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Application of Confocal Microscopy to Monitor the Structural Transformations in Nanopillars Based on DNA and CdSe/CdZnSe/ZnS Quantum Dots
Abstract
Prototypes of chip systems have been created in the form of nanopillars from DNA complexes with CdSe/CdZnSe/ZnS quantum dots immobilized on a plasmonic gold film using the technology of vacuum deposition and inorganic synthesis. The design of nanopillars and the presence of terminal DNA labeled with Cy3 cyanine dyes makes it possible to carry out the reaction of hybridization of this terminal strand with complementary DNA and to control this process by changing the surface-enhanced Raman scattering (SERS) signal and fluorescence. The effect of molecular recognition of complementary DNA is accompanied by a change in the SERS spectrum, an increase in the fluorescence intensity by a factor of 20, and a decrease in the fluorescence decay time.
About the Authors
I. G. Motevich
Yanka Kupala Grodno State University
Belarus
Belarus
Grodno
T. Erdem
Abdullah Gul University
Turkey
Turkey
Kokasinan, Kayseri
A. Akrema
Abdullah Gul University
Turkey
Turkey
Kokasinan, Kayseri
S. A. Maskevich
Belarusian State University, A.D. Sakharov International State Ecological Institute
Belarus
Belarus
Minsk
N. D. Strekal
Yanka Kupala Grodno State University
Belarus
Belarus
Grodno
References
1. В. Степуро, А. Суханова, В. Артемьев, Е. Устинович, Н. Стрекаль, С. Маскевич, О. Кулакович, К. Мочалов, И. Набиев, В. Олейников. Опт. и спектр., 100, № 6 (2006) 935—942
2. И. Г. Мотевич, Н. Д. Стрекаль, Я. В. Новицкий, С. А. Маскевич. Докл. НАН Беларуси, 52, № 1 (2008) 54—57
3. I. G. Motevich, N. D. Strekal, J. W. Nowicky, S. A. Maskevich. J. Appl. Spectr., 77 (2010) 394—399
4. B. A. Ranishenka, E. A. Ulashchik, A. S. Kruhlik, M. Yu. Tatulchenkov, A. V. Radchanka, V. V. Shmanai, M. V. Artemyev. J. Appl. Spectr., 86 (2019) 1064—1070
5. Н. Д. Стрекаль. Вестн. ГрГУ, 1, № 25 (2004) 62—66
6. G. Vasilyuk, S. Maskevich, I. Sveklo, G. Zanevsky, G. Gachko, N. Strekal. J. Mol. Struct., 410-411 (1997) 223—227
7. M. Adam, T. Erdem, G. M. Stachowski, Z. Soran-Erdem, J. F. L. Lox, C. Bauer, A. Eychmuller. ACS Appl. Mater. & Interfaces, 7, N 41 (2015) 23364—23371
8. T. Erdem, Z. Soran-Erdem, Y. Kelestemur, N. Gaponik, H. V. Demir. Opt. Express, 24, N 2 (2016) 74—84
9. A. Banerjee, Chl.Grazon, B. Nadal, T. Pons,Ya. Krishnan, B. Dubertret. Вioconjugate Chem., 26, N 8 (2015) 1582—1589
10. A. Pal, Ya. Levy. PLoS Comput. Biol., 1 (2019) 15(1—4)
11. G. A. Gachko, V. K. Zybel't, L. N. Kivach, S. A. Maskevich, A. A. Mas- kevich. J. Appl. Spectr., 47 (1987) 849—852
12. A. Barhoumi, D. Zhang, F. Tam, N. J. Halas. J. Am. Chem. Soc., 130, N 16 (2008) 5523—5529
13. В. В. Железняков. УФН, 159, № 2 (1989) 193—260
Review
For citations:
Motevich I.G., Erdem T., Akrema A., Maskevich S.A., Strekal N.D. Application of Confocal Microscopy to Monitor the Structural Transformations in Nanopillars Based on DNA and CdSe/CdZnSe/ZnS Quantum Dots. Zhurnal Prikladnoii Spektroskopii. 2023;90(3):456-461. (In Russ.)
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