SERS IMAGING OF NASOPHARYNGEAL CARCINOMA MARKERS USING AN ANTIBODY-CONJUGATED GOLD NANOPARTICLES PROBE
Abstract
Surface-enhanced Raman scattering (SERS) nanotags as an ultrasensitive nanoprobe is becoming popular for the detection of biomarkers. Herein, antibody-conjugated gold nanoparticles (AuNPs) were used to target LMP2A in an LMP2A-infected CNE2 cell line. SERS maps showed that the LMP2A was distributed around the cell, which was consistent with the results of immunofluorescence staining in the previous report. This location could be due to the specific binding of the bioconjugated nanotags to the receptors on the cell surface. However, the CNE2 cell line without LMP2A-infected showed no detectable signal at 1044 cm- 1 . The results demonstrated the potential feasibility of AuNPs nanotags as highly sensitive probes conjugated at the subcellular level for detection and localization of cancer markers in nasopharyngeal carcinoma (NPC).
About the Authors
J. -H. Li
School of Mechanical and Electrical Engineering, Shenzhen Institute of Information Technology
Russian Federation
Russian Federation
Y. . Du
Sun Yat-sen University Cancer Center
Russian Federation
Russian Federation
G. -K. Feng
Sun Yat-sen University Cancer Center
Russian Federation
Russian Federation
Y. -B. Du
School of Mechanical and Electrical Engineering, Shenzhen Institute of Information Technology
Russian Federation
Russian Federation
Y. -Q. Zhou
Sun Yat-sen University Cancer Center
Russian Federation
Russian Federation
M. -S. Zeng
School of Mechanical and Electrical Engineering, Shenzhen Institute of Information Technology
Russian Federation
Russian Federation
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Review
For citations:
Li J.-., Du Y., Feng G.-., Du Y.-., Zhou Y.-., Zeng M.-. SERS IMAGING OF NASOPHARYNGEAL CARCINOMA MARKERS USING AN ANTIBODY-CONJUGATED GOLD NANOPARTICLES PROBE. Zhurnal Prikladnoii Spektroskopii. 2017;84(5):731-736. (In Russ.)
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