that the reaction can proceed spontaneously. Spectral analyses show that the secondary structure oflaccaseis changed after the interaction between laccase and bisphenol E. The degradation efficiency of BPE is up to 93.64% after reacting for 6 h, and the maximum catalytic reaction rate is 0.1764 mg/(L·min). The reactions follow a first-order kinetic equation when the initial concentration of the substrate is lower than 5 mol/L.
Keywords
Supporting agencies: This work is financially supported by the Research funds of the Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials (No. EMFM20211113), the National Natural Science Foundation of China (Grant No. 31860251 and No. 21866011) and the Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control (No. 1701K003).
About the Authors
Xiaolian Lin
Guangxi Key Laboratory of Electrochemical and Magneto-Chemical Functional Materials;
College of Chemistry and Bioengineering, Guilin University of Technology
China
China
Guilin
Hongyan Liu
Guangxi Key Laboratory of Electrochemical and Magneto-Chemical Functional Materials;
College of Chemistry and Bioengineering, Guilin University of Technology
China
China
Guilin
Minhua Xu
Guangxi Key Laboratory of Electrochemical and Magneto-Chemical Functional Materials;
College of Chemistry and Bioengineering, Guilin University of Technology
China
China
Guilin
Mengjie Shi
Guangxi Key Laboratory of Electrochemical and Magneto-Chemical Functional Materials;
College of Chemistry and Bioengineering, Guilin University of Technology
China
China
Guilin
Zhongsheng Yi
College of Chemistry and Bioengineering, Guilin University of Technology
China
China
Guilin
Litang Qin
The Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control
China
China
Guilin
Huiying Chen
College of Chemistry and Bioengineering, Guilin University of Technology
China
China
Guilin
References
1. V. W. Makene, E. J. Pool, Int. J. Environ. Res. Public. Health, 16 (2019).
2. A. E. Hipwell, L. G. Kahn, P. Factor-Litvak, C. A. Porucznik, E. L. Siegel, R. N. Fichorova, R. F. Hamman, M. Klein-Fedyshin, K. G. Harley, Human Rep. Update, 25, 51–71 (2019).
3. Q. Zhang, C. Ji, X. Yin, L. Yan, M. Lu, M. Zhao, Environ. Poll., 210, 27–33 (2016).
4. 4. G. H. Wang, F. Wu, Environ. Chem., 25, 458–461 (2006).
5. M. Y. Chen, M. Ike, M. Fujita, Environ. Toxic., 17, 80–86 (2002).
6. M. Xiao, J. Q. Xiao, Water Purif. Technol., 28, 21–24 (2009).
7. C. Y. Bao, Y. Wang, X. L. Xu, D. Li, J. Chen, Z. B. Guan, B. Y. Wang, M. Hong, J. Y. Zhang,
8. T. H. Wang, Q. Zhang, Bioresour. Technol., 342, 126026 (2021).
9. R. C. Minussi, G. M. Pastore, N. Duran, Bioresour. Technol., 98, 158–164 (2007).
10. C. Zhang, L. Liu, G.-M. Zeng, D.-L. Huang, C. Lai, C. Huang, Z. Wei, N.-J. Li, P. Xu, M. Cheng, F.-L. Li, X.-X. He, M.-Y. Lai, Y.-B. He, Biochem. Eng. J., 91, 149–156 (2014).
11. G. Benfield, S. M. Bocks, K. Bromley, B. R. Brown, Phytochemistry, 3, 79–88 (1964).
12. J. A. Majeau, S. K. Brar, R. D. Tyagi, Bioresour. Technol., 101, 2331–2350 (2010).
13. A. C. Mot, R. Silaghi-Dumitrescu, Biochemistry (Mos.), 77, 1395–1407 (2012).
14. L. Munk, A. K. Sitarz, D. C. Kalyani, J. D. Mikkelsen, A. S. Meyer, Biotechnol. Adv., 33, 13–24 (2015).
15. H. Catherine, M. Penninckx, D. Frédéric, Environ. Technol. Innovat., 5, 250–266 (2016).
16. G. Macellaro, C. Pezzella, P. Cicatiello, G. Sannia, A. Piscitelli, Biomed. Res. Int., 614038 (2014).
17. U. N. Dwivedi, P. Singh, V. P. Pandey, A. Kumar, J. Mol. Catal. B Enzym., 68, 117–128 (2011).
18. M. Asgher, A. Wahab, M. Bilal, H. M. N. Iqbal, Waste Biomass Valorization, 9, 2071–2079 (2017).
19. S.Beck,E.Berry,S.Duke,A.Milliken,H.Patterson,D.L.Prewett,T.C.Rae,V.Sridhar, N. Wendland, B. W. Gregory, C. M. Johnson, Int. Biodeterior. Biodegradation, 127, 146–159 (2018).
20. R. Bourbonnais, M. G. Paice, Appl. Microbiol. Biotech., 36, 823–827 (1992).
21. D. Daâssi, A. Prieto, H. Zouari-Mechichi, M. J. Martínez, M. Nasri, T. Mechichi, Int. Biodeterior. Biodegradation, 110, 181–188 (2016).
22. M. Maryskova, I. Ardao, C. A. Garcia-Gonzalez, L. Martinova, J. Rotkova, A. Sevcu, Enzyme Microbiol. Technol., 89, 31–38 (2016).
23. L. Hongyan, Z. Zexiong, X. Shiwei, X. He, Z. Yinian, L. Haiyun, Y. Zhongsheng, Chemosphere, 224, 743–750 (2019).
24. K. Piontek, M. Antorini, T. Choinowski, J. Biol. Chem., 277, 37663–37669 (2002).
25. X. Hou, J. Du, J. Zhang, L. Du, H. Fang, M. Li, J. Chem. Inf. Model., 53, 188–200 (2013).
26. A. P. Norgan, P. K. Coffman, J. P. A. Kocher, D. J. Katzmann, C. P. Sosa, J. Cheminformatics, 3, 12 (2011).
27. M. A. Murcko, J. Med. Chem., 38, No. 26, 4953–4967 (1995).
28. Y. M. Cao, L. Xu, L. Y. Jia, N. Biotechnol., 29, 90–98 (2011).
29. R. A. Laskowski, M. B. Swindells, J. Chem. Inf. Model., 51, 2778–2786 (2011).
30. Y. Wei, Z. Yi, J. Xu, W. Yang, L. Yang, H. Liu, J. Biomol. Struct. Dyn., 37, 1402–1413 (2019).
31. 30. J. Xu, Y. Wei, W. Yang, L. Yang, Z. Yi, Analyst, 143, 4662–4673 (2018).
32. D. Wu, D. Liu, Y. Zhang, Z. Zhang, H. Li, Eur. J. Med. Chem., 146, 245–250 (2018).
33. A. E. Illera, S. Beltran, M. T. Sanz, Sci. Rep., 9, 13749 (2019).
34. Y. M. Song, J. Wu, X. R. Zheng, Q. Wu, Chin. J. Inorg. Chem., 22, 1615–1622 (2006).
35. PrinciplesofFluorescenceSpectroscopy,Ed.R.L.Joseph,SpringerScience&BusinessMedia, 529–569 (2013).
36. I. P. Caruso, W. Vilegas, M. A. Fossey, M. L. Cornelio, Spectrochim. Acta A Mol. Biomol. Spectrosc., 97, 449–455 (2012).
37. Z. A. Parray, F. Ahmad, M. I. Hassan, I. Hasan, A. Islam, ACS Omega, 5, 13840–13850 (2020).
38. B. Nian, C. Cao, Y. Liu, J. Chem. Technol. Biotech., 95, 86–93 (2019).
39. S. K. Pawar, S. Jaldappagari, J. Pharm. Anal., 9, 274–283 (2019).
40. S. K. Pawar, R. Punith, R. S. Naik, J. Seetharamappa, J. Biomol. Struct. Dyn., 35, 3205–3220 (2017).
41. T. A. Wani, A. H. Bakheit, M. A. Abounassif, S. Zargar, Front Chem., 6, 47 (2018).
42. S. Li, J. H. Zhang, X. Y. Li, J. Q. Fu, J. Beijing Institute Clothing Technol., 31, 68–72 (2011).
43. G. Konstantin, I. Aymelt, S. G. Roger, G. Klaus, K. Carsten, Biophys. Comp. Biol., 110, 13380–13385 (2013).
44. Y. G. Shi, Q. Q. Guo, X. W. Yang, X. F. Liu, S. J. Wang, J. Chin. Institute Food Sci. Tech., 18, 225–231 (2018).
45. H. Tang, D. Zhao, Bioorg. Chem., 88, 102981 (2019).
46. Y. J. Kim, J. A. Nicell, Bioresour. Technol., 97, 1431–1442 (2006).
47. I. Escalona, J. de Grooth, J. Font, K. Nijmeijer, J. Membrane Sci., 468, 192–201 (2014).
48. J. Margot, J. Maillard, L. Rossi, D. A. Barry, C. Holliger, New Biotech., 30, 803–813 (2013).
Review
For citations:
Lin X., Liu H., Xu M., Shi M., Yi Zh., Qin L., Chen H. Computational Simulation, Multi-Spectroscopic and Degradation Analysis of the Interaction between Trametes versicolor Laccase and Bisphenol E. Zhurnal Prikladnoii Spektroskopii. 2023;90(2):352-1 - 352-10.
Views: 107
Email this article 