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Structural and Functional Assessment of the Human Skin Fibroblasts in a Free State and as Part of a Biomedical Cell Product Using Probe Fluorescence
Abstract
The key biophysical characteristics of human skin fibroblasts in a free state and as a part of a biomedical cell product (cells with a carrier “Collost” gel) were studied using flow cytometry and fluorescent probes. Data were obtained on the preservation of the intact state of fibroblasts in combination with the carrier, namely, the content of ionized calcium, reactive oxygen species in the cytoplasm of cells and the level of microviscosity of the lipid bilayer of cell membranes. It is shown that the “Collost” gel does not induce oxidative stress in fibroblasts, cell membranes are not damaged and there is no increase in the content of calcium ions in the cytoplasm of fibroblasts, the microviscosity of the lipid bilayer of membranes does not change, which is important for the use of the “Collost” gel carrier for the formation of a biomedical cell product and its subsequent use in cell therapy.
About the Authors
A. V. Butenka
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Minsk
S. V. Pinchuk
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Minsk
I. D. Volotovski
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Minsk
References
1. S. Ahn, H. Yoon, G. Kim, Y. Kim, S. Lee, W. Chun. Tissue Eng. C, Methods, 16, N 5 (2010) 813—820, https://doi.org/10.1089/ten.tec.2009.0511
2. A. Rahmanian-Schwarz, M. Held, T. Knoeller, S. Stachon, T. Schmidt, H. E. Schaller, L. Just. J. Biomed. Mater. Res., 102 (2014) 1173—1179, https://doi.org/10.1002/jbm.a.34793
3. K. Gelse, E. Poschl, T. Aigner. Adv. Drug Del. Rev., 55 (2003) 1531—1546, https://doi.org/10.1016/j.addr.2003.08.002
4. J. Glowacki, S. Mizuno. Biopolym. Orig. Res. Biomol. (2008) 338—344, https://doi.org/10.1002/bip.20871
5. L. Cen, W. Liu, L. Cui, W. Zhang, Y. Cao. Pediatr. Res., 63 (2008) 492—496, https://doi.org/10.1203/PDR.0b013e31816c5bc3
6. A. Ch. Chasnoit, I. B. Vasilevich, Z. B. Kvacheva, A. V. Butenka, K. A. Matievsky, V. M. Basalai, T. E. Vladimirskaya, V. N. Podgaisky, I. D. Volotovsky. J. Medicine, 3 (2022) 24—29, https://doi.org/10.51922/1818-426X.2022.3.24
7. E. Okazaki, L. Chikahisa, K. Kanemarul, Y. Oyama. Jpn. J. Pharmacol., 71 (1996) 273—280, https://doi.org/10.1254/jjp.71.273
8. C. Cerella, C. Mearelli, S. Coppola, M. D'Alessio, M. De Nicola, M. Diederich, L. Ghibelli. Apoptosis, 12 (2007) 2207—2219, https://doi.org/10.1007/s10495-007-0134-0
9. З. Б. Квачева, А. В. Бутенко, И. Б. Василевич, И. Д. Волотовский. Способ получения фибробластов кожи человека для трансплантации в условиях культуры, патент РБ № 18841 (2015)
10. A. Minta, J. P. Y. Kao, R. Y. J. Tsien. Biol. Chem., 264 (1989) 8171—8178
11. J. P. Y. Kao, A. T. Harootunian, R. Y. Tsien. J. Biol. Chem., 264 (1989) 8179—8184
12. O. Thastrup, P. J. Cullen, B. K. Drohak, M. R. Hanley, A. P. Dawson. Proc. Natl. Acad. Sci., 87 (1990) 2466—2470
13. N. V. Kalmykova, A. A. Andreev-Andrievsky, I. A. Demyanenko, V. I. Manskikh, E. A. Lagereva, A. S. Popova, Yu. S. Hats, A. P. Suslov. Biomedicine, 4 (2017) 85—96
14. M. M. Karasev, M. A. Redina, O. V. Belousova. Pharmacy and Pharmacology, 5, N 12 (2015) 12—17, https://doi.org/10.19163/2307-9266-2015-3-5(12)-12-17
15. L. I. Budkevich, V. I. Kovalchuk, A. V. Glutkin, M. A. Brazol, G. V. Mirzoyan, A. A. Gnipov, P. V. Salisty, Yu. V. Chekishev, A. A. Shmyrin, R. B. Gabitov. Russ. Bull. Pediatric Surgery, Anesthesiology and Resuscitation, 8, N 3 (2018) 34—44, https://doi.org/10.30946/2219-4061-2018-8-3-34-44
16. G. V. Lutsenko, M. V. Grechikhina, L. G. Dyachkova, N. I. Lutsan. Cytology, 49 (2007) 284—291
17. P. G. Winyard, C. J. Moody, C. Jacob. Trends Biochem. Sci., 30 (2005) 453—461, https://doi.Org/10.1016/j.tibs.2005.06.001
18. S. Sharma, O. Dewald, J. Adroque, R. L. Salazar. Free Rad. Biol. Med., 40 (2006) 2223—2231, https://doi.org/10.1016/j.freeradbiomed.2006.02.019
19. K. Luczak, A. Balcerczyk, M. Soszynski, G. Bartosz. Cell Biol. Int., 28 (2004) 483—486, https://doi.org/10.1016/j.cellbi.2004.03.004
20. T. A. Kulagova, G. N. Semenkova, Z. B. Kvacheva, S. N. Cherenkevich. Cytology, 48 (2006) 900—905
21. T. P. A. Devasagayam, J. C. Tilak, T. K. Boloor. J. A. P. I., 52 (2004) 794—804
22. V. A. Kostyuk, A. I. Potapovich. Bioradicals and Bioantioxidants, Minsk, BSU (2004)
23. E. A. Chernitsky, A. V. Vorobei. Modern Success. Biol., 101 (1986) 100—114
24. P. V. Mokrushnikov. Proc. Novosibirsk State University of Architecture and Civil Engineering (Sibstrin), 21, N 1 (2018) 17—29
Review
For citations:
Butenka A.V., Pinchuk S.V., Volotovski I.D. Structural and Functional Assessment of the Human Skin Fibroblasts in a Free State and as Part of a Biomedical Cell Product Using Probe Fluorescence. Zhurnal Prikladnoii Spektroskopii. 2023;90(3):394-400. (In Russ.)
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