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Mechanism of Binding of Methylene Blue with Single-Stranded Synthetic Polynucleotides Poly(rA) and Poly(rU)
Abstract
The study of the binding of thiazine dye methylene blue (MB) with single-stranded (ss-) synthetic polyribonucleotides poly(rA) and poly(rU) has been carried out with the methods of absorption spectroscopy and UV-melting. It has been revealed that the binding mechanisms of MB to poly(rA) significantly differs from that one of MB binding to poly(rU). The obtained data indicate that the main binding mode of MB to ss-poly(rA) corresponds to semi-intercalation, while at the binding to poly(rU) — electrostatic binding. MB has been also found out to contribute to the formation of hybrid duplex poly(rA)-poly(rU).
About the Authors
Z. O. Movsesyan
Yerevan State University
Armenia
Armenia
Yerevan
A. P. Antonyan
Yerevan State University
Armenia
Armenia
Yerevan
P. O. Vardevanyan
Yerevan State University
Armenia
Armenia
Yerevan
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Review
For citations:
Movsesyan Z.O., Antonyan A.P., Vardevanyan P.O. Mechanism of Binding of Methylene Blue with Single-Stranded Synthetic Polynucleotides Poly(rA) and Poly(rU). Zhurnal Prikladnoii Spektroskopii. 2023;90(4):547-552. (In Russ.)
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