Structural and Functional Assessment of the Human Skin Fibroblasts in a Free State and as Part of a Biomedical Cell Product Using Probe Fluorescence

Abstract

The key biophysical characteristics of human skin fibroblasts in a free state and as a part of a biomedical cell product (cells with a carrier “Collost” gel) were studied using flow cytometry and fluorescent probes. Data were obtained on the preservation of the intact state of fibroblasts in combination with the carrier, namely, the content of ionized calcium, reactive oxygen species in the cytoplasm of cells and the level of microviscosity of the lipid bilayer of cell membranes. It is shown that the “Collost” gel does not induce oxidative stress in fibroblasts, cell membranes are not damaged and there is no increase in the content of calcium ions in the cytoplasm of fibroblasts, the microviscosity of the lipid bilayer of membranes does not change, which is important for the use of the “Collost” gel carrier for the formation of a biomedical cell product and its subsequent use in cell therapy.